Characterization of messenger RNA expression of estrogen receptor-α and -β in patients with ovarian endometriosis Sachiko Matsuzaki, M.D., Takao Fukaya,

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Characterization of messenger RNA expression of estrogen receptor-α and -β in patients with ovarian endometriosis Sachiko Matsuzaki, M.D., Takao Fukaya, M.D., Shigeki Uehara, M.D., Takashi Murakami, M.D., Hironobu Sasano, M.D., Akira Yajima Fertility and Sterility Volume 73, Issue 6, Pages 1219-1225 (June 2000) DOI: 10.1016/S0015-0282(00)00527-6

Figure 1 Schematic representation of the structures of the ER-α– and ER-β–coding regions (complementary DNA) and the locations of the primers used for RT-PCR analysis. The positions of the translation initiation (ATG) and termination (TGA) codons are indicated. Black rectangles indicate the primer locations. Exon boundaries are shown as vertical bars. F = forward primer; R = reverse primer. Matsuzaki. ER-α and -β in endometriosis. Fertil Steril 2000. Fertility and Sterility 2000 73, 1219-1225DOI: (10.1016/S0015-0282(00)00527-6)

Figure 2 The RT-PCR detection of ER-α and ER-β mRNA in two samples of ovarian endometriotic tissues. The molecular sizes of the specific bands are indicated. Bands, of which molecular sizes were <100 bp, were derived probably from primer dimers. (A), from case no. 6 and (B) from case no. 19 in Table 3. M = molecular size marker (100-bp ladder); lane 1, RT-PCR for ER-α mRNA; lane 2, for ER-β mRNA; lane 3, for β-actin mRNA; and lane 4, negative control without RT. Matsuzaki. ER-α and -β in endometriosis. Fertil Steril 2000. Fertility and Sterility 2000 73, 1219-1225DOI: (10.1016/S0015-0282(00)00527-6)

Figure 3 In situ localization of ER-α and ER-β mRNA in an ovarian endometriotic cyst obtained from a patient (case no. 6 in Table 3). The ISH was performed on adjacent sections of an ovarian endometriotic cyst with an antisense probe to ER-α (a) and an antisense probe to ER-β (b). Both ER-α and ER-β mRNA hybridization signals, appearing red as a result of fast red, were detected in glandular epithelial and stromal cells. Negative controls with a sense probe to ER-α (c) or a sense probe to ER-β (d) showed no detectable specific mRNA hybridization signals. Bar = 50 μm. Matsuzaki. ER-α and -β in endometriosis. Fertil Steril 2000. Fertility and Sterility 2000 73, 1219-1225DOI: (10.1016/S0015-0282(00)00527-6)

Figure 4 In situ localization of ER-α and ER-β mRNA in an ovarian endometriotic cyst obtained from a patient (case no. 19 in Table 3). The ISH was performed on adjacent sections of an ovarian endometriotic cyst with an antisense probe to ER-α (a) and an antisense probe to ER-β (b). The ER-α hybridization signals, appearing red as a result of fast red, were detected in glandular epithelial and stromal cells. No ER-β hybridization signals were detected. Bar = 50 μm. Matsuzaki. ER-α and -β in endometriosis. Fertil Steril 2000. Fertility and Sterility 2000 73, 1219-1225DOI: (10.1016/S0015-0282(00)00527-6)