Mitochondrial occupancy decreases presynaptic Ca2+ signals

Slides:

Advertisements

Similar presentations

Volume 70, Issue 4, Pages (May 2011)

Advertisements

Christian Rosenmund, Charles F Stevens Neuron

JNK is activated by Wnt5a.

GABAA Receptors at Hippocampal Mossy Fibers

Loss of OCTR‐1 signalling enhances the activation of XBP‐1‐mediated UPR pathway. Loss of OCTR‐1 signalling enhances the activation of XBP‐1‐mediated UPR.

The activation of caspase‐3 and depolarization of the mitochondrial inner membrane are very rapid and occur in parallel. The activation of caspase‐3 and.

Subcellular localization of full‐length and cleaved PINK1 PINK1 immunoblot of cytoplasmic and mitochondrial fractions from HeLa cells transfected with.

Overexpression of RNase H1 decreases the induction of phosphorylated H2AX in post‐mitotic cells in response to topoisomerase 1 cleavage complexes. Overexpression.

Alla Y. Karpova, Dougal G.R. Tervo, Noah W. Gray, Karel Svoboda Neuron

TUG‐891 increases oxygen consumption by brown adipocytes, mediated by direct UCP1 activation and mitochondrial fragmentation TUG‐891 increases oxygen consumption.

Functional Convergence at the Retinogeniculate Synapse

Burst-Timing-Dependent Plasticity of NMDA Receptor-Mediated Transmission in Midbrain Dopamine Neurons Mark T. Harnett, Brian E. Bernier, Kee-Chan Ahn,

Control of Inhibitory Synaptic Outputs by Low Excitability of Axon Terminals Revealed by Direct Recording Shin-ya Kawaguchi, Takeshi Sakaba Neuron

IRF4 is required for anabolic induction of CD4+ T cells.

Volume 100, Issue 3, Pages (February 2011)

Daniel Meyer, Tobias Bonhoeffer, Volker Scheuss Neuron

Volume 99, Issue 9, Pages (November 2010)

The STM1787 promoter in Salmonella is rapidly activated in vivo by the tumor microenvironment. The STM1787 promoter in Salmonella is rapidly activated.

Michael B. Hoppa, Geraldine Gouzer, Moritz Armbruster, Timothy A. Ryan

Bidirectional Modification of Presynaptic Neuronal Excitability Accompanying Spike Timing-Dependent Synaptic Plasticity Cheng-yu Li, Jiang-teng Lu, Chien-ping.

Stanislav S Zakharenko, Leonard Zablow, Steven A Siegelbaum Neuron

Rab22A localizes to the E/SE and REs and regulates RE dynamics

Volume 11, Issue 12, Pages (June 2015)

Recruitment of N-Type Ca2+ Channels during LTP Enhances Low Release Efficacy of Hippocampal CA1 Perforant Path Synapses Mohsin S. Ahmed, Steven A. Siegelbaum

Volume 87, Issue 6, Pages (December 1996)

Arc expression increases H4K12Ac levels.

Subcellular Dynamics of Type II PKA in Neurons

Volume 21, Issue 2, Pages (October 2017)

Katherine M. Armstrong, Jamie K. Fitzgerald, Tirin Moore Neuron

Visualization of Subunit-Specific Delivery of Glutamate Receptors to Postsynaptic Membrane during Hippocampal Long-Term Potentiation Hiromitsu Tanaka,

Volume 60, Issue 4, Pages (November 2008)

Feng Han, Natalia Caporale, Yang Dan Neuron

Tao Sun, Haifa Qiao, Ping-Yue Pan, Yanmin Chen, Zu-Hang Sheng

Sung E. Kwon, Edwin R. Chapman Neuron

Endocannabinoids Mediate Neuron-Astrocyte Communication

Volume 50, Issue 3, Pages (May 2006)

Stephan D. Brenowitz, Wade G. Regehr Neuron

Bo Li, Ran-Sook Woo, Lin Mei, Roberto Malinow Neuron

Tiago Branco, Kevin Staras, Kevin J. Darcy, Yukiko Goda Neuron

Matthew S. Kayser, Mark J. Nolt, Matthew B. Dalva Neuron

Marta Navarrete, Alfonso Araque Neuron

Dario Maschi, Vitaly A. Klyachko Neuron

Differentiating Cerebellar Impact on Thalamic Nuclei

TUG‐891 increases oxygen consumption by brown adipocytes, mediated by direct UCP1 activation and mitochondrial fragmentation TUG‐891 increases oxygen consumption.

ZBTB48 is required for MTFP1 expression

Rescue of motor neurons from death The ventral root of the fifth lumbar segment (L5). Rescue of motor neurons from death The ventral root of the fifth.

Volume 57, Issue 3, Pages (February 2008)

Inhibition of endogenous Shh pathway reduces epileptiform activities without affecting physiological synaptic transmission Inhibition of endogenous Shh.

Volume 44, Issue 5, Pages (December 2004)

PKAN human neurons show altered oxidative status

Tangle formation and downregulation of miR‐132‐3p in LOAD A‐G

Volume 17, Issue 2, Pages (October 2016)

Volume 132, Issue 1, Pages (January 2008)

Christian Rosenmund, Charles F Stevens Neuron

Burst-Timing-Dependent Plasticity of NMDA Receptor-Mediated Transmission in Midbrain Dopamine Neurons Mark T. Harnett, Brian E. Bernier, Kee-Chan Ahn,

Sydney Cash, Yang Dan, Mu-ming Poo, Robert Zucker Neuron

Detection and classification of dendritic spines in transfected hippocampal neurons. Detection and classification of dendritic spines in transfected hippocampal.

Knockdown of ZBP1 in hippocampal neurons decreases dendritic branching

Won Chan Oh, Laxmi Kumar Parajuli, Karen Zito Cell Reports

Ribosomal RNA and β-catenin mRNA localize to newly formed presynaptic terminals. Ribosomal RNA and β-catenin mRNA localize to newly formed presynaptic.

Volume 57, Issue 3, Pages (February 2008)

PDL-coated beads induce the formation of functional presynaptic terminals within 24 h. PDL-coated beads induce the formation of functional presynaptic.

Ziv Gil, Barry W Connors, Yael Amitai Neuron

Knock-out of NSG2 decreases mEPSC frequency.

SSC LTP in narrow-field vertical neurons is induced by SO stimulation at 20 Hz for 20 s. sSC LTP in narrow-field vertical neurons is induced by SO stimulation.

Venkatesh N Murthy, Thomas Schikorski, Charles F Stevens, Yongling Zhu

Cotransport of GRAB with ca Rab11A in an axon.

Juliana M. Rosa, Sabine Ruehle, Huayu Ding, Leon Lagnado Neuron

One-photon characterization of RVF5 in neurons.

Gwendolyn G. Calhoon, Patricio O’Donnell Neuron

Presentation transcript:

Mitochondrial occupancy decreases presynaptic Ca2+ signals Mitochondrial occupancy decreases presynaptic Ca2+ signals ALive images of neurons co‐transfected with SyGCaMP5 and MtDsRed before and during 10 Hz field stimulation. The full arrow indicates a mitochondrially occupied terminal and the empty arrow a terminal without a mitochondrion. Scale bar, 10 μm.BAverage ΔF/F0 SyGCaMP5 traces from n = 11 neurons (98 terminals) plotting the average of terminals without a mitochondrion (black trace) and with a mitochondrion (red trace). Stimulation occurred for 10 s (t = 20–30) at 10 Hz.CAverage Ca2+ response following stimulation in terminals with or without a mitochondrion (average of ΔF measurement taken for a Δt = 20–30 s of B), ΔF/F0 = 1.9 ± 0.3 with and 3.5 ± 0.4 without mitochondria, paired t‐test, ***P < 0.0001.D, EThere is no significant difference in the presynaptic Ca2+ response in terminals with and without a mitochondrion in response to single action potentials. (D) Example trace of hippocampal neurons transfected with MtDsRed and SyGCaMP5. 1 ms stimulation pulses were applied every 5 s. The red trace represents an average of the terminals occupied with a mitochondrion in this neuron, whereas the black trace represents the averaged response of terminals without a mitochondrion. (E) Maximal fluorescence intensity in terminals without a mitochondrion (left) or with a mitochondrion (right) within the same axon (dots connected by a line) (n = 8 neurons, 44 terminals, paired t‐test, P = 0.55).FAverage ΔF/F0 SyGCaMP5 traces for 80 stimuli (n = 7 neurons, 21 terminals, Δt = 20–28 s, ΔF/F0 = 1.3 ± 0.2 with and 2.2 ± 0.5 without mitochondria, paired t‐test, *P < 0.05), 40 stimuli (n = 5 neurons, 25 terminals, Δt = 20–24 s, ΔF/F0 = 0.9 ± 0.3 with and 2.0 ± 0.4 without mitochondria, paired t‐test, *P < 0.05), 20 stimuli (n = 10 neurons, 55 terminals, Δt = 20–22 s, ΔF/F0 = 0.8 ± 0.4 with and 1.8 ± 0.4 without mitochondria, paired t‐test, *P < 0.05) and 10 stimuli (n = 4 neurons, 21 terminals, Δt = 20–21 s, ΔF/F0 = 0.4 ± 0.3 with and 0.4 ± 0.1 without mitochondria, paired t‐test, P = 0.90), all delivered at 10 Hz.Data information: Experiments were performed in E18 or P0 rat hippocampal neuronal cultures at DIV 10–12. Error bars represent SEM. Victoria Vaccaro et al. EMBO Rep. 2017;18:231-240 © as stated in the article, figure or figure legend