Fragmentation of DNA in morphologically normal human spermatozoa

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Fragmentation of DNA in morphologically normal human spermatozoa Conrado Avendaño, M.Sc., Anahí Franchi, Ph.D., Steven Taylor, Ph.D., Mahmood Morshedi, Ph.D., Silvina Bocca, M.D., Ph.D., Sergio Oehninger, M.D., Ph.D. Fertility and Sterility Volume 91, Issue 4, Pages 1077-1084 (April 2009) DOI: 10.1016/j.fertnstert.2008.01.015 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Incidence of spermatozoa with DNA fragmentation (TUNEL positive) in the fertile (FER), subfertile (SF) and infertile (INF) groups. A significant difference was found between FER and INF groups (∗P<.05). Fertility and Sterility 2009 91, 1077-1084DOI: (10.1016/j.fertnstert.2008.01.015) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Representative photomicrographs of the simultaneous assessment of normal sperm morphology and DNA fragmentation following swim up separation (left side: phase contrast; right side: TUNEL fluorescence). A: a normal spermatozoon (N) with DNA fragmentation; note three morphologically abnormal spermatozoa without DNA fragmentation; B: a normal spermatozoon (N) with DNA fragmentation; note several amorphous spermatozoa without DNA fragmentation; C: two normal spermatozoa (N), one of them with DNA fragmentation. Inset: photomicrograph at lower magnification showing the two normal spermatozoa in the same field. Fertility and Sterility 2009 91, 1077-1084DOI: (10.1016/j.fertnstert.2008.01.015) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Representative photomicrographs of the simultaneous assessment of normal sperm morphology and DNA fragmentation following swim up separation (left side: phase contrast; right side: TUNEL fluorescence). A: a normal spermatozoon (N) without DNA fragmentation; note several abnormal spermatozoa, one of them with DNA fragmentation; B: a normal (N) and an abnormal spermatozoa without DNA fragmentation; and C: an abnormal spermatozoon with DNA fragmentation. Fertility and Sterility 2009 91, 1077-1084DOI: (10.1016/j.fertnstert.2008.01.015) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions