Ana Cobo, Ph. D. , Aila Coello, Ph. D. , Jose Remohí, M. D

Slides:

Advertisements

Similar presentations

A time to look back: analysis of morphokinetic characteristics of human embryo development Javier Herrero, Ph.D., Alberto Tejera, Ph.D., Carmela Albert,

Advertisements

Krinos M. Trokoudes, M. D. , Constantinos Pavlides, M. Sc

Demián Glujovsky, M.D., M.Sc., Cynthia Farquhar, M.D., M.P.H.

Analysis of the morphological dynamics of blastocysts after vitrification/warming: defining new predictive variables of implantation Aila Coello, Ph.D.,

Study of nucleation status in the second cell cycle of human embryo and its impact on implantation rate Jesus Aguilar, Ph.D., Irene Rubio, Ph.D., Elkin.

A giant oocyte in a cohort of retrieved oocytes: does it have any effect on the in vitro fertilization cycle outcome? Ronit Machtinger, M.D., Joseph.

Report of results obtained in 2,934 women using donor sperm: donor insemination versus in vitro fertilization according to indication Thamara Viloria,

Adenomyosis does not affect implantation, but is associated with miscarriage in patients undergoing oocyte donation José A. Martínez-Conejero, Ph.D.,

Marcos Meseguer, Ph. D. , Rebeca Santiso, Ph. D. , Nicolas Garrido, Ph

Successful pregnancy and delivery of two sets of monozygotic twins after intracytoplasmic sperm injection and embryo transfer: case report and literature.

Samuel Santos-Ribeiro, M. D. , Johannie Siffain, M. D. , Nikolaos P

Morphokinetics as a predictor of self-correction to diploidy in tripronucleated intracytoplasmic sperm injection–derived human embryos Noelia Grau, Ph.D.,

Composition of single-step media used for human embryo culture

Vitrified-warmed blastocyst transfer cycles yield higher pregnancy and implantation rates compared with fresh blastocyst transfer cycles—time for a new.

Clinical application of oocyte vitrification: a systematic review and meta-analysis of randomized controlled trials Ana Cobo, Ph.D., César Diaz, M.D.

Zhi-Yong Yang, M.D., Ri-Cheng Chian, Ph.D. Fertility and Sterility

Marcos Meseguer, Ph. D. , Rebeca Santiso, Ph. D. , Nicolás Garrido, Ph

Time-dependent O2 consumption patterns determined optimal time ranges for selecting viable human embryos Alberto Tejera, Ph.D., Javier Herrero, Ph.D.,

Outcome of cryotransfer of embryos developed from vitrified oocytes: double vitrification has no impact on delivery rates Ana Cobo, Ph.D., Damià Castellò,

Live birth of twins derived from zona-free oocytes

New strategy for diagnosing embryo implantation potential by combining proteomics and time-lapse technologies Francisco Dominguez, Ph.D., Marcos Meseguer,

Management of the first in vitro fertilization cycle for unexplained infertility: a cost- effectiveness analysis of split in vitro fertilization-intracytoplasmic.

Effect of vitrification on human oocytes: a metabolic profiling study

Does the addition of time-lapse morphokinetics in the selection of embryos for transfer improve pregnancy rates? A randomized controlled trial Linnea.

The significance of sperm DNA oxidation in embryo development and reproductive outcome in an oocyte donation program: a new model to study a male infertility.

Improvements achieved in an oocyte donation program over a 10-year period: sequential increase in implantation and pregnancy rates and decrease in high-order.

Oocyte cryopreservation

Clinical validation of embryo culture and selection by morphokinetic analysis: a randomized, controlled trial of the EmbryoScope Irene Rubio, Ph.D.,

Embryo incubation and selection in a time-lapse monitoring system improves pregnancy outcome compared with a standard incubator: a retrospective cohort.

Sperm DNA fragmentation levels in testicular sperm samples from azoospermic males as assessed by the sperm chromatin dispersion (SCD) test Marcos Meseguer,

Autologous mitochondrial transfer as a complementary technique to intracytoplasmic sperm injection to improve embryo quality in patients undergoing in.

Automatic time-lapse instrument is superior to single-point morphology observation for selecting viable embryos: retrospective study in oocyte donation

Composition of commercial media used for human embryo culture

Optimizing the culture environment and embryo manipulation to help maintain embryo developmental potential Jason E. Swain, Ph.D., Doug Carrell, Ph.D.,

Reduced oxygen tension improves embryo quality but not clinical pregnancy rates: a randomized clinical study into ovum donation cycles Maria J. de los.

Kinetics of the early development of uniparental human haploid embryos

Type of chromosome abnormality affects embryo morphology dynamics

Embryo multinucleation at the two-cell stage is an independent predictor of intracytoplasmic sperm injection outcomes Laurent Desch, M.D., Céline Bruno,

Programming the endometrium for deferred transfer of cryopreserved embryos: hormone replacement versus modified natural cycles Eva R. Groenewoud, M.D.,

Morphokinetic analysis and embryonic prediction for blastocyst formation through an integrated time-lapse system Yamileth Motato, Ph.D., María José de.

Management of tubal ectopic pregnancy: methotrexate and salpingostomy are preferred to preserve fertility Stephanie Beall, M.D., Ph.D., Alan H. DeCherney,

Obesity reduces uterine receptivity: clinical experience from 9,587 first cycles of ovum donation with normal weight donors José Bellver, M.D., Antonio.

Normal birth after single-embryo transfer in a patient with excessive polypronuclear zygote formation following in vitro fertilization and intracytoplasmic.

Ana Cobo, Ph. D. , Juan A. García-Velasco, M. D. , Aila Coello, Ph. D

Age-specific probability of live birth with oocyte cryopreservation: an individual patient data meta-analysis Aylin Pelin Cil, M.D., Heejung Bang, Ph.D.,

Ongoing twin pregnancy after rescue intracytoplasmic sperm injection of unfertilized abnormal oocytes Navid Esfandiari, D.V.M., Ph.D., H.C.L.D., E. Anne.

Effect of semen quality on human sex ratio in in vitro fertilization and intracytoplasmic sperm injection: an analysis of 27,158 singleton infants born.

Value of the sperm deoxyribonucleic acid fragmentation level, as measured by the sperm chromatin dispersion test, in the outcome of in vitro fertilization.

Cumulative newborn rates increase with the total number of transferred embryos according to an analysis of 15,792 ovum donation cycles Nicolás Garrido,

Poor sperm quality affects clinical outcomes of intracytoplasmic sperm injection in fresh and subsequent frozen–thawed cycles: potential paternal effects.

Oocyte vitrification does not increase the risk of embryonic aneuploidy or diminish the implantation potential of blastocysts created after intracytoplasmic.

Kirstine Kirkegaard, M. D. , Johnny Juhl Hindkjaer, M. Sc

Storage of human oocytes in the vapor phase of nitrogen

Live babies born per oocyte retrieved in a subpopulation of oocyte donors with repetitive reproductive success J. Ryan Martin, M.D., Jason G. Bromer,

Ri-Cheng Chian, Ph.D., Peter S. Uzelac, M.D., Geeta Nargund, M.D.

Tong Du, M. S. , Hong Chen, M. D. , Rong Fu, M. S. , Qiuju Chen, Ph. D

José Bellver, M. D. , Yanira Ayllón, M. D. , Marcos Ferrando, M. D

Zsolt P. Nagy, M. D. , Ph. D. , Laura F. Rienzi, Ph. D. , Filippo M

Impact of different patterns of sperm chromosomal abnormalities on the chromosomal constitution of preimplantation embryos Lorena Rodrigo, B.Sc., Vanessa.

L. Scott, J. Bernsten, K. DeLegge, J. Hill, N. Ramsing

Sperm selection by swim-up in terms of deoxyribonucleic acid fragmentation as measured by the sperm chromatin dispersion test is altered in heavy smokers

Genetic evaluation procedures at sperm banks in the United States

Blastocyst biopsy with comprehensive chromosome screening and fresh embryo transfer significantly increases in vitro fertilization implantation and delivery.

David H. Barad, M.D., M.S., Norbert Gleicher, M.D.

Irene Rubio, Ph. D. , Reidun Kuhlmann, Inge Agerholm, Ph. D

Krinos M. Trokoudes, M. D. , Constantinos Pavlides, M. Sc

Influence of different oocyte insemination techniques on early and late morphokinetic parameters: retrospective analysis of 500 time-lapse monitored blastocysts

Effect of oocyte quality on blastocyst development after in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in a sheep model Maria.

Outcomes of vitrified early cleavage-stage and blastocyst-stage embryos in a cryopreservation program: evaluation of 3,150 warming cycles Ana Cobo, Ph.D.,

A time to look back: analysis of morphokinetic characteristics of human embryo development Javier Herrero, Ph.D., Alberto Tejera, Ph.D., Carmela Albert,

Presentation transcript:

Effect of oocyte vitrification on embryo quality: time-lapse analysis and morphokinetic evaluation Ana Cobo, Ph.D., Aila Coello, Ph.D., Jose Remohí, M.D., Jose Serrano, B.Sc.T., Jose Maria de los Santos, Ph.D., Marcos Meseguer, Ph.D. Fertility and Sterility Volume 108, Issue 3, Pages 491-497.e3 (September 2017) DOI: 10.1016/j.fertnstert.2017.06.024 Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Percentage of embryos falling within the optimal ranges proposed for the three variables included in the algorithm proposed by Meseguer et al. (2011): t5 (time to 5 cells), s2 (t4 − t3), and cc2 (t3 − t2), depending on oocyte origin (fresh vs. vitrified). In addition, exclusion criteria variables were included: direct-cleavage embryos (DC1–3), multinucleated embryos in the 4-cell stage (MN4), and embryos with uneven 1st cleavage (UN2). All proportions were significantly different between fresh and vitrified (P<.05). Fertility and Sterility 2017 108, 491-497.e3DOI: (10.1016/j.fertnstert.2017.06.024) Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 Morphokinetic decision tree algorithm used for embryo selection. t5 = time to division to 5 cells; cc2 = 2nd cell cycle, time from division to a 2-blastomere embryo to division to a 3-blastomere embryo (t3 − t2); s2 = 2nd synchrony, duration of the division transition from a 2-blastomere embryo to a 4-blastomere embryo (t4 − t3). The time of all events is expressed as hours after intracytoplasmic sperm injection. MN4 = multinucleated embryo in the 4-cell stage. Fertility and Sterility 2017 108, 491-497.e3DOI: (10.1016/j.fertnstert.2017.06.024) Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 2 Percentage of implanted embryos (implantation rate) depending on the embryo categories included in the algorithm proposed by Meseguer et al. (2011) and compared between fresh and vitrified oocytes. No statistical differences were observed in the implantation rates in each category depending on oocyte origin (P>.05). Fertility and Sterility 2017 108, 491-497.e3DOI: (10.1016/j.fertnstert.2017.06.024) Copyright © 2017 American Society for Reproductive Medicine Terms and Conditions