Cultured Human Dermal Fibroblasts do Produce Cortisol Andrzej Slominski, Blazej Zbytek, Andrzej Szczesniewski, Jacobo Wortsman  Journal of Investigative Dermatology  Volume 126, Issue 5, Pages 1177-1178 (May 2006) DOI: 10.1038/sj.jid.5700204 Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Effect of progesterone on cortisol synthesis. Fibroblasts were cultured in 24-well plates seeded at density of 105 cells/well. Differences between control and experimental treatments: *P<0.05. Journal of Investigative Dermatology 2006 126, 1177-1178DOI: (10.1038/sj.jid.5700204) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Liquid chromatography mass spectrometry (LC/MS2) identifies cortisol in cultures of fibroblasts. Fibroblasts were cultured in Petri dishes seeded at density of 2 × 106 cells/dish. (a, c) Cortisol standard and (b, d) extracts from conditioned media. Journal of Investigative Dermatology 2006 126, 1177-1178DOI: (10.1038/sj.jid.5700204) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions