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Volume 7, Issue 1, Pages 155-162 (July 1997) Bcl-2 Rescues T Lymphopoiesis, but Not B or NK Cell Development, in Common γ Chain–Deficient Mice Motonari Kondo, Koichi Akashi, Jos Domen, Kazuo Sugamura, Irving L Weissman Immunity Volume 7, Issue 1, Pages 155-162 (July 1997) DOI: 10.1016/S1074-7613(00)80518-X

Figure 1 Flow Cytometric Analysis of Nucleated Blood Cells in Each Mouse Strain (Top row) Expression of the transgene- derived human Bcl-2 protein. The Eμ-bcl-2 transgene was expressed predominantly in T cells (∼85% of T cells), whereas the H2K-bcl-2 transgene was expressed in virtually all nucleated cells in blood. (Bottom three rows) CD3/Gr-1, B220/Mac-1, and CD4/CD8 profiles in nucleated blood cells. Numbers accompanying inset boxes indicate the percentage of each population in nucleated blood cells. Immunity 1997 7, 155-162DOI: (10.1016/S1074-7613(00)80518-X)

Figure 2 Enforced Expression of Bcl-2 Restores Mature T Cells and Normalizes the Ratio of CD4+ to CD8+ T Cells in γc KO Mice (A) Absolute numbers (cells per microliter) of blood CD3+, B220+ and Gr-1+ cells. Asterisk, p < 0.05 by the Mann-Whitney and Wilcoxon nonparametric tests. (B) The ratio of CD3+CD4+ to CD3+CD8+ single-positive cells in each mouse strain. Open circles, γc KO mice; filled squares, Eμ-bcl-2 γc KO mice; filled circles, H2K-bcl-2 γc KO mice; open squares, control γc+ mice. Mean values are indicated by the bars and adjacent numbers. Immunity 1997 7, 155-162DOI: (10.1016/S1074-7613(00)80518-X)

Figure 3 Enforced Expression of Bcl-2 Restores Development of Thymocytes in γc KO Mice (A) CD4/CD8 profiles of total thymocytes in each mouse strain. Percentages of each subset (of total thymocytes) are also shown. (B) c-Kit/CD25 profiles of CD3− thymocyte populations. Percentages of each fraction (I–III) in CD3− thymocytes are indicated. Immunity 1997 7, 155-162DOI: (10.1016/S1074-7613(00)80518-X)

Figure 4 Enforced Expression of Bcl-2 Cannot Rescue B Cell Development in γc KO Mice Flow cytometric analysis of bone marrow B cell precursors in each mouse strain. (A) IgM/B220 profiles of total bone marrow cells. γc KO mice lack mature B220+IgM+ cells irrespective of the introduction of bcl-2 transgenes. (B) CD43/B220 profiles of IgM− population. The transition from the IgM−B220+CD43+ pro-B to the IgM−B220+CD43− pre-B stage was apparently impaired in γc KO strains. The numbers above the inset squares are the percentages of cells within the gate (of total bone marrow cells). (C) bcl-2 transgene expression in B220+IgM− B cell precursors in bone marrow of each bcl-2 transgenic γc KO strain (shaded histograms). Open histograms represent those of γc KO mice as negative controls. Virtually all B cell precursors in H2K-bcl-2 γc KO mice expressed human Bcl-2. Immunity 1997 7, 155-162DOI: (10.1016/S1074-7613(00)80518-X)

Figure 5 Enforced Expression of Bcl-2 Cannot Rescue NK Cell Development in γc KO Mice Flow cytometric analysis of nucleated cells in the spleen. Numbers accompanying inset boxes indicate the percentage of the gated population in splenocytes. NK1.1+CD3− NK cells could not be detected in either H2K-bcl-2 γc KO or γc KO mice. Immunity 1997 7, 155-162DOI: (10.1016/S1074-7613(00)80518-X)