Pathological evaluation of the rat endometriosis model Ichiro Uchiide, M.D., Ph.D., Tomomi Ihara, M.D., Ph.D., Masao Sugamata, M.D., Ph.D.  Fertility and Sterility  Volume 78, Issue 4, Pages 782-786 (October 2002) DOI: 10.1016/S0015-0282(02)03327-7

FIGURE 1 Histopathologic features of autotransplantation-performed peritoneum. (A), Four days after uterine autotransplantation. (B), Seven days after uterine autotransplantation. (C), Fourteen days after uterine autotransplantation. (D), Seven days after mesentery autotransplantation. (All views: am = Abdominal muscle; magnification: ×40.) The proliferative lesions in the peritoneum adjacent to the implanted uterine tissue were more pronounced at 7 days after uterine autotransplantation (B) than at 4 days after autotransplantation (A); subsequently, the proliferation declined at 14 days after autotransplantation (C). None the peritoneum implanted with mesentery showed any appreciable change (D). Uchiide. Pathological evaluation of rat endometriosis model. Fertil Steril 2002. Fertility and Sterility 2002 78, 782-786DOI: (10.1016/S0015-0282(02)03327-7)

FIGURE 2 Localization of mast cells and plasma cells in the peritoneal stroma adjacent to implanted uterine tissue at 7 days after autotransplantation. (A), Toluidine blue stain. Arrow indicates mast cells (violet color; magnification: ×200). (B), Methylgreen pyronin stain. Arrow indicates plasma cells (red color; magnification: ×200). Infiltrations of numerous mast cells and plasma cells are observed diffusely. Uchiide. Pathological evaluation of rat endometriosis model. Fertil Steril 2002. Fertility and Sterility 2002 78, 782-786DOI: (10.1016/S0015-0282(02)03327-7)

FIGURE 3 Ultrastructural images of infiltrated cells in the peritoneal stroma adjacent to implanted uterine tissue after autotransplantation. (A), Four days after autotransplantation (M = mast cell). (B), Four days after autotransplantation. (E = apoptotic eosinophil). Fragmentation of nucleus with condensed chromatin is observed (∗). (C), Four days after autotransplantation (P = plasma cells). (D), Seven days after autotransplantation (M = mast cell; m = macrophage). The macrophage contains some phagosomes (∗). (E), Seven days after autotransplantation (E = apoptotic eosinophils; L = lymphocytes). Fragmentation eosinophil’s nucleus with condensed chromatin is observed (∗). (F), At 14 days after implantation. Eosinophils (E) and some macrophages (m) are observed. Some of the eosinophils show nuclear fragmentation (∗). (A–E: scale bar = 3 μm; F: scale bar = 4 μm.) Four days after autotransplantation, mast cells, eosinophils, and plasma cells are observed; some apoptotic eosinophils also were observed (A through C). Lymphocytes and macrophages, which phagocytose some apoptotic bodies, are observed at 7 days after autotransplantation (D and E). The stromal peritoneal tissue at 14 days after autotransplantation shows only apoptotic eosinophils and macrophages (F). Uchiide. Pathological evaluation of rat endometriosis model. Fertil Steril 2002. Fertility and Sterility 2002 78, 782-786DOI: (10.1016/S0015-0282(02)03327-7)