Overlap between changes in de novo protein synthesis after p53- or miR-34a-induction. Overlap between changes in de novo protein synthesis after p53- or.

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Overlap between changes in de novo protein synthesis after p53- or miR-34a-induction. Overlap between changes in de novo protein synthesis after p53- or miR-34a-induction.A, Left: Cumulative distribution plot displaying p53-induced changes in de novo protein synthesis of 113 proteins down-regulated after induction of miR-34a compared with the normal distribution of all detected proteins (28). Right: Venn diagram displaying the overlap between proteins down-regulated (log2 fold change ≤ −0.3) after ectopic p53 and miR-34a expression. B, Scatter plot displaying the correlation of changes in de novo protein synthesis between proteins down-regulated by p53 and miR-34a. The Pearson correlation coefficient and statistical significance are indicated. C, Enrichment of E2F1, E2F1DP1 and E2FDP2 binding motifs in the promoters of genes encoding proteins down-regulated by p53 and miR-34a. pSILAC data sets were analyzed with the Molecular Signature database (MSigDB) at http://www.broadinstitute.org/gsea/msigdb/index.jsp. TF = transcription factor. D, Cumulative distribution plot displaying the p53-induced changes in de novo protein synthesis of proteins with E2F1 binding motifs in their gene promoters (in black) compared with the normal distribution of all detected proteins (in light grey). p53-induced changes in de novo protein synthesis of proteins positively regulated by E2F1 are displayed in dark grey. E, Cumulative distribution plot displaying miR-34a-induced changes in de novo protein synthesis of proteins with E2F1 binding motifs in their gene promoters compared with the normal distribution of all detected proteins. The E2F1 data sets V$E2F_Q6 (141) and E2F1_UP.V1_UP (142) for (D) and V$E2F1_Q3_01 for (E) were obtained at MSigDB. Sabine Hünten et al. Mol Cell Proteomics 2015;14:2609-2629 © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.