Recovery of Circulating T-Follicular Helper Cells after Hematopoietic Stem Cell Transplantation  Edouard Forcade, MD, Steven Paula, Kristen Cowens, Marta Leal Bento, Sarah Nikiforow, MD PhD, John Koreth, MBBS, DPhil, Corey S. Cutler, MD, Vincent T. Ho, MD, Philippe Armand, MD, PhD, Edwin P. Alyea, MD, Robert J. Soiffer, MD, Joseph H. Antin, MD, Bruce R. Blazar, MD, Jerome Ritz, MD  Biology of Blood and Marrow Transplantation  Volume 21, Issue 2, Pages S164-S165 (February 2015) DOI: 10.1016/j.bbmt.2014.11.240 Copyright © 2015 Terms and Conditions

Figure 1 (Left) Recovery of tTFH and CD4+ T-cells/μL) after HSCT. (Right) Frequencies of cTFH in the CD4+ T-cell gate beginning 2 months after HSCT. Number of patients analyzed for each time point is indicated below the graph. Dashed lines represent the median values from 11 Healthy Donors (90% CI plotted in light red and light grey for the respective population). Biology of Blood and Marrow Transplantation 2015 21, S164-S165DOI: (10.1016/j.bbmt.2014.11.240) Copyright © 2015 Terms and Conditions

Figure 2 Assessment of B-tell helper function in cTFH subsets. cTFH (CD4+CD45RA-CXCR5+) and non-cTFH (CD4+CD45RA-CXCR5-) T cell subsets were purified by flow cytometric cell sorting. IgG (left) and IgM (Right) production was measured in supernatants? after 12-day co-culture of purified T-cell subsets with naive B-cells stimulated with Staphylococcal Enterotoxin B. Biology of Blood and Marrow Transplantation 2015 21, S164-S165DOI: (10.1016/j.bbmt.2014.11.240) Copyright © 2015 Terms and Conditions

Figure 3: Comparison of Th2+Th17 cTFH (Left) and (Th2+Th17)/Th1 cTFH ratio (Right) in patients without cGVHD (N=38) and with cGVHD (N=33). Biology of Blood and Marrow Transplantation 2015 21, S164-S165DOI: (10.1016/j.bbmt.2014.11.240) Copyright © 2015 Terms and Conditions