Yajuan He, Patty K. Young, Frederick Grinnell

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Identification of Proteinase 3 as the Major Caseinolytic Activity in Acute Human Wound Fluid Yajuan He, Patty K. Young, Frederick Grinnell Journal of Investigative Dermatology Volume 110, Issue 1, Pages 67-71 (January 1998) DOI: 10.1046/j.1523-1747.1998.00075.x Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Burn fluid shows increased caseinolytic activity and intact fibronectin during the first few days after injury. Samples (60 mg) of blister fluid (B) and burn fluid collected on the days indicated were (a) subjected to casein zymography or (b) immunoblotted with an antibody against fibronectin. Purified human fibronectin (FN) (0.2 mg) was used as a standard for immunoblotting. Journal of Investigative Dermatology 1998 110, 67-71DOI: (10.1046/j.1523-1747.1998.00075.x) Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 The elastase inhibitor MDL blocks caseinolytic activity in burn fluid. Samples (75 mg) of burn fluid (day 3) were subjected to casein zymography. MDL (100 mM) or aprotinin (0.2mg per ml) were added to the reaction buffer where indicated. Journal of Investigative Dermatology 1998 110, 67-71DOI: (10.1046/j.1523-1747.1998.00075.x) Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Gel filtration chromatography shows that a single peak contains an elastase-related enzyme and most of the caseinolytic activity. Aliquots of the fractions from gel filtration HPLC were subjected to casein zymography (a) (15 ml) or incubated with the elastase substrate (b, c) (10 ml). Levels of caseinolytic activity were quantitated by image capture and computer analysis and are expressed as arbitrary units. Substrate degradation at the times indicated was determined by measuring OD410. Journal of Investigative Dermatology 1998 110, 67-71DOI: (10.1046/j.1523-1747.1998.00075.x) Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 The caseinolytic profile of purified PR-3 resembles the caseinolyic profile of burn fluid. Samples of burn fluid (BF, day 3) (75 mg) or purified PR-3 as indicated were subjected to casein zymography. In (b), MDL (100 mM) was added to the reaction buffer. Journal of Investigative Dermatology 1998 110, 67-71DOI: (10.1046/j.1523-1747.1998.00075.x) Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 The inhibitor SLPI selectively blocks the caseinolytic activity of human neutrophil elastase but not PR-3 or burn fluid. Samples of burn fluid (75 mg) (BF, day 3), the peak fraction (10 ml) from DEAE HPLC (F), purified PR-3 or human neutrophil elastase (HNE) as indicated were subjected to casein zymography. In (b), recombinant SLPI (4mg per ml) was added to the reaction buffer. Journal of Investigative Dermatology 1998 110, 67-71DOI: (10.1046/j.1523-1747.1998.00075.x) Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 SLPI inhibition of in vitro enzyme activity indicates that burn fluid contains a mixture of PR-3 and elastase. Samples of burn fluid (day 3) (100 mg) (a), PR-3 (200 ng) (b), or human neutrophil elastase (4 ng) (c) were incubated with the elastase substrate. Recombinant SLPI (0.8mg per ml) was added to the reaction buffer where indicated. Substrate degradation was determined at the time indicated by measuring OD410. Journal of Investigative Dermatology 1998 110, 67-71DOI: (10.1046/j.1523-1747.1998.00075.x) Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 7 Most caseinolytic activity can be cleared from burn fluid by immunoprecipitation with anti-PR-3 antibodies. Samples (500 mg) of burn fluid (BF, day 3) were pretreated with protein G agarose and then the supernatants were incubated with the monoclonal antibody against PR-3 (1:10, 0.2mg per ml) (ANCA) or control mouse IgG (1:10, 0.2mg per ml) (IgG) followed by protein G agarose. Aliquots of the pellets from the pretreatment (P1) and antibody/IgG (P2) incubations and the supernatants (S) after antibody/IgG treatment were subjected to casein zymography. Journal of Investigative Dermatology 1998 110, 67-71DOI: (10.1046/j.1523-1747.1998.00075.x) Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions