PKAN human neurons show altered oxidative status PKAN human neurons show altered oxidative status Representative images of neurons stained with the neuron‐specific anti‐NCAM antibody, ROS‐sensitive fluorescent probe DCF, and the nuclear dye Hoechst. Scale bar 20 μm. Plots of the DCF fluorescence signal from NCAM‐positive control and PKAN human neurons, infected or not with Ngn2‐PANK2‐LV. Data presented as means + SEM of at least three independent experiments (one‐way ANOVA).Representative images of neurons stained with ThiolTracker Violet and the anti‐Tuj1. Scale bar 20 μm. ThiolTracker Violet fluorescence signal from Tuj1‐positive neurons was quantified and shown in the plots. Data presented as means + SEM of at least three independent experiments (unpaired, two‐tailed t‐test). Daniel I Orellana et al. EMBO Mol Med. 2016;8:1197-1211 © as stated in the article, figure or figure legend