Bitter taste receptor (TAS2R) agonists inhibit IgE-dependent mast cell activation Maria Ekoff, PhD, Jeong-Hee Choi, MD, PhD, Anna James, PhD, Barbro Dahlén,

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Presentation transcript:

Bitter taste receptor (TAS2R) agonists inhibit IgE-dependent mast cell activation Maria Ekoff, PhD, Jeong-Hee Choi, MD, PhD, Anna James, PhD, Barbro Dahlén, MD, PhD, Gunnar Nilsson, PhD, Sven-Erik Dahlén, MD, PhD Journal of Allergy and Clinical Immunology Volume 134, Issue 2, Pages 475-478 (August 2014) DOI: 10.1016/j.jaci.2014.02.029 Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 A, TAS2R mRNA expression of CBMCs and the human mast cell line HMC-1.2, measured with real-time PCR (N = 3). TaqMan primers for human TAS2Rs and GAPDH, ACTB, and HPRT-1 were used. Gene expression was calculated according to the 2−ΔCT formula, and expressed as follows: 2ˆ-(receptor cyclic threshold [CT] minus average CT of all housekeeping genes) × 103. B and C, TAS2R agonists (60-minute incubation) do not induce release of histamine or PGD2 in CBMCs. Journal of Allergy and Clinical Immunology 2014 134, 475-478DOI: (10.1016/j.jaci.2014.02.029) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 TAS2R agonists inhibit histamine and/or PGD2 release after IgECL of CBMCs (N = 6-8). Cells were incubated with 1 μg/mL IgE (Calbiochem) overnight and agonists were added 30 minutes before activation with 2 μg/mL α-IgE (Sigma). A and B, The effect of chloroquine on release. C and D, The effect of denatonium on release. E and F, The effect of dextromethorphan on release. G and H, The effect of noscapine on release. Journal of Allergy and Clinical Immunology 2014 134, 475-478DOI: (10.1016/j.jaci.2014.02.029) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions