TR directly binds to NLRP3 and inhibits its oligomerization

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TR directly binds to NLRP3 and inhibits its oligomerization TR directly binds to NLRP3 and inhibits its oligomerization A, BCell lysates of LPS‐primed BMDMs (A) or PMA‐differentiated THP‐1. (B) Cells were incubated with different concentrations of biotin‐TR for 1 h, which were then pulled down with streptavidin beads.CPurified recombinant NLRP3 protein was incubated with different concentrations of biotin‐TR for 1 h, which were then pulled down with streptavidin beads.D, ECell lysates from HEK‐293T cells transfected with Flag‐tagged NLRP3, NOD1, NOD2, NLRP1, NLRC4, NLRP3‐LRR, NLPR3‐NACHT, or NLRP3‐PYD constructs were incubated with different concentrations of biotin‐TR for 1 h, which were then pulled down with streptavidin beads.FImmunoprecipitation (IP) and immunoblot analysis of the interaction of Flag‐NLRP3 and VSV‐NLRP3 in the lysates of HEK‐293T cells. TR was added at 8 h post‐transfection.GImmunoblot analysis of NLRP3 by SDD‐AGE or SDS–PAGE assay in BMDMs treated with TR for 30 min and then left stimulated with nigericin for 20 min.Data information: Data are representative of two or three independent experiments.Source data are available online for this figure. Yi Huang et al. EMBO Mol Med. 2018;emmm.201708689 © as stated in the article, figure or figure legend