Temporal genomics of vein bypass grafting through oligonucleotide microarray analysis Jeffrey A Kalish, MD, David J Willis, MD, Cheng Li, PhD, Jeffrey.

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Volume 141, Issue 3, Pages (March 2012)

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Presentation transcript:

Temporal genomics of vein bypass grafting through oligonucleotide microarray analysis Jeffrey A Kalish, MD, David J Willis, MD, Cheng Li, PhD, Jeffrey J Link, BA, Evan R Deutsch, MD, Mauricio A Contreras, MD, William C Quist, MD, PhD, Frank W Logerfo, MD Journal of Vascular Surgery Volume 39, Issue 3, Pages 645-654 (March 2004) DOI: 10.1016/j.jvs.2003.10.049

Fig 1 dChip hierarchical clustering diagram shows sample clustering. Each row corresponds to a single gene; each column corresponds to a single sample. Columns to the left of midline are one batch of arrays (days 1 and 7); columns to the right are the other batch (days 14 and 30). Blue, Degrees of downregulation; red, degrees of upregulation; H, hyperplasia samples (vein grafts); C, control samples (control veins). Journal of Vascular Surgery 2004 39, 645-654DOI: (10.1016/j.jvs.2003.10.049)

Fig 2 Graphs show quantitative real-time reverse transcriptase polymerase chain reaction. A, One-day vein graft (blue) shows 0.75 times the expression of HIF-1α as control vein (red). B, One-day vein graft (blue) shows 0.30 times the expression of 18S as control vein (red). Normalizing HIF-1α results by 18S reveal 2.5-fold upregulation of HIF-1α in vein graft vs control vein, confirming the microarray data. C, Seven-day vein graft (blue) shows 0.22 times the expression of HIF-1α as control vein (red). D, Seven-day vein graft (blue) shows 0.25 times the expression of 18S as control vein (red). Normalization reveals return of HIF-1α to baseline levels in vein graft vs control vein. Journal of Vascular Surgery 2004 39, 645-654DOI: (10.1016/j.jvs.2003.10.049)

Fig 3 Hematoxylin-eosin staining. A, Control vein (×400). B, At 7 days vein graft shows intimal hyperplasia (×100). C, At 14 days vein graft shows medial hypertrophy and intimal hyperplasia (×100). D, At 30 days vein graft shows medial hypertrophy and extracellular matrix deposition (×100). L, Lumen. Journal of Vascular Surgery 2004 39, 645-654DOI: (10.1016/j.jvs.2003.10.049)

Fig 4 Immunohistochemical staining to qualitatively localize protein expression of VEGF (A-C), Ki-67 (D, E), and actin (F-H) at 1, 7, and 30 days (X 100). L, = Lumen. Journal of Vascular Surgery 2004 39, 645-654DOI: (10.1016/j.jvs.2003.10.049)