Supplemental Figure Secreted PCSK9 (ng/ml)

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Supplemental Figure 1 350 300 Secreted PCSK9 (ng/ml) 250 200 150 100 50 100 150 200 250 300 350 Secreted PCSK9 (ng/ml) WT V5 QH V5 ng Input DNA 125 ng non-relevant DNA 375 WT NT Input DNA 500 ELISA demostrating the linearity of WT PCSK9 Secretion from HuH7 cells transfected with WT PCSK9 untagged (WT NT) and V5-tagged WT PCSK9 (WT V5) but no secretion with Q152H PCSK9 (QH V5), with amounts of cDNA as shown. n=3; ave  SEM. HuH7 cells were grown at 37C in DMEM + 10% FBS + gentamycin (28 g/ml). Cells (3X105) were transiently-transfected with cDNA encoding human PCSK9 (pIRES2) using Effectene protocol from Qiagen 24 hours following plating.