Erica E. Marsh, M. D. , Zhihong Lin, Ph. D. , Ping Yin, Ph. D

Slides:



Advertisements
Similar presentations
Control of Gene Expression
Advertisements

Date of download: 7/10/2016 Copyright © 2016 McGraw-Hill Education. All rights reserved. Basic steps of gene expression—transcription factors regulate.
Investigation of gene expression profiles before and after embryonic genome activation and assessment of functional pathways at the human metaphase II.
From: Emerging Roles for MicroRNAs in Perioperative Medicine
Schematic representation of the microRNA biogenesis machinery.
Biogenesis of micro (mi) and silencing (si)RNAs
Figure Legend: From: Noncoding RNAs:New Players in Chronic Pain
M. Louise Hull, Victoria Nisenblat  Reproductive BioMedicine Online 
miRNA genomic organization, biogenesis and function
Sandrine Giscard d’Estaing, Ph. D. , Delphine Perrin, M. Sc
MicroRNA-mediated DNA methylation in plants
Sphingosine pathway deregulation in endometriotic tissues
M. Louise Hull, Victoria Nisenblat  Reproductive BioMedicine Online 
Sandrine Giscard d’Estaing, Ph. D. , Delphine Perrin, M. Sc
Differential expression of G-protein-coupled estrogen receptor-30 in human myometrial and uterine leiomyoma smooth muscle  Ruijuan Tian, M.Sc., Zengyong.
Whole genome deoxyribonucleic acid microarray analysis of gene expression in ectopic versus eutopic endometrium  Kathleen M. Eyster, Ph.D., Olga Klinkova,
Testicular expression of synaptonemal complex protein 3 (SYCP3) messenger ribonucleic acid in 110 patients with nonobstructive azoospermia  Mahmoud Aarabi,
Fertility and Sterility
MicroRNA expression in the human blastocyst
How MicroRNAs Modify Protein Production
Potential role of circulating microRNAs as a biomarker for unexplained recurrent spontaneous abortion  Weibing Qin, M.D., Ph.D., Yunge Tang, M.D., Ning.
Increased expression of platelet-derived growth factor C messenger ribonucleic acid in uterine leiomyomata  Yuh-Ming Hwu, M.D., Sheng-Hsiang Li, Ph.D.,
New Hope for a MicroRNA Therapy for Liver Cancer
MicroRNAs: regulators of gene expression and cell differentiation
C. Belair, F. Darfeuille, C. Staedel 
The selective progesterone receptor modulator CDB4124 inhibits proliferation and induces apoptosis in uterine leiomyoma cells  Xia Luo, Ph.D., Ping Yin,
Rafael Kramann, Marcus J. Moeller  Kidney International 
Expression of insulin-like growth factors (IGFs) and IGF signaling: molecular complexity in uterine leiomyomas  Lan Peng, M.D., Yong Wen, M.D., Yulong.
Expression of forkhead transcription factors in human granulosa cells
Aromatase, microRNA, and inflammation: a complex relationship
Follicular fluid and mural granulosa cells microRNA profiles vary in in vitro fertilization patients depending on their age and oocyte maturation stage 
MicroRNAs in the Pathogenesis of Lung Cancer
Estrogen receptor β regulates endometriotic cell survival through serum and glucocorticoid–regulated kinase activation  Diana Monsivais, Ph.D., Matthew.
Volume 84, Issue 6, Pages (December 2013)
Prospective assessment of midsecretory endometrial leukemia inhibitor factor expression versus ανβ3 testing in women with unexplained infertility  Jason.
HMGA2, MicroRNAs, and Stem Cell Aging
MicroRNAs: Essential players in the regulation of inflammation
J. Browning Fitzgerald, B. S. , Vargheese Chennathukuzhi, Ph. D
Studies of gene expression in human cumulus cells indicate pentraxin 3 as a possible marker for oocyte quality  Xingqi Zhang, Ph.D., Nadereh Jafari, Ph.D.,
Submucosal uterine leiomyomas have a global effect on molecular determinants of endometrial receptivity  Beth W. Rackow, M.D., Hugh S. Taylor, M.D.  Fertility.
Reply of the Authors Fertility and Sterility
Endometrial LGR7 expression during menstrual cycle
MicroRNAs in Liver Disease
Adenovirus-mediated expression of cyclooxygenase-2 antisense reverse abnormal genetic profile of human adhesion fibroblasts  Ghassan M. Saed, Ph.D., Ayman.
Heparin inhibits interferon-γ signaling in human endometrial stromal cells by interference with the cellular binding of interferon-γ  Herbert Fluhr, M.D.,
Rahi Victory, M. D. , F. R. C. S. C. , Ghassan M. Saed, Ph. D
Gyongyi Szabo, Peter Sarnow, Shashi Bala  Journal of Hepatology 
siRNA Versus miRNA as Therapeutics for Gene Silencing
MicroRNA Functions in Stress Responses
MicroRNAs in cancer: biomarkers, functions and therapy
Molecular Therapy - Nucleic Acids
Jieqiang Lv, M. D. , Xueqiong Zhu, M. D. , Ph. D
A) Primary microRNA (pri-microRNA) is processed by Drosha in the nucleus to form pre-microRNA. a) Primary microRNA (pri-microRNA) is processed by Drosha.
Development and validation of a three-dimensional in vitro model for uterine leiomyoma and patient-matched myometrium  Minnie Malik, Ph.D., William H.
Markus Bitzer, Iddo Z. Ben-Dov, Thomas Thum  Kidney International 
Engineering parathyroid cells to treat secondary hyperparathyroidism
MicroRNA as Therapeutic Targets for Chronic Wound Healing
Microarray analysis in sperm from fertile and infertile men without basic sperm analysis abnormalities reveals a significantly different transcriptome 
Epidermal growth factor–containing fibulin-like extracellular matrix protein 1 expression and regulation in uterine leiomyoma  Erica E. Marsh, M.D., M.S.C.I.,
Human myometrium and leiomyomas express gonadotropin-releasing hormone 2 and gonadotropin-releasing hormone 2 receptor  Jason D. Parker, D.O., Minnie.
Volume 139, Issue 1, Pages (October 2009)
Molecular profiling of experimental endometriosis identified gene expression patterns in common with human disease  Idhaliz Flores, Ph.D., Elizabeth Rivera,
Identification of differential gene expression in germinal vesicle vs
The selective progesterone receptor modulator CDB4124 inhibits proliferation and induces apoptosis in uterine leiomyoma cells  Xia Luo, Ph.D., Ping Yin,
MicroRNA: key gene expression regulators
MicroRNAs in cancer: biomarkers, functions and therapy
Cellular biogenesis of microRNAs.
Jason Yen-Ping Ho, M. D. , Weng Chi Man, Ph. D. , Yan Wen, M. D
MicroRNA-22-3p is down-regulated in the plasma of Han Chinese patients with premature ovarian failure  Yujie Dang, M.D., Ph.D., Shidou Zhao, Ph.D., Yingying.
Endometrial polyps affect uterine receptivity
Presentation transcript:

Differential expression of microRNA species in human uterine leiomyoma versus normal myometrium  Erica E. Marsh, M.D., Zhihong Lin, Ph.D., Ping Yin, Ph.D., Magdy Milad, M.D., Debabrata Chakravarti, Ph.D., Serdar E. Bulun, M.D.  Fertility and Sterility  Volume 89, Issue 6, Pages 1771-1776 (June 2008) DOI: 10.1016/j.fertnstert.2007.05.074 Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 1 MicroRNA cellular pathway. The gene for the miRNA is transcribed by RNA polymerase II into a single strand of RNA. This single strand folds into a hairpin-like structure and becomes the double-stranded primary miRNA (pri-miRNA). The hairpin-like structure is subsequently cleaved in the nucleus by Drosha, forming the pre-miRNA. The pre-miRNA then enters the cytoplasm via the protein channel Exportin, where it is cleaved into a small piece of double-stranded RNA by the enzyme Dicer. The double-stranded small RNA then separates into single strands, with one strand being degraded and the other strand forming a complex with Argonaute proteins. This newly formed protein/nucleic acid complex is called an RNA-induced silencing complex (RISC). These complexes then either bind messenger RNA (mRNA) with exact complementarity, leading to cleavage of the mRNA by the RISC, or bind with partial complementarity, leading to repression of translation. Fertility and Sterility 2008 89, 1771-1776DOI: (10.1016/j.fertnstert.2007.05.074) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Real-time reverse transcriptase polymerase chain reaction (RT-PCR) validation of microarray. Real-time semiquantitative RT-PCR of five different microRNA species (miR-21, miR-34a, miR-125b, miR-139, and miR-323) across ten subjects. miR-21, miR-34a, miR-125b, and miR-323 were overexpressed in leiomyoma in the ten pairs tested and miR-139 was underexpressed, consistent with the microarray results. Fertility and Sterility 2008 89, 1771-1776DOI: (10.1016/j.fertnstert.2007.05.074) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions