MY PLAN: Acquire reads Analyze reads Lengthen reads Find open reading frames Find proteins

Analyze Massive similarity of first 100 nt Need for editing

Lengthening read

Finding overlap within Making sure to always extend the ORF

Contig4-1 Length: 2751 nt ORF: 77-601 795-2108 2370-2702 Made up of 6 different reads

Open Reading Frame No hits compared to known viruses Only matches amino acids 8-126

Open Reading Frame No hits compared to known viruses Only matches amino acids 8-126

All Archea, Euryarchaeota Thermococcus sp. AM4 hyperthermophilic archaeon Thermococcus is extremely abundant in vent environments and this particular species and strain has the amazing ability to get its carbon and energy from carbon monoxide oxidation, resulting in hydrogen production. Iorn-sulfur protein

All Archea, Euryarchaeota Methanocaldococcus jannaschii DSM 2661 Methane-producing thermophilic archeon. This organism is an obligately anaerobic methane-producing archeon and was the first representative of the archaeal domain to be completely sequenced. Hypothetical protein

All Archea, Euryarchaeota Aciduliprofundum boonei T469 Widely distributed at deep-sea vents- only information has been in clone libraries. This is the first isolate, and it's sister lineage are the Thermplasmales Phosphoadenosine phosphosulfate reductase family protein reduction of PAPS to phospho-adenosine- phosphate (PAP)

Bacillus thuringiensis str. Al Hakam This organism, also known as BT, is famous for the production of an insecticidal toxin. This organism, like many other Bacilli, is found in the soil, integrase Integrase is an enzyme produced by a retrovirus that enables its genetic material to be integrated into the DNA of the infected cell.