Comparison of cartilage histopathology assessment systems on human knee joints at all stages of osteoarthritis development C. Pauli, R. Whiteside, F.L.

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Presentation transcript:

Comparison of cartilage histopathology assessment systems on human knee joints at all stages of osteoarthritis development C. Pauli, R. Whiteside, F.L. Heras, D. Nesic, J. Koziol, S.P. Grogan, J. Matyas, K.P.H. Pritzker, D.D. D’Lima, M.K. Lotz Osteoarthritis and Cartilage Volume 20, Issue 6, Pages 476-485 (June 2012) DOI: 10.1016/j.joca.2011.12.018 Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Histological assessment of the surface structure parameter according to MANKIN on sections from femoral condyles: (A) Normal (intact smooth surface), score 0. (B) Surface irregularities (undulations), score 1. (C) Pannus and surface irregularities (fibrillation), score 2. (D) Clefts to transitional zone, score 3. (E) Clefts to radial zone, score 4. (F) Clefts to calcified zone, score 5. (G) Complete disorganization, score 6. Safranin O - Fast Green, pictures taken with 4× and 40× objectives. Osteoarthritis and Cartilage 2012 20, 476-485DOI: (10.1016/j.joca.2011.12.018) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Histological assessment of cellularity according to MANKIN on sections from femoral condyles: (A) Normal (1-2 cells/chondron), score 0. (B) Diffuse hypercellularity, score 1. (C) Chondrocyte cloning (clusters), score 2. (D) Hypocellularity, score 3. Safranin O – fast green, pictures taken with 4× and 40× objectives. Osteoarthritis and Cartilage 2012 20, 476-485DOI: (10.1016/j.joca.2011.12.018) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Histological assessment of the Safranin O staining intensity parameter according to MANKIN on sections from femoral condyles. (A) Normal (staining except for surface zone), score 0. (B) Slight reduction (particularly superficial zone, score 1). (C) Moderate reduction (extending down to mid zone), score 2. (D) Severe reduction (entire cartilage thickness), score 3. (E) No dye noted, score 4. Safranin O-fast green stain, pictures were taken with 4× and 40× objectives. Osteoarthritis and Cartilage 2012 20, 476-485DOI: (10.1016/j.joca.2011.12.018) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Histological tidemark assessment according to MANKIN on sections from femoral condyles: (A) Tidemark intact, score 0. H&E stain, pictures taken with 4× and 40× objectives. (B) Tidemark crossed by blood vessels (±tidemark duplication), score 1. Safranin O - fast green stain, pictures taken with 4× and 40× objectives. Osteoarthritis and Cartilage 2012 20, 476-485DOI: (10.1016/j.joca.2011.12.018) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Summary plot of average MANKIN vs average OARSI system: Summary plot comprising 95% confidence ellipse, and marginal histograms of the frequency distribution. The graph shows a strong monotonic relationship between the two scoring systems, reflective of the Spearman rho value of 0.96, The relationship is roughly, but not perfectly, linear: Given a particular MANKIN [or OARSI] score, there is a fair amount of scatter around the corresponding OARSI [or MANKIN] scores. The marginal MANKIN and OARSI system distributions are rather flat. Osteoarthritis and Cartilage 2012 20, 476-485DOI: (10.1016/j.joca.2011.12.018) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Supplementary Fig. S1 Harvesting protocol: (A) For the femoral condyles medial and lateral sagittal osteochondral slabs were harvested as indicated by the red lines. (B) One coronal central slab of each tibia plateau was harvested as indicated. (C) From each patella a central transverse slab was harvested as indicated by the red lines. For histology processing each osteochondral slab was cut into tissue blocks at defined regions for histology, femoral condyle (D) and tibia (E). Osteoarthritis and Cartilage 2012 20, 476-485DOI: (10.1016/j.joca.2011.12.018) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions