Xenotransplant cardiac chimera: immune tolerance of adult stem cells

Slides:

Advertisements

Similar presentations

The coronary delivery of marrow stromal cells for myocardial regeneration: Pathophysiologic and therapeutic implications Jih-Shiuan Wang, MD, Dominique.

Advertisements

Grafting an Acellular 3-Dimensional Collagen Scaffold Onto a Non-transmural Infarcted Myocardium Induces Neo-angiogenesis and Reduces Cardiac Remodeling

Paracrine Action Enhances the Effects of Autologous Mesenchymal Stem Cell Transplantation on Vascular Regeneration in Rat Model of Myocardial Infarction

Cell transplantation to prevent heart failure: a comparison of cell types Takeshiro Fujii, MD, Terrence M Yau, MD, MS, Richard D Weisel, MD, Nobuhisa.

Tracking cardiac engraftment and distribution of implanted bone marrow cells: Comparing intra-aortic, intravenous, and intramyocardial delivery Shu-Hong.

Bing-Yen Wang, MD, Hsiao-Huang Chang, MD, PhD, I

David D. Yuh, MD, Kimberly L. Gandy, MD, Bruce A

Mammalian Cardiac Regeneration After Fetal Myocardial Infarction Requires Cardiac Progenitor Cell Recruitment Myron Allukian, MD, Junwang Xu, PhD, Michael.

Tracking cardiac engraftment and distribution of implanted bone marrow cells: Comparing intra-aortic, intravenous, and intramyocardial delivery Shu-Hong.

Transplantation of hypoxia-preconditioned mesenchymal stem cells improves infarcted heart function via enhanced survival of implanted cells and angiogenesis

Therapeutic angiogenesis using autologous bone marrow stromal cells: improved blood flow in a chronic limb ischemia model Abdulaziz Al-Khaldi, MD, Hilal.

Cardiomyocyte Transplantation Improves Heart Function

Optimal time for cardiomyocyte transplantation to maximize myocardial function after left ventricular injury Ren-Ke Li, MD, PhD, Donald A.G. Mickle,

Cell transplantation to prevent heart failure: a comparison of cell types Takeshiro Fujii, MD, Terrence M Yau, MD, MS, Richard D Weisel, MD, Nobuhisa.

Comparative effects of mesenchymal progenitor cells, endothelial progenitor cells, or their combination on myocardial infarct regeneration and cardiac.

Hybrid Surgical Angiogenesis: Omentopexy Can Enhance Myocardial Angiogenesis Induced by Cell Therapy Taro Kanamori, MD, Go Watanabe, MD, Tamotsu Yasuda,

Targeting survival pathways to create infarct-spanning bridges of human embryonic stem cell–derived cardiomyocytes Jun Luo, MD, PhD, Matthew S. Weaver,

Paracrine Action Enhances the Effects of Autologous Mesenchymal Stem Cell Transplantation on Vascular Regeneration in Rat Model of Myocardial Infarction

Bing-Yen Wang, MD, Hsiao-Huang Chang, MD, PhD, I

Tracheal regeneration: Evidence of bone marrow mesenchymal stem cell involvement Agathe Seguin, MD, PhD, Sonia Baccari, MD, Muriel Holder-Espinasse, MD,

Total orthotopic heart transplantation for primary cardiac rhabdomyosarcoma: factors influencing long-term survival Daniel Grandmougin, MD, Georges Fayad,

Cell transplantation preserves cardiac function after infarction by infarct stabilization: Augmentation by stem cell factor Shafie Fazel, MD, MSc, Liwen.

Gene transfer to coronary artery bypass conduits

Myocardial tissue engineering with autologous myoblast implantation

Cardiac cell transplantation: closer to bedside

Endothelial sodding of the permaflow prosthetic coronary artery bypass conduit Michael R Phillips, MD, Hiroki Yamaguchi, MD, Virginia M Miller, PhD, Stuart.

Long-term patency of small-diameter vascular graft made from fibroin, a silk-based biodegradable material Soichiro Enomoto, MD, PhD, Makoto Sumi, MD,

Thomas E. Arnold, MD, Dmitri Gnatenko, PhD, Wadie F. Bahou, MD

Behavior of vital and killed autologous pericardium in the descending aorta of sheep David T. Cheung, PhD, Suk Jung Choo, MD, Albert C. Grobe, MS, Douglas.

Persistence of marrow stromal cells implanted into acutely infarcted myocardium: Observations in a xenotransplant model Derek J. MacDonald, MD, Jun Luo,

A novel vascularized patch enhances cell survival and modifies ventricular remodeling in a rat myocardial infarction model Qi Zhou, MD, PhD, Jian-Ye.

Abdominal Aorta Aneurysms in Children: Single-Center Experience of Six Patients Caisheng Ye, MD, PhD, Henghui Yin, MD, PhD, Ying Lin, MD, PhD, Li Zhou,

Autologous smooth muscle cell transplantation improved heart function in dilated cardiomyopathy Kyung-Jong Yoo, MD, Ren-Ke Li, MD, PhD, Richard D. Weisel,

Volume 23, Issue 2, Pages (June 2016)

Vascular endothelial growth factor transgene expression in cell-transplanted hearts Terrence M. Yau, MD, MSc, Guangming Li, MD, Richard D Weisel, MD,

Marrow Stromal Cells as Universal Donor Cells for Myocardial Regenerative Therapy: Their Unique Immune Tolerance Rony Atoui, MD, MS, Juan-Francisco Asenjo,

Therapeutic Potential of Human Umbilical Cord Derived Stem Cells in a Rat Myocardial Infarction Model Kai Hong Wu, MD, PhD, Bin Zhou, PhD, Cun Tao Yu,

Transbronchial gene transfer of endothelial nitric oxide synthase to transplanted lungs Anders Jeppsson, MD, Carlo Pellegrini, MD, Timothy O’Brien, MD,

Percutaneous Tissue-Engineered Pulmonary Valved Stent Implantation

Induced Pluripotent Stem Cell Transplantation in the Treatment of Porcine Chronic Myocardial Ischemia Yifu Zhou, MD, Suna Wang, MD, Zuxi Yu, MD, PhD,

The coronary delivery of marrow stromal cells for myocardial regeneration: Pathophysiologic and therapeutic implications Jih-Shiuan Wang, MD, Dominique.

Mark F Berry, MD, Y.Joseph Woo, MD The Annals of Thoracic Surgery

Spontaneous Hemothorax Caused by Pulmonary Micro-Venous Hemangioma

Improved heart function with myogenesis and angiogenesis after autologous porcine bone marrow stromal cell transplantation Shinji Tomita, MD, PhD, Donald.

Reconstruction of diaphragm using autologous fascia lata: an experimental study in dogs Kazuya Suzuki, PhD, Tsuyoshi Takahashi, MD, Yasushi Itou, MD,

Transplantation of cryopreserved cardiomyocytes

Nobuo Tsunooka, MD, PhD, Shin Hirayama, MD, PhD, Jeffrey A

Cellular therapy reverses myocardial dysfunction

Significant improvement of heart function by cotransplantation of human mesenchymal stem cells and fetal cardiomyocytes in postinfarcted pigs Jiang-Yong.

Layered implantation of myoblast sheets attenuates adverse cardiac remodeling of the infarcted heart Naosumi Sekiya, MD, Goro Matsumiya, MD, PhD, Shigeru.

Giant Cardiac Cavernous Hemangioma

Coronary artery and myocardial inflammatory reaction induced by intracoronary stent Walter J Gomes, MD, PhD, Osvaldo Giannotti-Filho, MD, PhD, Rodrigo.

Prevascularization with gelatin microspheres containing basic fibroblast growth factor enhances the benefits of cardiomyocyte transplantation Yutaka.

Leiomyomatous Hamartoma of the Posterior Mediastinum

Breast Infarction Due to Calciphylaxis After Coronary Artery Bypass Grafting Koen Cathenis, MD, Dominique Goossens, MD, Rudolf Vertriest, MD, Mieke Coppens,

Development of a spontaneously beating vein by cardiomyocyte transplantation in the wall of the inferior vena cava in a rat: A pilot study Wangde Dai,

Nitric oxide system in needle-induced transmyocardial revascularization Takayuki Saito, MD, PhD, Marc P Pelletier, MD, Hani Shennib, MD, Adel Giaid, PhD

Vascular Leiomyoma of the Pulmonary Artery

Angiogenesis and growth factor expression in a model of transmyocardial revascularization Marc P. Pelletier, MD, Adel Giaid, PhD, Sujith Sivaraman, BSc,

Histological confirmation of healing of gastrobronchial fistula using a muscle flap Manabu Okuyama, MD, Reijiro Saito, MD, Satoru Motoyama, MD, Michihiko.

Arteriovenous Malformation in the Anterior Mediastinum

Comparison of developmental endothelial locus-1 angiogenic factor with vascular endothelial growth factor in a porcine model of cardiac ischemia Murray.

Y. Castier, S. Lehoux, Y. Hu, G. Fonteinos, A. Tedgui, Q. Xu

Ren-Ke Li, MD, PhD, Richard D. Weisel, MD, Donald A. G

Selective cyclooxygenase 2 inhibitor induces indefinite survival of fully allogeneic cardiac grafts and generates CD4+ regulatory cells Takeshi Yokoyama,

Transplantation of cryopreserved muscle cells in dilated cardiomyopathy: Effects on left ventricular geometry and function Nobuhisa Ohno, MD, Paul W.M.

Optimizing cardiac cell therapy: From processing to delivery

Marrow stromal cells for cellular cardiomyoplasty: Feasibility and potential clinical advantages Jih-Shiuan Wang, MDa,b, Dominique Shum-Tim, MDa, Jacques.

Development of abnormal tissue architecture in transplanted neonatal rat myocytes Peter Whittaker, PhD, Jochen Müller-Ehmsen, MD, Joan S Dow, BS, Larry.

Beneficial effect of autologous cell transplantation on infarcted heart function: comparison between bone marrow stromal cells and heart cells Terrence.

Presentation transcript:

Xenotransplant cardiac chimera: immune tolerance of adult stem cells Takayuki Saito, MD, PhD, Jin-Qiang Kuang, MD, Bindu Bittira, MD, Abdulaziz Al-Khaldi, MD, Ray C.-J Chiu, MD, PhD The Annals of Thoracic Surgery Volume 74, Issue 1, Pages 19-24 (July 2002) DOI: 10.1016/S0003-4975(02)03591-9

Fig 1 Experimental design. (LCA = left coronary artery; MSCs = marrow stromal cells.) The Annals of Thoracic Surgery 2002 74, 19-24DOI: (10.1016/S0003-4975(02)03591-9)

Fig 2 Histochemical staining for β-galactosidase activity of mice marrow stromal cells in cultures. The transfected marrow stromal cells showed positive staining for β-galactosidase activity (blue color). (A) Culture-expanded mice marrow stromal cells before implantation. (B) Bone marrow specimen of the recipient rat to which mice marrow stromal cells were transplanted intravenously 13 weeks before. LacZ+ mice marrow stromal cells (arrow) were identified, whereas host marrow stromal cells were unstained. (C) Blood sample obtained at 1 day after ligating the left coronary artery in rats to which mice marrow stromal cells were transplanted intravenously 1 week before the ligation. LacZ+ mice marrow stromal cells (arrows) were also identified, whereas host cells were unstained. (Original magnification [A through C], ×100.) The Annals of Thoracic Surgery 2002 74, 19-24DOI: (10.1016/S0003-4975(02)03591-9)

Fig 3 Histochemical staining for β-galactosidase activity of mice marrow stromal cells in the gross heart. Gross heart specimen of the recipient rat was harvested at 12 weeks after coronary artery ligation. Mice marrow stromal cells were transplanted intravenously 1 week before the ligation. After fixation, the heart was stained for β-galactosidase activity of mice marrow stromal cells. Bluish discoloration can be seen on the infarcted myocardium, whereas noninfarcted myocardium remains unstained. (A) Frontal view. (B) Lateral view. The Annals of Thoracic Surgery 2002 74, 19-24DOI: (10.1016/S0003-4975(02)03591-9)

Fig 4 Histology and immunohistochemistry of the hearts with myocardial infarction. Mice marrow stromal cells were transplanted intravenously 1 week before coronary artery ligation. (A and B) Heart sample harvested at 8 weeks after ligation, and stained for β-galactosidase activity and hematoxylin and eosin. Numerous mice marrow stromal cells were identified mostly in the infarcted myocardium. (C) Heart sample harvested at 1 day after ligation. LacZ+ mice marrow stromal cells (arrows) were seen near vessel (asterisk) in an intact area. (D) Heart sample harvested at 1 day after ligation. LacZ+ cell (arrow) was seen in the interstitium of an intact myocardium. (E) Heart sample harvested at 12 weeks after ligation. LacZ+ cell (arrow) was morphologically integrated into the scar formation. (F) Heart sample harvested at 12 weeks after ligation, and immunostained for α-smooth muscle actin. LacZ+ cell (arrow) was seen in the vascular wall in the infarcted myocardium. (G) Heart sample harvested at 12 weeks after ligation, and immunostained for MF20. LacZ+ cells (arrows) had immunoreactivity for sarcomeric myosin heavy-chain molecules in their cytoplasms. (H) Heart sample harvested at 12 weeks after ligation, and immunostained for troponin I-C. LacZ+ cells (arrows) had immunoreactivity for troponin I-C in their cytoplasms. Original magnifications: (A) ×100; (B) ×200; (C through F) ×400; (G and H) ×1000. The Annals of Thoracic Surgery 2002 74, 19-24DOI: (10.1016/S0003-4975(02)03591-9)