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Group Name MRSA1 Group member name

Isolates Sample name Subtype Notes ERR070039-P11 ST2371 From Patient 11 ERR108054-P15 From Patient 15 ERR128707-P26 From Patient 26 ERR128718-HW From Health Care Worker - misses the ermC plasmid ERR070041-not-outbreak ST1 Not part of the outbreak

Result of species identification (KmerFinder) Strain Species acc. to Harris et al 2013 Predicted species Closest matching strain Template coverage ERR070039-P11 ERR108054-P15 ERR128707-P26 ERR128718-HW ERR070041-not-outbreak

Representative KmerFinder result

Result of Multilocus Sequence Typing (MLST) Strain ST acc. to Harris et al 2014 Predicted ST ERR070039-P11 ERR108054-P15 ERR128707-P26 ERR128718-HW ERR070041-not-outbreak

Representative Multilocus Sequence Typing result

Result of antibiotics resistance analysis Isolate Cefoxitin/Methicillin (MecA) Cipro-floxacin (chromo-somal mutation) Erythro-mycin (Erm(A), Erm(B), Erm(C)) Fusidic acid (chromo-somal mutation) Gentamicin (aac(6’)-aph(2'')) Mupirocin (chromo-somal mutation) Rifampicin (chromo-somal mutation) Tetra-cycline (tetC, tetM, tetR, tetS Vancomycin (Chromo-somal mutations or the vanA or vanB gene cluster.) Neomycin (aadD, aac(6')-aph(2'')) P11 P15 P26 HW non-out-break How is the agreement with the results presented in Harris et al 2013?

Result of plasmid analysis Isolate rep10 repUS12 rep20 rep5 rep16 rep7 ERR070039-P11 ERR108054-P15 ERR128707-P26 ERR128718-HW ERR070041-not-outbreak For each strain write which node the replicon was found on. If not found, leave the cell empty. Which plasmid is the ermC gene on?

Distance matrix made by NDtree and tree made with neighbour-joining

Distance matrix made by CSIPhylogeny and tree made with neighbour-joining