Structure of the MCS in backbone vectors for the BiFC plasmids.

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Run restriction digestion: TA's will take the pictures for you.

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Structure of the MCS in backbone vectors for the BiFC plasmids. Structure of the MCS in backbone vectors for the BiFC plasmids. (A) MCS for the generation of N-terminal fusions of the fluorophore to the genes of interest. The fluorophore part is cloned in the BspEI and NheI sites on the left. Genes of interest are cloned using the AvrII-XmaI-AscI-MluI cloning site on the right, separating them from the fluorophore part by the indicated linker sequence. (B) MCS for the generation of C-terminal fusions of the fluorophore to the genes of interest. The fluorophore part is cloned in the NheI and MluI sites on the right. Genes of interest are cloned using the BspEI-AvrII-XmaI-AscI cloning site on the left, separating them from the fluorophore part by the indicated linker sequence. (C) Schematic representation of all possible BiFC fusion orientations with either the N-terminal (NV) or C-terminal (CV) part of yEmVenus. Ana Subotić et al. G3 2017;7:3509-3520 ©2017 by Genetics Society of America