The structure of AZT(3’-azido-2’,3’-dideoxythymidine)

Slides:

Advertisements

Similar presentations

Fundamentals of Biochemistry

Advertisements

Lecture 1 Problem: From an E. coli cell extract, you assay enzyme activity for beta-galactosidase. You divide the extract into two samples, one of which.

HIV and its lifecycle Sources: Wikipedia, HIV is a retrovirus (enveloped viruses possessing an RNA genome,

What Is New in HIV Infection? Journal of AAFP February 15, 2014 Volume 89, Number 4 Today is 3/26/14, and my name is Isaac Engholm, DO.

Treating HIV with Azidothymidine (AZT) A Design by Jeanine Nasser.

The gp41 fragment (purple) consists of a cytoplasmic tail and a hydrophobic membrane-spanning domain and is joined with the larger gp120 component (blue.

How do the Anti-HIV drugs woks? By Tawitch Suriyo Master degree of Toxicology Mahidol University.

Treatment of AIDS “Antiretroviral therapy & vaccines”

1 Antiviral Drugs Limited selective toxicity –Viruses mostly use host cell machinery, so very few unique targets –Most drugs block steps that take place.

Antivirals Slackers Facts by Mike Ori. Disclaimer The information represents my understanding only so errors and omissions are probably rampant. It has.

HIV Replication Rachel Carriger Biochemistry Fall 2004.

HIV and AIDS Human Immunodeficiency Virus (HIV) is the virus that causes Acquired Immunodeficiency Syndrome (AIDS).

Genetic Mutations Recombinant DNA Viruses Chapter 22 Nucleic Acids and Protein Synthesis.

Vaccines and Antivirals. Clinical Use of Interferon Therefore they have been used in the treatment of cancers of various types. Therefore they have been.

Antibiotics as nucleoside analouges

Larry Liu Professor Tal Lavian IEOR 190G Seminar-Patent (Burroughs-Wellcome) V.S.

Anti-HIV Drugs Cathy Molina November 11, Some HIV Facts HIV – the Human Immunodeficiency Virus is the retrovirus that causes AIDS HIV belongs to.

Combination of Drugs and Drug-Resistant Reverse Transcriptase Results in a Multiplicative Increase of Human Immunodeficiency Virus Type 1 Mutant Frequencies.

Antivirals for HIV Yasir Waheed, PhD. Some HIV Facts HIV – the Human Immunodeficiency Virus is the retrovirus that causes AIDS HIV belongs to the retrovirus.

1 Mukund Modak, Ph. D. Dental Biochemistry 2013 Lecture 39.

Biology and natural history of the virus

Antiviral Chemotherapy Discovery of antiviral drugs Targets of antiviral drugs.

Antiviral Agents Restricted spectrum

Chapter 5 Directions and Rates of Biochemical Processes.

Anti-HIV Drugs Melissa Morgan Medicinal Chemistry November 23, 2004.

From DNA to Protein. Knowledge of Nucleic Acid Chemistry Is Essential to the Understanding of DNA Structure.

Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display The Steps in HIV Replication Slide number 1 (1) Viral.

Nucleic Acids The nucleic acids DNA and RNA consist of monomers called nucleotides that consist of a Pentose sugar. Nitrogen-containing base. Phosphate.

Chapter 10 An Evolving Enemy Silvio Penta Silvio Penta Christie DiDonato Christie DiDonato Carl Tuoni Carl Tuoni Beth Miller Beth Miller.

Clinical case 19 Lin, I-Yao (Sally). Case 19 Having been confined in the hospital for almost a month due recurrent pneumonia, Mr. XXX, 42 y/o, married,

Medicines and Drugs Anti-virals Julia Barnes Anna Cruickshank.

Learning Objectives: Nucleic Acid therapeutics ReadingChapter 4, p from Blackburn & Gait Know the general mechanisms of the anti-cancer drugs.

Antiviral Drugs. Mechanism of Action Picornaviruses eg, polioviruses, cold viruses Block attachment molecule on host cell or pathogen Attachment antagonists.

Control of Gene Expression Year 13 Biology. Exceptions to the usual Protein Synthesis Some viruses contain RNA and no DNA. RNA is therefore replicated.

Prokaryotic Transcription Eukaryotic Transcription Both.

Retroviruses (Chap. 15, p.308) and Gene Regulation (Chap. 14) HIV (human immunodeficiency virus)

Using an 8 ½” x 11” sheet of paper, demonstrate your current understanding of TRANSCRIPTION by drawing the process in a manner that you understand and.

Polymerase Chain Reactions

The Nature of Viruses Chapter 27.

DNA REPLICATION. When the two strands of the DNA double helix are separated, each can serve as a template for the replication of a new complementary strand.

Antiviral drugs.

Fig. 5.3 Transforms R cells Fig. 5.5 Uracil (U) Five Fig. 5.7.

Basics of Human Immunodeficiency Virus (HIV) Brian Rybarczyk, PhD University of North Carolina- Chapel Hill.

Acyclovir Lee, sang-hwi.

General, Organic, and Biological Chemistry Copyright © 2010 Pearson Education, Inc Viruses Chapter 21 Nucleic Acids and Protein Synthesis.

Viro100: Virology 3 Credit hours NUST Centre of Virology & Immunology

Retrovirus HIV (humans) Animal viruses resulting in cancer / AIDS 15-39%

Virology – Antivirals 2 JU- 2 nd Year Medical Students By Dr Hamed AlZoubi – Microbiology and Immunology Department – Mutah University. MBBS (J.U.S.T)

Prokaryotic Transcription Eukaryotic Transcription Both.

Chapter 10 DNA Metabolism: Replication, Recombination, and Repair DNA polymerase & DNA Recombination.

DNA Replication the big event during S phase. The Animation hill.com/sites/ /student_view0/chapter14/animations.html#

Kalyan Das CABM & Rutgers University, NJ, USA

Create a concept map of the adaptive immune system.

Med Chem Tutoring January 25

Inhibitors of Transcription

S3 Fig Inactivity of inhibitors of reverse transcriptase and integrase

AIDS Chien-Ming Li MD, Ph.D.

OVERVIEW (continued) Figure 29.2

NUCLEIC ACIDS AS DRUG TARGETS

Evidence to support Darwin’s idea of “Descent with Modification”

Antiviral chemotherapy

Polymerase Chain Reaction

Of drug cocktails and inhibited enzymatic rates

Polymerases Polymerases are the enzymes that synthesize copies of nucleic acids. DNA polymerase I (DNA pol I) Reverse transcriptase (Rtase) the enzyme.

Translated By: Owais A. Shahzada

AZT = Azidothymidine (3’-azido-3’-deoxythymidine, Zidovudine)

Fingering the Ends Cell Volume 113, Issue 5, Pages (May 2003)

A Mechanism of AZT Resistance

Antiviral chemotherapy

Presentation transcript:

The structure of AZT(3’-azido-2’,3’-dideoxythymidine) The structure of AZT(3’-azido-2’,3’-dideoxythymidine).This nucleoside is a major drug in the treatment of AIDS.AZT is phosphorylated in vivo to give AZTTP(AZT 5’-triphosphate),a substrate analog that binds to HIV reverse transcriptase.HIV reverse transcriptase incorporates AZTTP into growing DNA chains in place of dTTP.Incorporated AZTMP blocks further chain elongation because its 3’azido group cannot form a phosphodiester bond with an incoming nucleotide.Host cell DNA polymerases have little affinity for AZTTP.