Endothelial cell harvest for seeding vascular prostheses: The influence of technique on cell function, viability, and number  Bernard M. Bourke, M.B.B.S.,

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Presentation transcript:

Endothelial cell harvest for seeding vascular prostheses: The influence of technique on cell function, viability, and number  Bernard M. Bourke, M.B.B.S., F.R.A.C.S., William R. Roche, M.B., M.Sc., Michael Appleberg, F.C.S.(S.A.), F.R.A.C.S.  Journal of Vascular Surgery  Volume 4, Issue 3, Pages 257-263 (September 1986) DOI: 10.1016/0741-5214(86)90195-3 Copyright © 1986 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 1 Scanning electron micrograph shows luminal surface of normal sheep vein (original magnification, × 2000). Journal of Vascular Surgery 1986 4, 257-263DOI: (10.1016/0741-5214(86)90195-3) Copyright © 1986 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 2 Scanning electron micrograph shows everted vein (after irrigation and spinning). Cellular lining has been completely removed. (Original magnification, × 275.) Journal of Vascular Surgery 1986 4, 257-263DOI: (10.1016/0741-5214(86)90195-3) Copyright © 1986 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 3 Scanning electron micrograph shows cannulated vein. Endothelial lining has been removed. Arrow indicates area where cells appear to be coming off in sheets. (Original magnification, × 275.) Journal of Vascular Surgery 1986 4, 257-263DOI: (10.1016/0741-5214(86)90195-3) Copyright © 1986 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 4 Endothelial cell counts from varying lengths of donor vein (two separate experiments). Counts on the ordinate are total counts per length under study, not counts per square centimeter. Journal of Vascular Surgery 1986 4, 257-263DOI: (10.1016/0741-5214(86)90195-3) Copyright © 1986 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions