Dog peritoneal and pleural cavities as bioreactors to grow autologous vascular grafts Wai-Leng Chue, MBBS, Gordon R Campbell, PhD, Noel Caplice, MBBS,

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Presentation transcript:

Dog peritoneal and pleural cavities as bioreactors to grow autologous vascular grafts Wai-Leng Chue, MBBS, Gordon R Campbell, PhD, Noel Caplice, MBBS, PhD, Amjid Muhammed, BSc, Celia L Berry, BSc, Anita C Thomas, MSc, Michael B Bennett, PhD, Julie H Campbell, PhD Journal of Vascular Surgery Volume 39, Issue 4, Pages 859-867 (April 2004) DOI: 10.1016/j.jvs.2003.03.003

Fig 1 A, Capsule of avascular tissue that has grown around a piece of polyethylene tubing 80 mm long, with 4.75-mm external diameter, placed in the peritoneal cavity of a dog for 3 weeks. Thickness of the wall of tissue is about 1 mm. B, Capsule of tissue about 20 cm long, grown in the dog peritoneal cavity for 3 weeks, with inner polyethylene tubing removed. Journal of Vascular Surgery 2004 39, 859-867DOI: (10.1016/j.jvs.2003.03.003)

Fig 2 Sections of a capsule of tissue grown around a length of polyethylene tubing in the peritoneal cavity of the dog for 3 weeks. A, Stained with hematoxylin-eosin. B, Immunostained for α-smooth muscle actin. C, Immunostained for desmin. D, Immunostained for CD45. Journal of Vascular Surgery 2004 39, 859-867DOI: (10.1016/j.jvs.2003.03.003)

Fig 3 A, Central region of a tube of tissue that had grown in the peritoneal cavity of a dog for 3 weeks, 6.5 months after it had been grafted into the femoral artery in the same dog. B, Same graft, but at the region of the proximal anastomosis. Arrow, suture. Journal of Vascular Surgery 2004 39, 859-867DOI: (10.1016/j.jvs.2003.03.003)

Fig 4 Immunostaining of a tube of tissue grown in the peritoneal cavity of a dog for 3 weeks, then grafted into the femoral artery of the same dog for 4 months. A, Smooth muscle myosin. B, Vimentin. C, Desmin. D, Smoothelin. Journal of Vascular Surgery 2004 39, 859-867DOI: (10.1016/j.jvs.2003.03.003)

Fig 5 Elongated spindle-shaped “smooth muscle cell” from a tube of tissue grown in the peritoneal cavity of a dog for 3 weeks, then grafted into the femoral artery of the same dog for 6.5 months. A, Basal lamina and collagen bundles orientated in different directions surround the cell. Bar = 1 μm. B, Higher magnification of a smooth muscle cell from the same graft as in A demonstrates the presence of an extensive cytoplasmic network of myofilaments with associated dark bodies. A lipid droplet, mitochondria, and rough endoplasmic reticulum are also observed. A thick floccular basal lamina and collagen fibers surround the cell. Bar = 200 nm. Journal of Vascular Surgery 2004 39, 859-867DOI: (10.1016/j.jvs.2003.03.003)

Fig 6 Cells lining the lumen of a tube of tissue grown in the peritoneal cavity of a dog for 3 weeks, then grafted into the femoral artery of the same dog for 4 months. A, Stained with toluidine blue. B, Immunostained for Ulex lectin. C, Scanning electron microscopic view. Journal of Vascular Surgery 2004 39, 859-867DOI: (10.1016/j.jvs.2003.03.003)