Characterization results of the lysis device in the final system using 3OC12HSL. Characterization results of the lysis device in the final system using.

Slides:

Advertisements

Similar presentations

From: An Anti–VEGF-B Antibody Fragment Induces Regression of Pre-Existing Blood Vessels in the Rat Cornea Invest. Ophthalmol. Vis. Sci ;58(9):

Advertisements

Microtubule perturbations cause Ste5 patches to form less reliably, delay patch formation, and cause patches to persist for less time Microtubule perturbations.

Microtubule perturbations affect pathway variability η2(P) and transmitted signal P at or upstream of the Ste5 recruitment step Microtubule perturbations.

Growth rate‐dependent gene expression under glucose limitation.

Abundance of proteins matching selected subcellular locations and functions in CaCo‐2 cells. Abundance of proteins matching selected subcellular locations.

Simulation of oxygen and substrate demands for ammonia detoxification.

Schematic diagram of the three circuits analysed in this work.

Periodic changes in distribution of ERK–GFP fusion protein in cells after stimulation with EGF. (A) Confocal image of cells expressing ERK–GFP both before.

Melting behavior of proteins identified in the Escherichia coli meltome and their properties Melting behavior of proteins identified in the Escherichia.

Quantitative proteomics reveals daf‐16‐mediated reduction in protein metabolism in long‐lived daf‐2(e1370) mutants. Quantitative proteomics reveals daf‐16‐mediated.

Analysis of mutational effects of parameters on the phenotype (i. e

Analysis of in silico flux changes along the exponential growth phase: (A) In silico flux changes from 24 to 36 h, from 36 to 48 h, and from 48 to 60 h.

Characterization of the inferred factor associated with the differentiation state of the cell of origin Characterization of the inferred factor associated.

Proteins with hotspot mutations are often in the interaction networks with known cancer driver proteins Proteins with hotspot mutations are often in the.

The TBON model. The TBON model. (A) Representative confocal images of E14Tg2A cells stained for Tcf3 (green), Nanog (red), Oct4 (magenta), and total β‐catenin.

Dose response of pAGA1 and pFIG1 induction

Isolation of transcription elongation complexes from the 5′ and 3′ regions of a single gene Isolation of transcription elongation complexes from the 5′

The human STRIPAK complex associates with RASF3 and MST1/2

Correlating protein to mRNA and proteins per mRNA ratios

Characterization of promoters relative to pAGA1

Secretion assays are shown for pCASP plasmid, strain variations, and silks. Secretion assays are shown for pCASP plasmid, strain variations, and silks.

Widespread modulation of epigenetic landscape for differentiated organoids is driven by Hnf4g Widespread modulation of epigenetic landscape for differentiated.

Downstream antagonism.

DNA and membrane binding of MinD are interconnected.

Mutants of the Group III have differentially impaired induction of pAGA1 and pFIG1 Mutants of the Group III have differentially impaired induction of pAGA1.

Validation of some ePTL candidates.

The limiting, degrading entity hypothesis

Sensor optimization for thiosulfate and tetrathionate detection in the gut Sensor optimization for thiosulfate and tetrathionate detection in the gut A–F(A.

Lag time to division depends on the frequency of pulsed glucose for a subpopulation Lag time to division depends on the frequency of pulsed glucose for.

Changes to the growth conditions break the circuit by changing host gene expression Changes to the growth conditions break the circuit by changing host.

Titrations of other division proteins support FtsZ as the division limitation Titrations of other division proteins support FtsZ as the division limitation.

Fibroblast proliferation and ECM changes during wound healing

Effect of the loss of Kar4 on the induction of various promoters

Dynamics and expression level of mating‐dependent promoters

Chemical inhibitors of microtubule function attenuate signal but do not affect pathway variability Chemical inhibitors of microtubule function attenuate.

Computational wound healing gradients and in vivo imaging of the wound bed at PW2 (related to Fig 6)‏ Computational wound healing gradients and in vivo.

Francis Crick's central dogma of biology revolves around the transcription of mRNA from DNA, the translation of proteins from mRNA, and the degradation.

QRT–PCR analysis of tissue‐specific candidate gene expression in response to reduced IIS qRT–PCR analysis of tissue‐specific candidate gene expression.

Calibration of a population‐average model

Dynamics of induction of mating promoters after pheromone stimulation

Effects of ERK–GFP expression levels and cell density on ERK phosphorylation and oscillations. Effects of ERK–GFP expression levels and cell density on.

Impact of growth phase on the Escherichia coli meltome and proteome

Antibody analysis of Erk.

Subnetworks enriched for the hallmarks of cancer.

Biofilm inhibition assay with engineered E. coli.

Distinct collagen structures in the upper and lower neonatal dermis (related to Fig 1)‏ Distinct collagen structures in the upper and lower neonatal dermis.

Effect of HDAC inhibition on the steady‐state and dynamic transcriptional response and associated noise. Related to Fig 7 Effect of HDAC inhibition on.

A multitiered approach to characterize transcriptome structure.

Protein interactions at the nuclear pore.

Noise in hoxb1a/krox20 expression leads to boundary sharpening.

Melting behavior of protein complexes

In vivo imaging of the wound bed at later time points (related to Fig 7)‏ In vivo imaging of the wound bed at later time points (related to Fig 7) A–CRepresentative.

TIPI mediates rapid degradation of proteins in yeast.

Nedd4 proteins. Nedd4 proteins. (A) Schematic representation of rNedd4‐1, hNedd4‐1 and hNedd4‐2 (not to scale), with % amino‐acid identity between them.

Antisense expression associates with larger gene expression variability. Antisense expression associates with larger gene expression variability. (A–D)

Global and focused views of human interaction map.

BT_0370 galactokinase and BT_371 glucose/galactose transporter

Functional metabolic rearrangements in chloramphenicol‐resistant populations Functional metabolic rearrangements in chloramphenicol‐resistant populations.

SP3 provides an efficient means for preparing protein and peptide samples for MS analysis Schematic of the SP3 workflow in a single tube. SP3 provides.

Competence initiation during the progression to spore formation.

Functionality of smORFs Venn diagram showing the number of smORF proteins identified by MS in different experiments. Functionality of smORFs Venn diagram.

Perturbation of ligand depletion affects the ultrasensitivity of long‐term P‐Smad2 responses to different doses of TGF‐β stimulation. Perturbation of ligand.

(A) Observed significant protein fold‐changes during the fed–fasting transition in C57BL/6J (B6) and 129Sv (S9) mice fed with a normal diet (T0). (A) Observed.

Proteomic analysis of human transcription factors Disease correlation of 19 TFs and 4 well‐studied FOX family members, based on their GO annotations. Proteomic.

Inhibitory effect of perhexiline on the proliferation of the HepG2 cell line A, BPerhexiline was used to mimic the effect of the l‐carnitine analog on.

Fraction of flux entering the PEP‐glyoxylate cycle as a function of hexose uptake rate in batch (A) and chemostat (B) cultures. Fraction of flux entering.

Tangle formation and downregulation of miR‐132‐3p in LOAD A‐G

CRISPR‐Cas9 gene perturbation profiling in HeLa cells

The effects of laminarin, PMA and zymosan on PKC phosphorylation.

Mean CO2 release rates (averaged across all individuals tested) versus PO2 for each species of (A) tenebrionid and (B) scarabaeid. Mean CO2 release rates.

Presentation transcript:

Characterization results of the lysis device in the final system using 3OC12HSL. Characterization results of the lysis device in the final system using 3OC12HSL. (A) SDS–PAGE of (i, ii) total extracellular proteins and (iii–viii) IMAC purified His‐tagged S5 protein sampled from the extracellular supernatant. Total extracellular proteins exported from (i) E. coli carrying pTetR‐LasR‐pLuxR‐S5 (without lysis device) was significantly lesser than that exported from (ii) E. coli carrying pTetR‐LasR‐pLuxR‐S5‐pLuxR‐E7 (the final system) as indicated in darker lanes of (ii) relative to (i). (iii–v): E. coli carrying pTetR‐LasR‐pLuxR‐S5 (without lysis device) at 0, 2, and 4 h after induction. (vi–viii) E. coli carrying pTetR‐LasR‐pLuxR‐S5‐pLuxR‐E7 (the final system) at 0, 2, and 4 h after induction. The results show that pyocin S5 (57 kDa; arrowed) was only detectable in lanes that corresponded to E. coli carrying the final system and not in lanes of E. coli without the lysis device. Ladder used was Bio‐Rad's Precision Plus Protein standards. (B) Characterization of lysis device in the final system by optical density (bar graphs) and concentration of pyocin released (lines) after induction. The results show an impulse release of pyocin S5 at 2 h after induction, followed by a sustained steady‐state release in the final system (dotted lines). Optical density of the final system was characterized by an initial decrease at 2 h after induction, indicative of the onset of lysis, after which the regrowth of engineered E. coli occurs (shaded bar). Correspondingly, the concentration of pyocin released in E. coli without the lysis device (solid line) was 1/8 that of the final system with a continually increasing optical density (unshaded bar). Error bar represents the standard deviation of two replicates. Source data is available for this figure at www.nature.com/msb. Nazanin Saeidi et al. Mol Syst Biol 2011;7:521 © as stated in the article, figure or figure legend