Three different mimotope peptides mimic different domains of the the P berghei GAPDH molecule. Three different mimotope peptides mimic different domains.

Slides:

Advertisements

Similar presentations

Peptide reactivity between multiple sclerosis (MS) CSF IgG and recombinant antibodies generated from clonally expanded plasma cells in MS CSF Xiaoli.

Advertisements

Regulation and Destabilization of HIF-1α by ARD1-Mediated Acetylation

HDAC3, but not HDAC2 is in complex with tau protein.

Volume 55, Issue 1, Pages (July 2014)

Hirotaka Matsui, Hiroya Asou, Toshiya Inaba Molecular Cell

Human peptidoglycan recognition protein S is an effector of neutrophil-mediated innate immunity by Ju Hyun Cho, Iain P. Fraser, Koichi Fukase, Shoichi.

Hours after rec PR-Set7 release Ladder kDa 15 PR-Set7 Coomassie

Volume 22, Issue 3, Pages (May 2006)

Reduced copurification of selected co-opted host proteins with the viral replicase from yeast coexpressing WW-domain protein. Reduced copurification of.

Volume 76, Issue 1, Pages (July 2009)

by Subburaj Ilangumaran, Anne Briol, and Daniel C. Hoessli

Volume 11, Issue 6, Pages (June 2004)

Biochemical Mechanisms of IL-2–Regulated Fas-Mediated T Cell Apoptosis

Rose-Anne Romano, Barbara Birkaya, Satrajit Sinha

Involvement of Posttranslational Modification of NCAPP1 for Binding to Phloem Proteins.(A) Phosphorylation and glycosylation status of in planta–expressed.

UUKV NSs interacts with MAVS

Western blot of Aβ42-specific antibody compared with 4G8 antibody.

Yingqun Huang, Renata Gattoni, James Stévenin, Joan A. Steitz

LPA mRNA and Protein Expression Were Detected in Primarily Cultured HAVICs and Aortic Valve Tissues (A) RT-PCR detection of LPA mRNA expression in primarily.

NuMA directly interacts with Plk1 and gets phosphorylated at its C-terminus. NuMA directly interacts with Plk1 and gets phosphorylated at its C-terminus.

Efficient copurification of selected co-opted host proteins with the viral replicase from yeast coexpressing TPR-domain protein or cyclophilin A (CypA).

Identification of novel IgGs in alpaca serum.

Antibodies against PbGAPDH epitope peptides bind to sporozoite surface-GAPDH and inhibit sporozoite liver infection. Antibodies against PbGAPDH epitope.

Figure 3. MAb 19H9 displays broad cross-reactivity with IAV strains of different subtypes. (A), Amino acid sequence ... Figure 3. MAb 19H9 displays broad.

MED12 interacts with TDRD3 in a CARM1-dependent fashion.

Integrin α3β1-Dependent Activation of FAK/Src Regulates Rac1-Mediated Keratinocyte Polarization on Laminin-5 David P. Choma, Vincenzo Milano, Kevin M.

SYCE2 directly binds to the chromoshadow domain of HP1α.

Fine mapping assay with a G6 peptide library identifies two epitopes recognized by the anti-P39 antibody. Fine mapping assay with a G6 peptide library.

Yang Shen, Monica Naujokas, Morag Park, Keith Ireton Cell

Fig. 4. Identification of GRP78 as the target antigen of NMO-rAbs ON and ON Identification of GRP78 as the target antigen of NMO-rAbs.

Marina Cardó-Vila, Wadih Arap, Renata Pasqualini Molecular Cell

Yuji Yamanashi, David Baltimore Cell

Interaction of Cortactin and N-WASp with Arp2/3 Complex

Volume 25, Issue 5, Pages (November 2006)

Expression of EXP1-BirA

The PbGAPDH G6 fragment interacts with recombinant CD68.

Expression of SYCE2 inhibits the interaction of HP1α with H3K9me3.

Volume 91, Issue 4, Pages (November 1997)

FAK-Mediated Src Phosphorylation of Endophilin A2 Inhibits Endocytosis of MT1-MMP and Promotes ECM Degradation Xiaoyang Wu, Boyi Gan, Youngdong Yoo,

Andrei Kuzmichev, Thomas Jenuwein, Paul Tempst, Danny Reinberg

Adamtsl2 targeting strategy and validation of inactivation.

Volume 36, Issue 2, Pages (October 2009)

ACF can be tethered to a reporter locus through GAL4-DBD.

The histidine-rich loop regulates accessibility of the active site and RDEL motif in vivo. The histidine-rich loop regulates accessibility of the active.

Cross-reactivity of anti-Drosophila-synapsin with cockroach synapsin.

Fig. 2. Pull down of CaV2. 2 by channel C terminal fusion protein

Fig. 7. Ror-GFP binds to Myc-tagged Wnts and Wnt receptors.

Physical association of AP2, TPL and HDA19.

Limited subtilisin digestion of purified porcine brain tubulin.

Dissociation of HP1α from H3K9me3 is critical to potentiation of steady-state ATM activity. Dissociation of HP1α from H3K9me3 is critical to potentiation.

Domain organization of GAMA proteins and proteolytic processing of TgGAMA. Domain organization of GAMA proteins and proteolytic processing of TgGAMA. (A)

Salivary Lactoferrin Is Recognized by the Human Herpesvirus-8

A: Representative Western blot showing a band migrating at ∼90 kDa corresponding to neprilysin protein. A: Representative Western blot showing a band migrating.

Supershift assay of the Pax4 are shown.

GOLPH3 interacts with COG complex proteins.

Volume 55, Issue 1, Pages (July 2014)

DGKζ and Cbl-b deficient T Cells have enhanced ERK1/2 and IκBα phosphorylation. DGKζ and Cbl-b deficient T Cells have enhanced ERK1/2 and IκBα phosphorylation.

CXCR4 expression levels of MDA-MB-231 cells at 48 hours posttransfection of CXCR4 siRNAs. CXCR4 expression levels of MDA-MB-231 cells at 48 hours posttransfection.

Ectopic expression of CA RSK1 mutant protects melanoma cells from PD98059-mediated apoptosis. Ectopic expression of CA RSK1 mutant protects melanoma cells.

Functional consequences of the E1–KEAP1 interaction.

Interaction of Cdx1 and Cdx2 with the putative Cdx-binding sites of the human DSC2 promoter. Interaction of Cdx1 and Cdx2 with the putative Cdx-binding.

Pirh2 represses p73-dependent transactivation.

Identification of the recombinant structural proteins expressed in recombinant baculovirus-infected Sf9 cells by immunofluorescence assay with rabbit anti-FMDV.

Volume 91, Issue 4, Pages (November 1997)

Interaction with PRMT5 involves the RBD1,2 and RGG domains of nucleolin. Interaction with PRMT5 involves the RBD1,2 and RGG domains of nucleolin. A, schematic.

Induction of PARP cleavage (A) and activation of caspases (B) after treatment with a combination of TRAIL and cisplatin. Induction of PARP cleavage (A)

Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization in 231BR3 cells. Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization.

The interaction between PARsylated BRCA1 and RAP80 is required for maintaining BRCA1–RAP80–PARP1 complex integrity after DNA damage and normal HRR regulation.

Fig. 5 Dcr-2 directly binds to Toll 3′UTR.

RRC1 Interacts with phyB and Colocalizes in Nuclear Photobodies.

Presentation transcript:

Three different mimotope peptides mimic different domains of the the P berghei GAPDH molecule. Three different mimotope peptides mimic different domains of the the P berghei GAPDH molecule. (A) The anti-P39 antibody specifically recognizes PbGAPDH in Western blots of P berghei sporozoite lysates. Ponceau stain and the anti-actin antibody were used as loading controls. “S”: lysates of sporozoites purified from infected A stephensi salivary glands; “M”: mock lysates obtained from uninfected A stephensi salivary glands. Anti-CSP antibody served as a positive control for the sporozoite lysate. A polyclonal anti-PbGAPDH antibody (red arrow) identifies a sporozoite GAPDH band with identical mobility to the anti-P39 band. (B) Antibodies against each of the mimotope peptides (P39, P61, and P52) recognize bands with identical mobility as PbGAPDH (red arrow), but not the pET tag protein (blue arrow). Each panel shows two lanes, the left containing the pET tag protein alone and the right the tagged recombinant PbGAPDH protein. The antibodies used to probe the blots are indicated at the bottom of each panel. The anti-tag and anti-KLH antibodies served as a positive and negative controls, respectively. The anti-mouse GAPDH antibody was used as a positive control for identification of the GAPDH protein. The position of the recombinant protein and tag proteins is indicated by arrows to the right. All data are representative of two independent experiments. (C) Each anti-peptide antibody specifically recognizes its own peptide. The biotinylated peptide indicated at the top of each panel was bound to the wells of streptavidin-coated ELISA plates and tested for binding by the antibodies denoted at the bottom of each panel. No evidence of cross-reaction was detected. All data are representative of two independent assays. Sung-Jae Cha et al. LSA 2018;1:e201800111 © 2018 Cha et al.