Statistical evaluation of CD56+ NK cell subset data.

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binding sites 58 of the 473 unambiguously assigned phosphorylation sites are predicted by Scansite to be sites for binding. 50 of these correspond.

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Presentation transcript:

Statistical evaluation of CD56+ NK cell subset data. Statistical evaluation of CD56+ NK cell subset data.A, Box plots of protein regulation factors in CD56dim/bright and CD57+/− NK cell subsets. The box of each graph displays 50% of all determined protein regulation factors, the box height depicts their spreading and the black line within the boxes indicates the median. The restricting lines (high and low Whisker) display 1.5 × height of the box. All protein regulation factors above or beneath the Whiskers are categorized as strongly regulated and are shown as light dots. The symmetric distribution of protein regulations in all assessed donors with slight fluctuations is visible. Regulation ranges differ strongly within CD56dim/bright (between +3.8 and −4) and only slightly within CD57+/− NK cells (between +2.3 and −2.3), corresponding to their individual stage of differentiation. B, Heat maps of log2-protein regulation factors determined by iTRAQ-based LC-MS/MS from primary human CD56+ NK cell subsets. Heat maps were generated by loading NK cell subset-specific lists of proteins, identified in all five assessed human blood donors by iTRAQ-based LC-MS/MS, into the statistical program R (1162 in CD56dim/bright and 1427 within CD57+/− NK cells). Colored boxes in one row depict the regulation of one protein in five individual blood donors. One row depicts protein regulations in one blood donor. The histogram above displays the distribution of protein regulation factors and the corresponding color code. Maxi Scheiter et al. Mol Cell Proteomics 2013;12:1099-1114 © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.