Monocyte-derived dendritic cell recruitment and allergic TH2 responses after exposure to diesel particles are CCR2 dependent Sharen Provoost, MSc, Tania.

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Monocyte-derived dendritic cell recruitment and allergic TH2 responses after exposure to diesel particles are CCR2 dependent Sharen Provoost, MSc, Tania Maes, PhD, Guy F. Joos, MD, PhD, Kurt G. Tournoy, MD, PhD Journal of Allergy and Clinical Immunology Volume 129, Issue 2, Pages 483-491 (February 2012) DOI: 10.1016/j.jaci.2011.07.051 Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 WT mice were exposed to saline (white bars) or 100 μg of DEPs (black bars). Monocytes in the lung interstitium (A) and the alveolar space (B) were determined by using flow cytometry. C-F, Lung CD11b− cDCs (Fig 1, C), CD11b+ cDCs (Fig 1, D), inflammatory DCs (Fig 1, E), and alveolar DCs (Fig 1, F) were also determined by using flow cytometry. Results are expressed as mean ± SEM. n = 8 mice per group. ∗∗∗P < .005. Journal of Allergy and Clinical Immunology 2012 129, 483-491DOI: (10.1016/j.jaci.2011.07.051) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 WT mice were exposed to saline (white bars) or 100 μg of DEPs (black bars). A-C, MCP-1 (Fig 2, A), RANTES (Fig 2, B), and MIP-3α (Fig 2, C) protein levels in the BALF were determined by using ELISA. D-F, CCR2 mRNA (Fig 2, D), CCR5 mRNA (Fig 2, E), and CCR6 mRNA (Fig 2, F) expression in the lung tissue was determined by using RT-PCR. Results are expressed as mean ± SEM. n = 8 mice per group. ∗∗∗P < .005. Journal of Allergy and Clinical Immunology 2012 129, 483-491DOI: (10.1016/j.jaci.2011.07.051) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 WT mice and CCR2 knockout mice were exposed to saline (white bars) or 100 μg of DEPs (black bars). Monocytes in the lung interstitium (A) and the alveolar space (B) were determined by using flow cytometry. C-F, Lung CD11b− cDCs (Fig 3, C), CD11b+ cDCs (Fig 3, D), inflammatory DCs (Fig 3, E), and alveolar DCs (Fig 3, F) were determined by using flow cytometry. Results are expressed as mean ± SEM. n = 7-8 mice per group. ∗∗∗P < .005. Journal of Allergy and Clinical Immunology 2012 129, 483-491DOI: (10.1016/j.jaci.2011.07.051) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 WT mice and CCR6 knockout mice were exposed to saline (white bars) or 100 μg of DEPs (black bars). Monocytes in the lung interstitium (A) and the alveolar space (B) were determined by using flow cytometry. C-F, Lung CD11b− cDCs (Fig 4, C), CD11b+ cDCs (Fig 4, D), inflammatory DCs (Fig 4, E), and alveolar DCs (Fig 4, F) were determined by using flow cytometry. Results are expressed as mean ± SEM. n = 7-8 mice per group. ∗P < .05, ∗∗P < .01, ∗∗∗P < .005. Journal of Allergy and Clinical Immunology 2012 129, 483-491DOI: (10.1016/j.jaci.2011.07.051) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 A, Representative dot plots of baseline circulating CD11b+Ly6C+ monocytes in WT, CCR2 knockout, and CCR6 knockout mice. B, Bone marrow cells from WT mice and CCR2 knockout mice were labeled with CFSE and intravenously injected into DEP-treated WT mice. Forty-eight hours later, CFSE+CD11b+ monocytes in the lung tissue were determined by using flow cytometry. C, Bone marrow cells from WT mice and CCR6 knockout mice were labeled with CFSE and intravenously injected into DEP-treated WT mice. Forty-eight hours later, CFSE+CD11b+ monocytes in the lung tissue were determined by using flow cytometry. Numbers on graphs represent mean percentage monocytes. Results are expressed as mean ± SEM (Fig 5, C). n = 7-8 mice per group. ∗P < .05, ∗∗∗P < .005. Journal of Allergy and Clinical Immunology 2012 129, 483-491DOI: (10.1016/j.jaci.2011.07.051) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 6 WT mice, CCR2 knockout mice, and CCR6 knockout mice were exposed to saline (white bars) or 100 μg of DEPs (black bars). On day 8, mice were intravenously injected with 10 × 106 OT-II cells and instilled with 350 μg OVA on day 9. Four days later, mediastinal LNs were digested and cultured in medium plus 100 μg/mL OVA. A-C, IL-4 (Fig 6, A), IL-10 (Fig 6, B), and IL-13 (Fig 6, C) protein levels in mediastinal LN supernatant were determined by using ELISA. Results are expressed as mean ± SEM. n = 7-8 mice per group; saline groups were pooled to make graphs more clear (there were no statistical differences between saline groups); ∗P < .05, ∗∗∗P < .005. Journal of Allergy and Clinical Immunology 2012 129, 483-491DOI: (10.1016/j.jaci.2011.07.051) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions