Strategies for the detection of Salmonella and Shigella on routine stool cultures. Strategies for the detection of Salmonella and Shigella on routine stool.

Slides:

Advertisements

Similar presentations

Enter question text... 1.Enter answer text.... Enter question text... 1.Enter answer text...

Advertisements

Enter question text... 1.Enter answer text.... Enter question text... 1.Enter answer text...

BRITISH SOCIETY FOR MICROBIAL TECHNOLOGY SCIENTIFIC WORKSHOP Enterics – a bog standard approach? J.D. Perry Freeman Hospital Newcastle upon Tyne An Appraisal.

Microbiology Lab # 11 Medgar Evers College Dr. Santos

Current position of MALDI-TOF MS in the workflow of the clinical microbiology laboratory, including the current options for analysis of bacteria directly.

Patrick R. Murray The Journal of Molecular Diagnostics

Sections of Columbia blood agar plates showing grayish, hemolytic colonies of S. haemolyticus (the color of this photograph was modified to enhance visibility.

Itraconazole tissue and fluid concentrations in humans as multiples of the maximal or simultaneously measured concentration in plasma (μg/ml) after systemic.

(A) Fractional probability of target attainment (expressed as a percentage) of intravenous levofloxacin at 500 mg daily, based on PK in healthy volunteers.

Novel, Improved Sample Preparation for Rapid, Direct Identification from Positive Blood Cultures Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight.

Mechanisms by which microbial cells might develop resistance

Frequency of lesions detected using CNS imaging (computed tomography [CT] and magnetic resonance imaging [MRI]) on patients with baylisascariasis (see.

Pathogenic schemes of diarrheagenic E. coli.

Phylogenetic tree showing generic and species positions based on Bayesian analysis of the nuclear ribosomal DNA 28S region (1,200 bp) of Schistosomatidae.

Columbia blood agar plate showing an isogenic S

2-D gel images visualized by Coomassie Brilliant Blue staining representing total proteins extracted from HCT-8 under apoptotic conditions in 2 mm Gln.

Adenoviremia with multiple HAdV species.

Biphasic nature of leptospirosis and relevant investigations at different stages of disease. Biphasic nature of leptospirosis and relevant investigations.

S. aureus recovered from the airways of a cystic fibrosis patient.

Reports of natural Trypanosoma cruzi infection in U.S. mammals.

Temporal correlation between intestinal HAdV infection and viremia.

Comparative analysis of C. glabrata, C. albicans, and S

Glucose concentration-dependent increase of melanin synthesis.

Identification of the major molecular types within C. gattii.

Detection of Ebola virus infection in nonfatal versus fatal cases.

Proportions of patients with not serious, serious, or fatal disease with a diagnosis of P. falciparum or P. vivax infection and having parasitemias of.

Positivity rates and concordance of multiplex PCR and blood culture (BC) results from 27 published studies. Positivity rates and concordance of multiplex.

Fluconazole tissue and fluid concentrations in humans as multiples of the maximal or simultaneously measured concentration in plasma (μg/ml) after systemic.

Number of outbreaks associated with drinking water by water system type and year (n = 780), 1971 to “Other” includes outbreaks associated with bottled.

Percentages of outbreak deficiencies (n = 671) in public water systems (n = 656) by time period, 1971 to 2006, excluding outbreaks associated with Legionella.

Malaria mortality in the 20th century.

Representative results of nested PCR assays for direct detection of “T

Recommended scheme for determination of heteroresistance and interpretation criteria. Recommended scheme for determination of heteroresistance and interpretation.

(Top) Graph illustrating citations in books for Plasmodium falciparum and Plasmodium vivax from 1900 to 2008, generated by use of the tool at

Number of outbreaks associated with drinking water by system type and month (n = 762), 1971 to 2006, excluding outbreaks associated with commercially bottled.

The type strain of H. pittmaniae was cultured for 24 h on different agars in the presence of a NAD-containing disk and photographed by transillumination.

Fabrizio Dutly, and Martin Altwegg Clin. Microbiol. Rev. 2001; doi:10

Two different pharmacophoric domains attached covalently by a linker domain. Two different pharmacophoric domains attached covalently by a linker domain.

Amoeba culturing methods for recovering amoeba-resisting microorganisms (ARMs). Amoeba culturing methods for recovering amoeba-resisting microorganisms.

Diagnostic tools for intestinal pathogenic E. coli.

Hector H. García et al. Clin. Microbiol. Rev. 2002; doi: /CMR

General schematic for MS analysis of ionized microbiological isolates and clinical material. General schematic for MS analysis of ionized microbiological.

Publications on biosensors for the field in general compared with the specific detection of whole bacteria. Publications on biosensors for the field in.

Multiplex PCR assay to detect common resistance determinants in B

Malaria mortality in the 20th century.

Association of HIV-1 replication fitness, as measured in a whole-virus parallel infection assay, with ability to control viremia after treatment interruption.

Three different experimental setups for assessing malaria transmission and TRA. In direct skin feeding assays, reared (malaria-free) mosquitoes are allowed.

Immunohistochemical evidence for the expression of PERV in the white pulp of the spleen of a Yucatan miniature swine. Immunohistochemical evidence for.

Data from an active surveillance study performed in Ohio in 1991, showing age-specific rates of community-acquired pneumonia due to the major bacterial.

Roberta B. Carey et al. Clin. Microbiol. Rev. 2018; doi: /CMR

Western blot assay of an acute MIF-positive serum showing reactivity with the 135- and 115-kDa specific protein antigens of Rickettsia conorii and Rickettsia.

Identification algorithm combining the use of commercial DNA probes (rounded rectangles and squares) and genetic sequencing (ellipses). Identification.

General schematic for the identification of bacteria and yeast by MALDI-TOF MS using the intact-cell method. General schematic for the identification of.

The future TB clinic. The future TB clinic. Sputum, saliva, and fecal samples are collected from patients for genome sequencing, SNP typing, or metagenomic.

RNase protection assay illustrating multiple mRNAs elicited by microglia in response to Acanthamoeba. RNase protection assay illustrating multiple mRNAs.

Immunoblot assay (left panel) and whole-cell radioimmunoprecipitation (RIP) assay (right panel) with preexacerbation serum (lanes A) and postexacerbation.

The Scorpions primer is an extension of molecular beacons.

Heteroresistant versus homogeneous responses to antibiotics.

Specimen types, diagnostic tests, and anatomical locations for determining a potential current or recent Legionella infection. Specimen types, diagnostic.

Adherence patterns of enteropathogenic E

Gram-stained smear of S

Basic diagnostic algorithm to link the molecular line probe assay with solid culture- and liquid culture-based growth detection and susceptibility testing.

Significant differences in target copy numbers in multiplex PCRs may result in incorrect identification at high Cq values. Significant differences in target.

Phylogenetic tree of Shigella, EIEC, and nonpathogenic E

Distribution of the four major molecular types of C

Molecular surface models of the active-site cleft of OXA-66 (A) and OXA-40 (B), showing the absence of a hydrophobic bridge in OXA-66. Molecular surface.

Dendrogram clustering the MALDI-TOF MSP obtained from at least 20 mass spectra of strains belonging to the C. haemulonii complex species and related species.

Walk-away specimen processor (WASP).

Diagrammatic view of the locations of genes encoding classical HLA class I (HLA-A, -B, -C, -E, -F, and -G) and class II (DR, DQ, DP, and DM) molecules.

Genetic events contributing to the evolution of Shigella spp

Presentation transcript:

Strategies for the detection of Salmonella and Shigella on routine stool cultures. Strategies for the detection of Salmonella and Shigella on routine stool cultures. Where Hektoen enteric (HE) agar is specified, any agar listed in the text is appropriate. (A) Traditional approach to fecal stool culture workup for Salmonella and Shigella. See Table 3 for reactions of enteric pathogens. (B) Approach to identification of Salmonella and Shigella when using MALDI-TOF MS. See Table 4 for reactions of enteric pathogens. (C) Approach to identification of Salmonella and Shigella if chromogenic medium is used as part of the primary setup. (D) Approach to use of a multiplex molecular assay for the detection of Salmonella and Shigella. Romney M. Humphries, and Andrea J. Linscott Clin. Microbiol. Rev. 2015; doi:10.1128/CMR.00073-14