Autocrine effects of nitric oxide on HCO3- transport by rat thick ascending limb  Pablo A. Ortiz, Jeffrey L. Garvin  Kidney International  Volume 58, Issue 5, Pages 2069-2074 (November 2000) DOI: 10.1111/j.1523-1755.2000.00379.x Copyright © 2000 International Society of Nephrology Terms and Conditions

Figure 1 Effect of 0.5 mmol/L L-arginine (L-Arg) on rat thick ascending limb (TAL) bicarbonate flux (JHCO3-). Adding L-Arg to the basolateral bath decreased bicarbonate absorption from 35.4 ± 4.6 to 23.2 ± 2.9 pmol · mm-1· min-1. *P < 0.025, N = 7. Kidney International 2000 58, 2069-2074DOI: (10.1111/j.1523-1755.2000.00379.x) Copyright © 2000 International Society of Nephrology Terms and Conditions

Figure 2 Effect of D-Arg on rat TAL JHCO3-. Incubation of perfused TALs with 0.5 mmol/L D-Arg did not affect bicarbonate absorption compared with control (35.0 ± 4.0 vs. 37.4 ± 4.8 pmol · mm-1· min-1, N = 7). Kidney International 2000 58, 2069-2074DOI: (10.1111/j.1523-1755.2000.00379.x) Copyright © 2000 International Society of Nephrology Terms and Conditions

Figure 3 Effect of 0.5 mmol/L L-Arg on TAL JHCO3- during NO synthesis blockade. In tubules pretreated with 5 mmol/L L-NAME, the addition of 0.5 mmol/L L-Arg did not alter bicarbonate absorption (N = 7). Kidney International 2000 58, 2069-2074DOI: (10.1111/j.1523-1755.2000.00379.x) Copyright © 2000 International Society of Nephrology Terms and Conditions

Figure 4 Bicarbonate flux recovery. Removal of 0.5 mmol/L L-Arg from the basolateral bath generated a significant increment in TAL JHCO3-. After the tubules recovered from L-Arg treatment, bicarbonate absorption rose from 26.2 ± 3.9 to 34.8 ± 3.2 pmol · mm-1· min-1. *P < 0.01, N = 7. Kidney International 2000 58, 2069-2074DOI: (10.1111/j.1523-1755.2000.00379.x) Copyright © 2000 International Society of Nephrology Terms and Conditions

Figure 5 Effect of cGMP analogues on TAL JHCO3-. Adding the cGMP analogues 8-Br-cGMP 50 μmol/L (•) and dibutyryl-cGMP 50 μmol/L (○) to the bath significantly decreased bicarbonate absorption from 24.8 ± 2.1 to 16.9 ± 1.7 pmol · mm-1· min-1 (N = 10). *P < 0.05. Kidney International 2000 58, 2069-2074DOI: (10.1111/j.1523-1755.2000.00379.x) Copyright © 2000 International Society of Nephrology Terms and Conditions

Figure 6 Effect of 0.5 mmol/L L-Arg on TAL JHCO3- during protein kinase G blockade. In tubules pretreated with 2 μmol/L KT-5823, the addition of 0.5 mmol/L L-Arg did not alter bicarbonate absorption (N = 5). Kidney International 2000 58, 2069-2074DOI: (10.1111/j.1523-1755.2000.00379.x) Copyright © 2000 International Society of Nephrology Terms and Conditions