Inositol Lipids: To PHix or Not to PHix? Robin Irvine  Current Biology  Volume 14, Issue 8, Pages R308-R310 (April 2004) DOI: 10.1016/j.cub.2004.03.051

Figure 1 Real-time visualisation of PtdIns(4,5)P2 hydrolysis. RBL cells have been transfected with the GFP-tagged PH domain of PI-PLC δ1, which specifically binds to the headgroup of PtdIns(4,5)P2, and thus it predominantly lights up the plasma membrane (left, 0sec). By 60 seconds after platelet-activating factor has been applied to the cells, most of the GFP-tagged protein has fallen off into the cytoplasm (right), indicating that PtdIns(4,5)P2 hydrolysis has taken place (see the text for a more detailed interpretation). Reproduced from Figure 3 in [2]. Current Biology 2004 14, R308-R310DOI: (10.1016/j.cub.2004.03.051)

Figure 2 Real-time visualisation of PtdIns(3,4,5)P3 production. Adipocytes (3T3 L1 cells) have been transfected with the GFP-tagged PH domain of ARNO, which specifically binds the headgroup of PtdIns(3,4,5)P3. In unstimulated cells this protein is mostly in the cytosol (control, left), but 60 seconds after insulin is applied it translocates mostly to the plasma membrane (right), indicating that synthesis of PtdIns(3,4,5)P3 has taken place. Reproduced from Figure 2 in [3]. Current Biology 2004 14, R308-R310DOI: (10.1016/j.cub.2004.03.051)