Molecular and immunohistochemical detection of rotavirus in urinary sediment cells of children with rotavirus gastroenteritis T. Yokoyama, N. Sugimoto,

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Presentation transcript:

Molecular and immunohistochemical detection of rotavirus in urinary sediment cells of children with rotavirus gastroenteritis T. Yokoyama, N. Sugimoto, K. Taniguchi, S. Komoto, T. Yuno, K. Ohta, H. Hashimoto, A. Seno, A. Ashida, M. Fujieda, S. Nishio, K. Ueno, M. Shimizu, A. Yachie Clinical Microbiology and Infection Volume 17, Issue 8, Pages 1190-1193 (August 2011) DOI: 10.1111/j.1469-0691.2011.03522.x Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Detection of rotaviral antigens and double-stranded RNA in urinary sediments of rotavirus (RV)-infected patients. (a) Renal tubular cell-shaped inclusion-bearing cell. (b) Immunofluorescent staining of renal tubular cells: a cytokeratin-positive cell (fluorescein isothiocyanate: green) coexpressing RV antigen (rhodamine: red); a double-positive cell with orange cytoplasm (merged: orange). The cells in the lower box are RV-infected MA104 cells (positive controls). (c) Results of nested RT-PCR of urinary sediments. Four of five RV-infected patients showed RV RNA. The stool of an RV-infected patient was used as a positive control. The urine from a non-RV gastroenteritis patient and an IgA nephropathy (IgAN) patient were used as negative controls. Clinical Microbiology and Infection 2011 17, 1190-1193DOI: (10.1111/j.1469-0691.2011.03522.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions