Skin test evaluation of a novel peptide carrier–based vaccine, BM32, in grass pollen– allergic patients Verena Niederberger, MD, Katharina Marth, MD,

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Presentation transcript:

Skin test evaluation of a novel peptide carrier–based vaccine, BM32, in grass pollen– allergic patients Verena Niederberger, MD, Katharina Marth, MD, Julia Eckl-Dorna, MD, PhD, Margarete Focke-Tejkl, PhD, Milena Weber, MSc, Wolfgang Hemmer, PhD, Uwe Berger, MBA, Angela Neubauer, PhD, Frank Stolz, PhD, Rainer Henning, PhD, Rudolf Valenta, MD Journal of Allergy and Clinical Immunology Volume 136, Issue 4, Pages 1101-1103.e8 (October 2015) DOI: 10.1016/j.jaci.2015.03.034 Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Comparison of immediate SPT reactions to grass pollen extract, BM32, and individual BM32 components. Mean wheal areas (mm2, y-axis) induced with codein phosphate, grass pollen extract, and 3 concentrations (11, 33, 100 μg/mL) of BM32, BM321, BM322, BM325, and BM326 (x-axis) in the 60 study participants are displayed. Horizontal bars denote medians. The dotted line indicates the cutoff. Calculation: (1.5 mm2 × π) = 7.07 mm2. Journal of Allergy and Clinical Immunology 2015 136, 1101-1103.e8DOI: (10.1016/j.jaci.2015.03.034) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 Schematic representation of BM32 components BM321, BM322, BM325, and BM326 showing the sequences of the allergen-derived peptides and their position in the PreS fusion proteins. Journal of Allergy and Clinical Immunology 2015 136, 1101-1103.e8DOI: (10.1016/j.jaci.2015.03.034) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 Exposure of study participants to grass pollen during the study. The x-axis displays the time of grass pollen exposure in Vienna from April 1 to August 31. The y-axis shows pollen counts (gray line, grains per m3) as well as the number of subjects enrolled at the respective time points (red columns). Journal of Allergy and Clinical Immunology 2015 136, 1101-1103.e8DOI: (10.1016/j.jaci.2015.03.034) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E3 Comparison of IgE reactivities to recombinant allergens and BM32 components in the study population. Shown are IgE levels (y-axis, OD values) specific for each of the allergens and the corresponding BM32 components (x-axis). Mean IgE levels are indicated by horizontal bars. Journal of Allergy and Clinical Immunology 2015 136, 1101-1103.e8DOI: (10.1016/j.jaci.2015.03.034) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E4 Correlation of timothy grass pollen allergen-specific IgE with recombinant allergen-specific IgE in the study population. Shown are IgE levels to timothy grass pollen extract (x-axis) and the sums of IgE levels to Phl p 1, Phl p 2, Phl p 5, and Phl p 6 (y-axis) in kUA/L. Journal of Allergy and Clinical Immunology 2015 136, 1101-1103.e8DOI: (10.1016/j.jaci.2015.03.034) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions