S. aureus recovered from the airways of a cystic fibrosis patient.

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S. aureus recovered from the airways of a cystic fibrosis patient. S. aureus recovered from the airways of a cystic fibrosis patient. (A) Gram staining of a sputum specimen from a CF patient. Gram-positive cocci (black arrow) representative of S. aureus and numerous neutrophils (white arrow) are visible. (B) Mixture of large (black arrow) and small (white arrow) colonies cultured from a sputum sample, indicative of a mixture of normal S. aureus and S. aureus SCVs, on Columbia blood agar. (C) Growth of large (black arrow) and small (white arrow) colonies typical for a mixture of normal S. aureus and S. aureus SCVs on SAID chromogenic agar, used as a selective agar for S. aureus, from a sputum sample from a CF patient. (D) Pulsed-field gel electrophoresis of normal S. aureus and S. aureus SCVs, which persisted in the airways of an individual CF patient from 1994 until 2000. n, normal S. aureus; S, SCV; lane 1, marker; lane 2, normal S. aureus (NS) 6/1994; lanes 3 and 4, SCV and NS isolated together in September 1995; lanes 5 and 6, SCV and NS isolated together in June 1995; lanes 7 and 8, SCV and NS isolated in January 1997; lane 9, SCV from May 1998; lane 10, SCV from October 1999; lane 11, SCV from January 2000; lane 12, marker. Barbara C. Kahl et al. Clin. Microbiol. Rev. 2016; doi:10.1128/CMR.00069-15