Optimal ratio of adipose stem cells and bone marrow stem cell to promote osteogenic differentiation and angiogenesis G.-I. Im, K.-I. Kim, N.-H. Jo Osteoarthritis.

Slides:

Advertisements

Similar presentations

Full-thickness cartilage defects repaired by human induced pluripotent stem cells (IPSCS)-derived mesenchymal stem cells and plga scaffolds D. Shi, X.

Advertisements

M. M. J. Caron, P. J. Emans, M. M. E. Coolsen, L. Voss, D. A. M

Do adipose tissue-derived mesenchymal stem cells have the same osteogenic and chondrogenic potential as bone marrow-derived cells? Gun-II Im, M.D., Yong-Woon.

Muscle cell-derived factors inhibit inflammatory stimuli-induced damage in hMSC- derived chondrocytes R.S. Rainbow, H. Kwon, A.T. Foote, R.C. Preda, D.L.

Full-thickness cartilage defects repaired by human induced pluripotent stem cells (IPSCS)-derived mesenchymal stem cells and plga scaffolds D. Shi, X.

D. N. Clements, B. Sc. , B. V. Sc. , S. D. Carter, Ph. D. , J. F

M. M. J. Caron, P. J. Emans, M. M. E. Coolsen, L. Voss, D. A. M

Z. Zhang, Y. Kang, Z. Zhang, H. Zhang, X. Duan, J. Liu, X. Li, W. Liao

Hypertrophic differentiation during chondrogenic differentiation of progenitor cells is stimulated by BMP-2 but suppressed by BMP-7 M.M.J. Caron, P.J.

Adipose-derived stem cells induce autophagic activation and inhibit catabolic response to pro-inflammatory cytokines in rat chondrocytes Li-Bo Jiang,

Immunomodulatory effect of mesenchymal stem cells following intra-articular injection in a model of osteoarthritis: a potential role for apoptosis P.

Subtractive gene expression profiling of articular cartilage and mesenchymal stem cells: serpins as cartilage-relevant differentiation markers S. Boeuf,

K.A. Payne, D.M. Didiano, C.R. Chu Osteoarthritis and Cartilage

C.B. Chang, S.A. Han, E.M. Kim, S. Lee, S.C. Seong, M.C. Lee

G.-I. Im, H.-J. Kim Osteoarthritis and Cartilage

C.B. Chang, S.A. Han, E.M. Kim, S. Lee, S.C. Seong, M.C. Lee

S. Gavard Osteoarthritis and Cartilage

Gene expression profiling for confirmation of effects of DNA polymeric molecules on in vitro arthritis model H.-J. Kim, A. Baek, M. Kim, S.-R. Cho Osteoarthritis.

In vitro cell surface markers are insufficient to identify in vivo/in situ multipotent synovial mesenchymal stem cells isolated from normal or osteoarthritic.

Exaggerated inflammatory environment decreases BMP-2/ACS-induced ectopic bone mass in a rat model: implications for clinical use of BMP-2 R.-L. Huang,

Differential expression of interleukin-17 and interleukin-22 in inflamed and non-inflamed synovium from osteoarthritis patients C. Deligne, S. Casulli,

Prospective economic evaluation of the costs for medications and nutraceuticals in knee osteoarthritis M. Chung, N. Zhao, J.B. Wong, C. Wang Osteoarthritis.

Reduced chondrocyte proliferation, earlier cell cycle exit and increased apoptosis in neuronal nitric oxide synthase-deficient mice Q. Yan, Q. Feng,

Proinflammatory cytokines inhibit osteogenic differentiation from stem cells: implications for bone repair during inflammation D.C. Lacey, P.J. Simmons,

Osteoarthritis year 2011 in review: biochemical markers of osteoarthritis: an overview of research and initiatives Y. Henrotin Osteoarthritis and Cartilage

Decreased proteoglycan degradation in IL-1β-treated cartilage co-cultured with TIMP-3- transduced cells J. Mason, A. Donahue, A. Yoskowitz, D. Richardson

Quantitatively-measured bone marrow lesions in the patellofemoral joint: distribution and association with pain C. Ratzlaff, R. Russell, J. Duryea Osteoarthritis.

Role of endothelin-1 in endochondral ossification and osteoarthritis

Automatic cartilage segmentation and measurement for diagnosis of OA using 3D box and Gaussian filters X. Cui, S. Li, M. Yu, H. Kim, K.-S. Kwack, B.-H.

Transcription factor SPB-x is a key molecule inducing hypertrophy of differentiated chondrocyte from MSC G.-I. Im, J.-M. Lee, J.-M. Ahn, E.-A. Kim Osteoarthritis.

Effect of self assembled peptide-mesenchymal stem cell complex on delaying the progression of osteoarthritis S. Kim, S. Lee, Y. Jung, J. Kim, S. Kim

Expression of the semicarbazide-sensitive amine oxidase in articular cartilage: its role in terminal differentiation of chondrocytes in rat and human

Multifunctional nanoparticles for improving function and multimodal tracking of mesenchymal stem cells for osteoarthritis treatment I.M. Adjei, H. Yang,

Effect of mechanical stress on the hypertrophic differentiation related gene expression in cultured chondrocytes H. Inoue, Y. Arai, R. Terauchi, S. Nakagawa,

Z. Zhang, W. Liao, Z. Zhang, X. Duan, C. Hou

The differences on extracellular matrix among each portion of meniscus

Immunomodulatory effect of mesenchymal stem cells following intra-articular injection in a model of osteoarthritis: a potential role for apoptosis P.

Heterozygosity for an inactivating mutation in low-density lipoprotein-related receptor 6 (Lrp6) increases osteoarthritis severity in mice after ligament.

Time to be positive about negative data?

Osteoarthritis and Cartilage

Y. Zhou, L. Resutek, L. Wang, X. Lu Osteoarthritis and Cartilage

Enhancing and maintaining chondrogenesis of synovial fibroblasts by cartilage extracellular matrix protein matrilins M. Pei, M.D., Ph.D., J. Luo, M.D.,

M. M. J. Caron, P. J. Emans, M. M. E. Coolsen, L. Voss, D. A. M

Stem cell therapy for human cartilage defects: a systematic review

Undifferentiated human mesenchymal stem cells (hMSCs) are highly sensitive to mechanical strain: transcriptionally controlled early osteo-chondrogenic.

N.D. Miljkovic, M.D., Ph.D., G.M. Cooper, Ph.D., K.G. Marra, Ph.D.

B. Vafaeian, S. Adeeb, M. El-Rich, J. Jaremko

M. A. Cleary, R. Narcisi, K. Focke, R. van der Linden, P. A. J

Assessing the characteristics of human mesenchymal stromal cells derived from bone marrow and umbilical cord following expansion in the quantum® hollow-fibre.

Role of TCF4 in signaling crosstalk with NFκB in human chondrocytes

Inducible Expression of Viral Interleukin 10 in Mouse Mesenchymal Stem Cells Increases their Immuno-suppressive Capacity S. Raman, P. Mancuso, A. Ryan,

Bisphosphonate rescues cartilage from trauma damage by promoting mechanical sensitivity and calcium signaling in chondrocytes Y. Zhou, M. Park, L. Wang,

Different responses of healthy, relative healthy and OA chondrocytes with IL1β treatment under hypoxia and normoxia X. Huang, L. Zhong, J. Post, M. Karperien

Cell surface receptor expression profile of human synovial mesenchymal stem cells in- vivo predicts their differentiation potential in-vitro N.U. Aljezani,

Do adipose tissue-derived mesenchymal stem cells have the same osteogenic and chondrogenic potential as bone marrow-derived cells? Gun-II Im, M.D., Yong-Woon.

Emerging role of endothelin receptor type B in regulating chondrogenic and hypertrophic changes of human mesenchymal stem cells M. Au, M. Zhu, W.-J.

Z. Huang, T. Stabler, F. Pei, V. Kraus Osteoarthritis and Cartilage

A peptide temporally enhanced chondrogenesis of mesenchymal stem cells

Evidence for bone mineral density and bone resorption in middle and elderly women with knee osteoarthritis in Shanghai: a cross sectional study Q. Xiaofeng

Novel juvenile factors for cartilage regeneration

Location-specific hip joint space width for progression of hip osteoarthritis: predictive validity and responsiveness of a new computerized measure C.

Imaging biomarker with T1 and T2 mappings in osteoarthritis – In vivo human articular cartilage study C. Yan, C. Wong, T. Li, N. Gong, Q. Chan, K. Chiu

How does lower leg alignment differ between soccer players, other athletes and non- athletic controls? W. colyn, R. Agricola, N. Arnout, J. Verhaar, J.

Hip derived synovial mesenchymal progenitor cell surface markers as indicators for differentiation potential A. Affan, N. Al-Jezani, P. Railton, J. Powell,

A. B. González-Gil, E. Muinos-López, P. Ripalda-Cemboráin, T

Adhesive barrier/directional controlled release for cartilage repair by endogenous progenitor cell recruitment G.-I. Im, J.-M. Lee, E.-A. Kim Osteoarthritis.

K. -C. Wang, E. Kwan, K. Aris, T. T. Egelhoff, A. I. Caplan, J. F

Structured three-dimensional co-culture of mesenchymal stem cells with chondrocytes promotes chondrogenic differentiation without hypertrophy M.E. Cooke,

General Information Osteoarthritis and Cartilage

Long-term effects of vitamin d supplementation and maintaining vitamin d sufficiency on knee osteoarthritis over 5 years B. Antony, S. Zheng, J. Burgess,

Presentation transcript:

Optimal ratio of adipose stem cells and bone marrow stem cell to promote osteogenic differentiation and angiogenesis G.-I. Im, K.-I. Kim, N.-H. Jo Osteoarthritis and Cartilage Volume 22, Pages S129-S130 (April 2014) DOI: 10.1016/j.joca.2014.02.237 Copyright © 2014 Terms and Conditions

Fig. 1 Effects of ASCs cultured in transwell on BMSCs. ASC 0.25, 0.50, 0.75, I means that 0.25 × 105, 0.5 × 105, 0.75 × 105, and 1.0 × 105 ASCs respectively were cultured in a transwell insert over 105 BMSCs on the lower well. (A) ALP staining, quantification of ALP activity and the ALP mRNA expression from RT-qPCR after 14 days. (B) Von Kossa staining, quantification of calcium contents after 14 days. All values are shown as percentages over the control (BMSCs without ASCs). Bar represents mean ± SD, p < 0.05: * significantly greater than the control; # significantly lower than the control. Osteoarthritis and Cartilage 2014 22, S129-S130DOI: (10.1016/j.joca.2014.02.237) Copyright © 2014 Terms and Conditions

Fig. 2 Effects of ASCs in mixed coculture with BMSCs. The number of BMSCs is fixed at 1.0 × 105. “ASC : BMSC = 0.25: 1” means that 0.25 × 105 ASCs were cocultured with 1.0 × 105 BMSCs. All values are shown as percentages over the control (BMSCs without ASCs). (a) ALP activity (b) Calcium contents (c) RT-qPCR for osteogenic markers (d) ELISA for osteogenic markers after 14 days. Bar represents mean ± SD. N = 3 for all panels. p < 0.05, *significantly greater than the control; # significantly lower than the control. Osteoarthritis and Cartilage 2014 22, S129-S130DOI: (10.1016/j.joca.2014.02.237) Copyright © 2014 Terms and Conditions