Purification and concentration of PCR products leads to increased signal intensities with fewer allelic drop-outs and artifacts L.M.I. Pedersen, M. Stangegaard,

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Presentation transcript:

Purification and concentration of PCR products leads to increased signal intensities with fewer allelic drop-outs and artifacts L.M.I. Pedersen, M. Stangegaard, H.S. Mogensen, N. Morling Forensic Science International: Genetics Supplement Series Volume 3, Issue 1, Pages e347-e348 (December 2011) DOI: 10.1016/j.fsigss.2011.09.036 Copyright © 2011 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Non-purified (A and C) and purified (B and D) amplificates from a trace evidence sample. A and B: Primer peaks. C and D: Electropherograms with signal intensities in RFU. The blue lines represent 50RFU that is the lower threshold for STR-typing at our department. The arrow in C indicates the presence of a fluorochrome derived artifact. Arrow in D indicates the position of the artifact if not purified. Forensic Science International: Genetics Supplement Series 2011 3, e347-e348DOI: (10.1016/j.fsigss.2011.09.036) Copyright © 2011 Elsevier Ireland Ltd Terms and Conditions

Fig. 2 The average signal intensities across all loci (RFU) pre- and post-PCR purification, ±std. dev. of non-purified (n=1) diluted amplificates electrophoresed with injection times of 10, 20 and 30s compared to purified (n=4) amplificates electrophoresed with 10s injection time. Forensic Science International: Genetics Supplement Series 2011 3, e347-e348DOI: (10.1016/j.fsigss.2011.09.036) Copyright © 2011 Elsevier Ireland Ltd Terms and Conditions