Elevated non-esterified fatty acid concentrations during in vitro murine follicle growth alter follicular physiology and reduce oocyte developmental competence

Slides:

Advertisements

Similar presentations

Direct vitamin D3 actions on rhesus macaque follicles in three-dimensional culture: assessment of follicle survival, growth, steroid, and antimüllerian.

Advertisements

Rong Hu, Ph. D. , Fei-miao Wang, M. D. , Liang Yu, Ph. D. , Yan Luo, M

A giant oocyte in a cohort of retrieved oocytes: does it have any effect on the in vitro fertilization cycle outcome? Ronit Machtinger, M.D., Joseph.

Developmental programming: prenatal testosterone excess disrupts anti-Müllerian hormone expression in preantral and antral follicles Almudena Veiga-Lopez,

Gene expression analysis of a new source of human oocytes and embryos for research and human embryonic stem cell derivation Sharon F. Sneddon, Ph.D.,

Leukemia inhibitory factor induces cumulus expansion in immature human and mouse oocytes and improves mouse two-cell rate and delivery rates when it is.

Lapatinib inhibits meiotic maturation of porcine oocyte-cumulus complexes cultured in vitro in gonadotropin-supplemented medium Eva Nagyova, D.V.M.,

Acquisition and loss of oocyte meiotic and developmental competence during in vitro antral follicle growth in mouse Ingrid Segers, M.Sc., Tom Adriaenssens,

Cigarette smoke condensate exposure delays follicular development and function in a stage-dependent manner Jean Clair Sadeu, M.D., Ph.D., Warren G. Foster,

Developmental capacity of in vitro–matured human oocytes retrieved from polycystic ovary syndrome ovaries containing no follicles larger than 6 mm Luis.

Resistin decreases insulin-like growth factor I–induced steroid production and insulin- like growth factor I receptor signaling in human granulosa cells

MRNA expression pattern of insulin-like growth factor components of granulosa cells and cumulus cells in women with and without polycystic ovary syndrome.

Concentrations of stromal cell-derived factor-1 and vascular endothelial growth factor in relation to the diameter of human follicles Akemi Nishigaki,

Michael M. Alper, M.D. Fertility and Sterility

Composition of single-step media used for human embryo culture

Successful delivery after the transfer of embryos obtained from a cohort of incompletely in vivo matured oocytes at retrieval time Patricia Fauque, M.D.,

Impact of final oocyte maturation using gonadotropin-releasing hormone agonist triggering and different luteal support protocols on endometrial gene expression

Contrasting patterns in in vitro fertilization pregnancy rates among fresh autologous, fresh oocyte donor, and cryopreserved cycles with the use of day.

Guoying Dong, B. S. , Yueshuai Guo, B. S. , Huarong Cao, B. S

The antifibrotic drug halofuginone inhibits proliferation and collagen production by human leiomyoma and myometrial smooth muscle cells Meagan M. Grudzien,

The relative contribution of assisted reproductive technologies and ovulation induction to multiple births in the United States 5 years after the Society.

Expression of growth differentiation factor-9 and bone morphogenetic protein-15 in oocytes and cumulus granulosa cells of patients with polycystic ovary.

Improvement of in vitro culture of mouse cumulus–oocyte complexes using PDE3- inhibitor followed by meiosis induction with epiregulin Sergio Romero, M.Sc.,

Sara D. M. Valckx, Jessie De Bie, Ellen D. Michiels, Ilse G

Shi Ying Jin, Ph. D. , Lei Lei, Ph. D. , Ariella Shikanov, Ph. D

Xenotransplantation by injection of a suspension of isolated preantral ovarian follicles and stroma cells under the kidney capsule of nude mice Jan M.J.

Cell plating density alters the ratio of estrogenic to progestagenic enzyme gene expression in cultured granulosa cells Valério M. Portela, D.V.M., Ph.D.,

Age-associated alteration of oocyte-specific gene expression in polar bodies: potential markers of oocyte competence Ze-Xu Jiao, M.D., Ph.D., Min Xu,

Oocyte cryopreservation

Growth hormone, insulin-like growth factor I, and leptin interaction in human cultured lutein granulosa cells steroidogenesis Maria Karamouti, M.D, Panagoula.

Angela R. Baerwald, Ph. D. , Olufemi A. Olatunbosun, M. D. , Roger A

Time-lapse deselection model for human day 3 in vitro fertilization embryos: the combination of qualitative and quantitative measures of embryo growth

Reply of the Authors Fertility and Sterility

Effect of long-term exposure at suprazero temperatures on activity and viability of human ovarian cortex Evgenia Isachenko, Ph.D., Vladimir Isachenko,

Nuclear maturation and structural components of nonhuman primate cumulus–oocyte complexes during in vivo and in vitro maturation Jenna K. Nyholt de Prada,

Rahi Victory, M. D. , F. R. C. S. C. , Ghassan M. Saed, Ph. D

Effects of follicular phase and oocyte–cumulus complexes quality on the protein profile and in vitro oocyte meiosis competence in Cebus apella Sheyla.

Merja Bläuer, Ph. D. , Päivi H. Rovio, M. D. , Timo Ylikomi, M. D

Gene injections of vascular endothelial growth factor and growth differentiation factor-9 stimulate ovarian follicular development in immature female.

Immunohistochemical localization of growth factors after cryopreservation and 3 weeks' xenotransplantation of human ovarian tissue Anu David, M.Sc.,

Timed analysis of the nuclear maturation of oocytes in early preantral mouse follicle culture supplemented with recombinant gonadotropin Rita G Cortvrindt,

Impact of oocyte donation on perinatal outcome in twin pregnancies

Insulin-like growth factor-II (IGF-II), IGF-binding protein-3 (IGFBP-3), and IGFBP-4 in follicular fluid are associated with oocyte maturation and embryo.

Introduction Fertility and Sterility

Granulosa–lutein cell growth differentiation factor-9 (GDF-9) messenger RNA and protein expression in in vitro fertilization (IVF) cycles: relation to.

Estradiol modulation of hepatocyte growth factor by stromal fibroblasts in the female reproductive tract Kimberly D. Coleman, M.S., Jacqueline A. Wright,

Mitochondrial gene expression in granulosa cells of severe endometriosis with in vitro fertilization and embryo transfer Yoshiki Yamashita, M.D., Masako.

Veronica Bertini, Ph. D. , Angelo Valetto, Ph. D. , Angela Uccelli, Ph

Prospective cohort study of three- versus two-dimensional ultrasound for prediction of oocyte maturity Doron Shmorgun, M.D., Edward Hughes, M.D., Patrick.

Ri-Cheng Chian, Ph.D., Peter S. Uzelac, M.D., Geeta Nargund, M.D.

Derivation of developmentally competent oocytes by in vitro culture of preantral follicles retrieved from aged mice Jung Kyu Choi, B.Sc., Jong Il Ahn,

Cameron Gilbert, M. Sc. , Maria Valois, M. D. , FRCPC, Ph. D

Comparison of urinary and recombinant follicle-stimulating hormone in in vitro growth, maturation, and fertilization of mouse preantral follicles Giannina.

Effects of growth hormone and insulin-like growth factor 1 on progesterone production in human luteinized granulosa cells Toshiaki Taketani, M.D., Yoshiaki.

Effects of hyperglycemia on the differential expression of insulin and insulin-like growth factor-I receptors in human normal peritoneal and adhesion.

Human granulosa-lutein cell in vitro production of progesterone, inhibin A, inhibin B, and activin A are dependent on follicular size and not the presence.

L. Scott, J. Bernsten, K. DeLegge, J. Hill, N. Ramsing

Effect of vitrification and beta-mercaptoethanol on reactive oxygen species activity and in vitro development of oocytes vitrified before or after in.

Upregulation of mRNA expression of vascular endothelial growth factor and its receptors by exogenous human chorionic gonadotropin in cultured oviduct.

Production of macrophage inflammatory protein-3α in human follicular fluid and cultured granulosa cells Yasushi Kawano, M.D., Junichiro Fukuda, M.D.,

David H. Barad, M.D., M.S., Norbert Gleicher, M.D.

Prolactin inhibits oocyte release after gonadotropin stimulation in the rat: Putative mechanism involving ovarian production of beta-endorphin and prostaglandin

Transforming growth factor-β1 and its receptor soluble endoglin are altered in polycystic ovary syndrome during controlled ovarian stimulation Reshef.

Altered circulating levels of matrix metalloproteinases 2 and 9 and their inhibitors and effect of progesterone supplementation in women with endometriosis.

Ovarian follicular volume and follicular surface area are better indicators of follicular growth and maturation, respectively, than is follicular diameter

Ongoing pregnancy rates in intrauterine insemination are affected by late follicular- phase progesterone levels Antonio Requena, M.D., María Cruz, Ph.D.,

In vitro viability and secretory capacity of human luteinized granulosa cells after gonadotropin-releasing hormone agonist trigger of oocyte maturation

Effect of oocyte quality on blastocyst development after in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in a sheep model Maria.

Exposure to lipid-rich follicular fluid is associated with endoplasmic reticulum stress and impaired oocyte maturation in cumulus-oocyte complexes Xing.

Oocyte retrieval timing based on spontaneous luteinizing hormone surge during natural cycle in vitro fertilization treatment Daniel Bodri, M.D., M.Sc.,

Presentation transcript:

Elevated non-esterified fatty acid concentrations during in vitro murine follicle growth alter follicular physiology and reduce oocyte developmental competence Sara D.M. Valckx, M.Sc., Veerle Van Hoeck, Ph.D., Maria Arias-Alvarez, Ph.D., Veronica Maillo, M.Sc., Angela P. Lopez-Cardona, M.Sc., Alfonso Gutierrez-Adan, Ph.D., Mario Berth, Ph.D., Rita Cortvrindt, M.Sc., Peter E.J. Bols, Ph.D., Jo L.M.R. Leroy, Ph.D. Fertility and Sterility Volume 102, Issue 6, Pages 1769-1776.e1 (December 2014) DOI: 10.1016/j.fertnstert.2014.08.018 Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Follicle steroidogenesis. Estradiol and P concentrations in the culture medium of day-12 and day-13 follicles cultured in basal NEFA, high SA, high OA, or high NEFA conditions. Absolute E2 reduction and P rise between day 12 and day 13 are also shown. Data are presented as mean ± SD. a,b Different superscripts on a bar indicate a statistically significant difference between treatments (P<.05). Fertility and Sterility 2014 102, 1769-1776.e1DOI: (10.1016/j.fertnstert.2014.08.018) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Granulosa cell gene expression patterns. Comparison of relative transcript abundance in murine day-13 granulosa cells. Follicles were cultured for 12 days in basal NEFA, high SA, high OA, or high NEFA conditions (4 replicates), and granulosa cells were obtained on day 13, at 20 hours after a final maturation stimulus. a,b Different superscripts on a bar indicate a statistically significant difference between treatments (P<.05). Data are presented as relative transcript abundance ± SEM. Fertility and Sterility 2014 102, 1769-1776.e1DOI: (10.1016/j.fertnstert.2014.08.018) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions