Adaptamers Target A Target B Adaptamer.

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Presentation transcript:

Adaptamers Target A Target B Adaptamer

Why develop adaptamers? To offer a new way to target substrates to cells (adaptamer linking cell surface and substrate). To create a tool for studying cell-cell interactions (adaptamer linking two cell surfaces). To provide a foundation for engineered catalysts (adaptamers linking 2 proteins).

Thrombin-binding domain Streptavidin-binding domain Thrombin Linking region Streptavidin

Adaptamer testing Bead surface streptavidin adaptamer thrombin anti-thrombin antibody Bead surface

Adaptamer functionality Adaptamer = A35 Adaptamer = A15 adaptamer Control: no thrombin streptavidin Control: beads lack streptavidin thrombin Bead surface

Adaptamer quenching… + + Results: Displacement strand Displacement strand added + No displacement strand added Background fluorescence