Neutrophils contain bacteria inside the phagolysosome for more than 2 days. Neutrophils contain bacteria inside the phagolysosome for more than 2 days.

Slides:

Advertisements

Similar presentations

Molecular Therapy - Oncolytics

Advertisements

by Carmen W. van den Berg, and Karolina E. Taylor

Acalabrutinib and ibrutinib therapy cause dysfunctional GPVI-mediated platelet aggregation. Acalabrutinib and ibrutinib therapy cause dysfunctional GPVI-mediated.

by Kathryn Lagrue, Alex Carisey, David J

by Gail Dallalio, Erin Law, and Robert T. Means

RGFP-966 decreases HDAC3 activity and is not cytotoxic.

Cytosolic pH and the inflammatory microenvironment modulate cell death in human neutrophils after phagocytosis by Raymond J. Coakley, Clifford Taggart,

Exposure to fluid shear stress modulates the ability of endothelial cells to recruit neutrophils in response to tumor necrosis factor-α: a basis for local.

Metalloproteinases Are Involved in Lipopolysaccharide– and Tumor Necrosis Factor-–Mediated Regulation of CXCR1 and CXCR2 Chemokine Receptor Expression.

Macrophages from C3-deficient mice have impaired potency to stimulate alloreactive T cells by Wuding Zhou, Hetal Patel, Ke Li, Qi Peng, Marie-Bernadette.

Sex differences in resident immune cell phenotype underlie more efficient acute inflammatory responses in female mice by Ramona S. Scotland, Melanie J.

Interleukin-21 is a growth and survival factor for human myeloma cells

Serum amyloid A is an innate immune opsonin for Gram-negative bacteria

Oxidative Stress–Induced Calreticulin Expression and Translocation: New Insights into the Destruction of Melanocytes Yajun Zhang, Ling Liu, Liang Jin,

The heat shock protein Gp96 binds to human neutrophils and monocytes and stimulates effector functions by Markus P. Radsak, Norbert Hilf, Harpreet Singh-Jasuja,

Transfer of miR-223 during neutrophil-epithelial cell interactions

Functional human regulatory T cells fail to control autoimmune inflammation due to PKB/c-akt hyperactivation in effector cells by Ellen J. Wehrens, Gerdien.

by Beatriz Bellosillo, Mireia Dalmau, Dolors Colomer, and Joan Gil

CD11c+ dendritic cells and plasmacytoid DCs are activated by human cytomegalovirus and retain efficient T cell-stimulatory capability upon infection by.

by Eleanor J. Molloy, Amanda J. O'Neill, Julie J

Fig. 4. aPD-1 mAb transfer to macrophages is mediated by FcγRs.

Mature murine megakaryocytes present antigen-MHC class I molecules to T cells and transfer them to platelets by Anne Zufferey, Edwin R. Speck, Kellie R.

Inter-α inhibitor proteins maintain neutrophils in a resting state by regulating shape and reducing ROS production by Soe Soe Htwe, Hidenori Wake, Keyue.

Crosstalk between ROR1 and BCR pathways defines novel treatment strategies in mantle cell lymphoma by Hanna Karvonen, David Chiron, Wilhelmiina Niininen,

A, Phase-contrast and CSLM of Arabidopsis roots cocultured with the B

TCF1 expression marks self-renewing human CD8+ T cells

by Cheryl L. Maier, Amanda Mener, Seema R. Patel, Ryan P

WNT ligands contribute to the immune response during septic shock and amplify endotoxemia-driven inflammation in mice by Marcela Gatica-Andrades, Dimitrios.

Jeffrey M. Rhodes, MD, Anna F. Tilberg, MS, Robert R.M. Gifford, MD

LV pseudotransduction delivers proteins and activates DCs.

Differential antibacterial control by neutrophil subsets

Severe platelet dysfunction in NHL patients receiving ibrutinib is absent in patients receiving acalabrutinib by Alexander P. Bye, Amanda J. Unsworth,

BRAFV600E promotes glycolysis in melanoma cells via regulation of GLUT1, GLUT3, and HK2. BRAFV600E promotes glycolysis in melanoma cells via regulation.

Defective negative regulation of Toll-like receptor signaling leads to excessive TNF-α in myeloproliferative neoplasm by Hew Yeng Lai, Stefan A. Brooks,

Fig. 2. IL-2/rapamycin–expanded T cells express homing receptors to traffic to lymphoma sites and are resistant to SN-38 toxicity. IL-2/rapamycin–expanded.

Reduction of neutrophil airspace influx does not significantly attenuate alveolar barrier disruption in thrombocytopenic Mpl−/− mice following either live.

by Defne Bayik, Debra Tross, Lydia A

by Nicholas J. Laping, Michael P. DeMartino, Joshua E

GlyR activation modulates its diffusion properties around active synapses. GlyR activation modulates its diffusion properties around active synapses. A,

Human hematopoietic stem cell maintenance and myeloid cell development in next-generation humanized mouse models by Trisha R. Sippel, Stefan Radtke, Tayla.

Effect of expression of constitutively active CAMKK2 on AMPK activation and the cell cycle in G361 cells. Effect of expression of constitutively active.

CSF1 secretion by melanoma cells is induced by CTL-derived cytokines

Relative growth of WT R2866 and the R2866ΔampG gene deletion mutant with Triton X-100. Relative growth of WT R2866 and the R2866ΔampG gene deletion mutant.

TriKE–treated NK cells overcome MDSC-induced immune suppression.

Volume 60, Issue 4, Pages (October 2001)

AML cells display differential sensitivity to inhibition of IKBKE and TBK1. AML cells display differential sensitivity to inhibition of IKBKE and TBK1.

Comparison of male and female CD8+ T cells primed with different amounts of cognate Ag. (A) Schematic of experimental design: 1 × 104 gBT-I CD8+ T cells.

Fig. 1 KCC2 down-regulation is prevented in sortilin-deficient mice.

Fig. 4 Labeling of Msmeg with DMN-Tre is fast and specific and depends on Ag85A function. Labeling of Msmeg with DMN-Tre is fast and specific and depends.

Correlation between CCL26 production by human bronchial epithelial cells and airway eosinophils: Involvement in patients with severe eosinophilic asthma

IL-33, IL-25, and TSLP induce a distinct phenotypic and activation profile in human type 2 innate lymphoid cells by Ana Camelo, Guglielmo Rosignoli, Yoichiro.

Fig. 6 Functional characterization of RdRp activity of the recMeV mutants with modified N proteins. Functional characterization of RdRp activity of the.

Senescence-associated defective HLA-DR upregulation does not modulate immunosuppressive properties of MSCs. (A) Fit and senescent MSCs were subjected to.

CPPED1 (A) and PPARγ2 (B) mRNA expressions in cultured SGBS cells during adipocyte differentiation. CPPED1 (A) and PPARγ2 (B) mRNA expressions in cultured.

Shared phenotype of CD4+CLA+CD103+ T cells from human blood and skin.

Src kinases mediate TSP-1 inhibition of AC and PKA activity.

Blood Tfr cells do not show specialized humoral regulatory capacity.

by Martin Felices, Behiye Kodal, Peter Hinderlie, Michael F

Targeting CD47 in Sézary syndrome with SIRPαFc

Peripheral surgery regulates expression of astrocytic markers.

Granulocyte colony-stimulating factor mobilizes dormant hematopoietic stem cells without proliferation in mice by Jeffrey M. Bernitz, Michael G. Daniel,

by Geoffrey A. Smith, Jack Taunton, and Arthur Weiss

Fig. 5 Increased myometrial cell contractility in response to fetal T cells from preterm infants. Increased myometrial cell contractility in response to.

Patient Tregs express normal levels of suppression.

Trametinib and combination promote moDC maturation and decrease moDC viability. Trametinib and combination promote moDC maturation and decrease moDC viability.

Human basophils are unresponsive to contact-dependent or contact-independent inhibition by Tregs. Human basophils are unresponsive to contact-dependent.

CpG oligodeoxynucleotide (CpG ODN)–induced activation of different types of primary malignant B cells. CpG oligodeoxynucleotide (CpG ODN)–induced activation.

Fig. 2 BX795 is nontoxic to HCE cells at therapeutic concentration.

Fig. 1 Induction of IgM and IgG following controlled human P

Effect of inhibition of phagocytosis on the production of reactive oxygen species (ROS) against Aspergillus fumigatus. Effect of inhibition of phagocytosis.

Presentation transcript:

Neutrophils contain bacteria inside the phagolysosome for more than 2 days. Neutrophils contain bacteria inside the phagolysosome for more than 2 days. (A) Coculture of neutrophils and SA-GFP in fibrin gels. Increasing concentrations of neutrophils from healthy controls were incubated with a fixed amount of bacteria (5 × 106 CFUs per mL) (representative data; n = 20). Symbols represent the mean of duplicates. Bacterial proliferation is expressed in GFP-relative fluorescence units (RFU). (B) TUO of SA-GFP (5 × 106 CFUs per mL) cocultured in fibrin gels with 3 × 106 neutrophils per mL (n = 15), 5 × 106 neutrophils per mL (n = 22), and 10 × 106 neutrophils per mL (n = 17). (C) Propidium iodide was added to cocultures of SA-GFP (5 × 106 CFUs per mL) and 10 × 106 neutrophils per mL in fibrin gel to determine viability and containment capacity of neutrophils simultaneously. Lines represent median fluorescence intensity (n = 4). (D) Effect of addition of the phagocytosis inhibitor cytochalasin D (20 μM end concentration) on TUO during coculture of 10 × 106 polymorphonuclear (PMN) cells per mL and 5 × 106 CFUs per mL SA-GFP in fibrin gel at different (early) time points (n = 4). (E) Effect of neutrophil lysis by Triton X-100 (0.5% end concentration) on TUO during coculture of 10 × 106 PMN cells per mL and 5 × 106 CFUs per mL SA-GFP in fibrin gel at different (late) time points (n = 4). (F) Correlation between TUO and amount of bacteria present in fibrin gels. Various amounts of SA-GFP were added to fibrin gels, and TUO was determined (representative experiment; n = 5). (G) TUO after lysis of 10 × 106 PMN cells per mL at the start vs lysis at 16 hours when cocultured with 5 × 106 CFUs per mL SA-GFP in fibrin gel (n = 9). Data in panels A-B are mean + SEM. Data in panels E-G are mean ± SEM. *P < .05; **P < .01; ***P < .001 as determined by 1-way analysis of variance (ANOVA) or repeated measures (RM) 1-way ANOVA with the Greenhouse-Geisser correction followed by Tukey’s multiple comparison test or Student t test for paired samples. ns, not significant. Pieter H. C. Leliefeld et al. Blood Adv 2018;2:1344-1355 © 2018 by The American Society of Hematology