Volume 68, Issue 2, Pages (August 2005)

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Volume 68, Issue 2, Pages 562-568 (August 2005) Increased levels of circulating endothelial cells in chronic periaortitis as a marker of active disease Gabriella Moroni, Nicoletta Del Papa, Lorenza Mazzeo Moronetti, Claudio Vitali, Wanda Maglione, Denise P. Comina, Francesca Urgnani, Sandro Sandri, Claudio Ponticelli, Agostino Cortelezzi Kidney International Volume 68, Issue 2, Pages 562-568 (August 2005) DOI: 10.1111/j.1523-1755.2005.00434.x Copyright © 2005 International Society of Nephrology Terms and Conditions

Figure 1 Flow cytometry evaluation of circulating endothelial cells (CECs). (A) Representative panel showing the analysis gate used to exclude platelets and debris (left), and the gate used to exclude CD45-positive hematopoietic cells (right). (B) Representative panels showing the negative control, total, and microvascular CECs in chronic periaortitis. PerCP, peridin chlorophyll protein; PE, phycoerythrin; FITC, fluorescein isothiocyanate. Kidney International 2005 68, 562-568DOI: (10.1111/j.1523-1755.2005.00434.x) Copyright © 2005 International Society of Nephrology Terms and Conditions

Figure 2 Levels of circulating endothelial cells (CECs) in peripheral blood obtained from patients with active chronic periaortitis (CP), healthy (HC), and atherosclerotic (ATS) controls. Data are shown as box plots, with upper and lower quartiles (shaded areas) and medians (horizontal lines within shaded areas). The number of CECs in CP patients was significantly higher when compared with those in controls. **P < 0.001. Kidney International 2005 68, 562-568DOI: (10.1111/j.1523-1755.2005.00434.x) Copyright © 2005 International Society of Nephrology Terms and Conditions

Figure 3 Cell numbers during the course of immunosuppressive therapy in patients with active disease. Kidney International 2005 68, 562-568DOI: (10.1111/j.1523-1755.2005.00434.x) Copyright © 2005 International Society of Nephrology Terms and Conditions

Figure 4 The activated phenotype of CECs was defined by P1H12 and CD62 surface expression. Data are shown as box plots, with upper and lower quartiles (shaded areas) and medians (horizontal lines within shaded areas). The number of activated CECs was markedly increased in patients with CP in comparison with atherosclerotic (ATS) and healthy (HC) controls. *P = 0.005. Kidney International 2005 68, 562-568DOI: (10.1111/j.1523-1755.2005.00434.x) Copyright © 2005 International Society of Nephrology Terms and Conditions