Pathological Findings in TRUS Prostatic Biopsy—Diagnostic, Prognostic and Therapeutic Importance  Rodolfo Montironi, Roberta Mazzucchelli, Vincenzo Scattoni, David G Bostwick  European Urology Supplements  Volume 1, Issue 6, Pages 60-75 (September 2002) DOI: 10.1016/S1569-9056(02)00060-X

Fig. 1 (A) Prostate cancer: acini with an irregular, haphazard arrangement, randomly scattered in the stroma in clusters. (B) The cytologic features of adenocarcinoma include nuclear and nucleolar enlargement, which occurs in most malignant cells (nuclei from normal are shown for comparison). (C) Antikeratin 34βE12 stains nearly all of the normal basal cells of the normal prostate; no staining occurs in the secretory and stromal cells. (D) Prostate cancer cells do not react with antikeratin 34βE12, e.g. the basal cell layer is absent. (E) Needle biopsy with cancer within adipose; e.g. extraprostatic extension (EPE; see box). (F) Enlarged area of EPE. (G) Minute collections of small acini that raise the suspicion of carcinoma but fall below the diagnostic threshold (ASAP). (H) In a deeper section immunohistochemistry against 34βE12 shows absence of basal cell layer. (I) High-grade PIN. (J) High-grade PIN (with NE differentiation detected with immunohistochemistry against Chromogranin A; from the same case of (I) (arrow) and adjacent acini suspicious for malignancy; the additional arrows indicate the basal cells. (L) Prostate cancer (detected on repeat biopsy in the patient whose previous biopsy is shown in G). European Urology Supplements 2002 1, 60-75DOI: (10.1016/S1569-9056(02)00060-X)