Fig. 1 Neutrophils derived from patients with MPNs are associated with an increase in NET formation and a prothrombotic, NET-rich phenotype. Neutrophils.

Slides:

Advertisements

Similar presentations

Fig. 1. TP is highly expressed in myeloma.

Advertisements

Fig. 4. Primary human metastatic melanomas contain CCL21-expressing LECs, and expression of VEGFC positively correlates with hallmarks of tumor inflammation.

Osama M. El-Sayed, BS, Nicholas A. Dewyer, MD, Catherine E

Fig. 6. AZD6738 induces DNA damage and apoptosis and exhibits antitumor efficacy in xenograft models of high-risk medulloblastoma and neuroblastoma. AZD6738.

Fig. 1. FMRP deficiency leads to increased NSC activation in the adult dentate gyrus. FMRP deficiency leads to increased NSC activation in the adult dentate.

Fig. 3. Features of PH in KRasLA2 transgenic mice.

In vivo function of MeTro sealants using rat incision model of lungs

Fig. 2. Pharmacologic inhibition of ALK impairs STING activation.

Fig. 7. Role of PDE5 up-regulation in lung cancer–associated PH.

Fig. 5. Correlation of tail and long bone growth velocities with Cxm serum concentrations in mice. Correlation of tail and long bone growth velocities.

Neutrophil aggregation is selectively induced by PS+ platelets

Fig. 3. Obese IFN-γ−/− mice develop accelerated NAFLD with fibrosis.

Fig. 4. Functional annotation of VUS in EGFR.

Fig. 6. N95BA5 biocompatibility beyond intended use frame.

Fig. 4. Prion infectivity of skin samples from sCJD patients.

Fig. 8. mRIPO elicits neutrophil influx followed by DC and T cell infiltration into tumors. mRIPO elicits neutrophil influx followed by DC and T cell infiltration.

Fig. 4 Topical application of SAAP-148 ointment eradicates acute and established infections of MRSA and A. baumannii from the skin. Topical application.

Fig. 4. Characterization of human liver seed graft morphology.

Fig. 2. GPC3 expression in normal and tumor tissues.

Fig. 1. Paclitaxel delays tumor growth and promotes infiltration of TIE2hi/VEGFhi macrophages and TMEM assembly. Paclitaxel delays tumor growth and promotes.

Fig. 6. Effects of CD31-NP targeting in perfused human kidneys.

Fig. 4 Expression of cleaved caspase 3, PD-L1, and PD-1 in HGGs after reovirus treatment. Expression of cleaved caspase 3, PD-L1, and PD-1 in HGGs after.

Fig. 4. Specific versus nonspecific NP accumulation.

Fig. 5. Vascularization of human liver seed grafts.

Fig. 1. CD31 is present throughout the human renal vasculature.

Fig. 1. Aberrant JNK pathway activation in mouse models of ALS and in spinal cord tissue from patients with sporadic ALS. Aberrant JNK pathway activation.

Immunofluorescence staining of virus-infected human placental explants

Fig. 1. Generation of the ΔEx50 mouse model.

Fig. 2 In vitro assessment of hESC-RPE cell sheets.

Fig. 2 STED microscopy of isolated cardiomyocytes from mice treated with MP-rhodamine–loaded CaPs. STED microscopy of isolated cardiomyocytes from mice.

by Ling-juan Zhang, Christian F. Guerrero-Juarez, Tissa Hata, Sagar P

Representative CT and PET/CT images of three patients with NSCLCs

Fig. 7 BRD0705 impairs colony formation in AML cell lines and patient cells and shows in vivo efficacy in multiple AML mouse models. BRD0705 impairs colony.

Fig. 3. Genetically engineered PD-L1

Fig. 5 EGFR mutation status of patients with NSCLC, detected by histological examination and ARMS PCR. EGFR mutation status of patients with NSCLC, detected.

Fig. 7. NPs accumulate at sites of vascular obstruction.

Elysse C. Filipe et al. BTS 2018;3:38-53

Fig. 1. Drug combination screen identifies BETi as acting synergistically with PARPi. Drug combination screen identifies BETi as acting synergistically.

Fig. 5 Local gel scaffold for T cell memory response.

Fig. 4. Irisin protected against oxidative stress and apoptosis in IR-injured lung tissue. Irisin protected against oxidative stress and apoptosis in IR-injured.

Fig. 4. Improved tumor response to docetaxel in TNBC and trastuzumab in HER2-amplified PDX models with the addition of S Improved tumor response.

Elysse C. Filipe et al. BTS 2018;3:38-53

Figure Immunologic characterization

Fig. 7 CSPG4-high GBMs show more microglia than CSPG4-low GBMs and express TNFα. CSPG4-high GBMs show more microglia than CSPG4-low GBMs and express TNFα.

Fig. 4 PD-L1 expression is found in the spleen and the BM of mice transplanted with JAK2V617F-transduced bone marrow. PD-L1 expression is found in the.

PAAND is driven by local inflammasome activation and IL-1β production

Fig. 3. TKI sensitivity assessed by the MANO method.

Fig. 4. Features of PH in LLC1 lung tumor mice.

Fig. 4. The effect of single-dose rozanolixizumab on the concentration of IgG subtypes in healthy subjects. The effect of single-dose rozanolixizumab on.

Fig. 1. In vivo fates of patient-derived AML cells defined by mutational profile. In vivo fates of patient-derived AML cells defined by mutational profile.

Fig. 1 CSPG4 is expressed in GBM specimens and GBM-NS and associated with more aggressive disease. CSPG4 is expressed in GBM specimens and GBM-NS and associated.

Fig. 4. Acute lung injury in miR-223−/y mice.

Fig. 3 CSF1 is expressed in human melanoma.

Fig. 7 Analysis of the bacterial nidus within tissue abscesses by MALDI IMS demonstrates a paucity of calprotectin signal. Analysis of the bacterial nidus.

Fig. 6 Photoreceptor cell survival in the RCS rat retina after transplantation with hESC-RPE cell sheets. Photoreceptor cell survival in the RCS rat retina.

B cells infiltrate mouse and human pancreatic neoplasia and promote growth of KrasG12D-PDEC in vivo. B cells infiltrate mouse and human pancreatic neoplasia.

Correlation of reovirus RNA/protein with proliferating tumor cells

Fig. 6 Combination therapy with LVSOD2 and LVshCTGF preserves flap volume and reduces fibrosis after RT. Combination therapy with LVSOD2 and LVshCTGF preserves.

Fig. 2. BET inhibition enhances PARPi-induced DNA damage.

Fig. 4 Lentiviral transgene expression penetrates tissue and provides durable effects in vivo. Lentiviral transgene expression penetrates tissue and provides.

Fig. 3. Features of PH in KRasLA2 transgenic mice.

Genetic EGFR ablation in K-RAS–mutated lung AC reduces tumor growth

PTZ-induced c-Fos and Arc proteins in hippocampal dentate gyrus

Fig. 1. DEL-1 is expressed by human and mouse osteoclasts.

Fig. 1. Matrix stiffness sensitizes myofibroblasts to apoptosis induced by inhibition of their mechanotransduction pathways. Matrix stiffness sensitizes.

Fig. 4 IP6K1-mediated polyP production by platelets plays a critical role in LPS-induced NPA formation. IP6K1-mediated polyP production by platelets plays.

Fig. 6 Antitumor effect on tumor growth and pulmonary metastasis of CSSD-9 in vivo. Antitumor effect on tumor growth and pulmonary metastasis of CSSD-9.

Expression of Rapgef2 and Rapgef6 in the E13.5 and E15.5 brains.

Detection of microcalcifications in 4T1 mammary tumors and human breast tumor tissue. Detection of microcalcifications in 4T1 mammary tumors and human.

In vivo function of MeTro sealants using rat incision model of lungs

Presentation transcript:

Fig. 1 Neutrophils derived from patients with MPNs are associated with an increase in NET formation and a prothrombotic, NET-rich phenotype. Neutrophils derived from patients with MPNs are associated with an increase in NET formation and a prothrombotic, NET-rich phenotype. (A) NET formation in patients with myeloproliferative neoplasms (MPNs) (receiving a JAK inhibitor, n = 5; receiving other therapy, n = 14) compared to healthy controls (n = 11) when stimulated with 4 μM ionomycin (IO) or dimethyl sulfoxide (DMSO) for 2 hours. Patients receiving a JAK inhibitor are indicated by JAKI. Data are shown as individual values and medians. (B) Representative IF images of human neutrophils after stimulation with 4 μM IO or DMSO for 2 hours. 4′,6-Diamidino-2-phenylindole (DAPI) is shown in blue, and citrullinated histone H3 (H3cit) is shown in green. Scale bar, 50 μm. (C) Percentages of human neutrophils with evidence of NET formation after stimulation with phorbol 12-myristate 13-acetate (PMA) (10 nM) with and without ruxolitinib pretreatment (n = 4). Neutrophils are derived from controls. (D) Representative images of human neutrophils from healthy controls stimulated with PMA (10 nM) after 150 min of ex vivo pretreatment with DMSO, ruxolitinib (300 nM), or GSK484 (PAD4 inhibitor,10 μM). Scale bar, 50 μm. (E) Lung tissue sections from mice expressing the Jak2V617F mutation as compared to Jak2WT mice. Scale bar, 200 μm. (F) Characterization of clot content in the lungs of Jak2V617 mice. Hematoxylin and eosin (H&E) stain. Scale bar, 50 μm. VWF, von Willebrand factor. (G) Lung tissue sections from mice expressing the Jak2V617F mutation as compared to Jak2WT mice. Neutrophil infiltration and NETs are shown by neutrophil-specific Ly6G (red) and H3cit (green), respectively. Scale bar, 100 μm. (H) Percentages of mouse neutrophils with evidence of NET formation by morphological criteria (left) (n = 9 for all genotype/treatment combinations) or H3cit-positive staining (right) (n = 6 for all genotype/treatment combinations) grouped by genotype after stimulation with 4 μM IO or DMSO for 2 hours. (I) Representative IF images of mouse neutrophils derived from Jak2WT and Jak2V617F mice after stimulation with 4 μM IO or DMSO for 2 hours. DAPI is shown in blue, and H3cit is shown in green. Scale bar, 50 μm. Ofir Wolach et al., Sci Transl Med 2018;10:eaan8292 Published by AAAS