Melissa A. Mazur, Craig C. Davis, Paul Szabolcs, MD

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Presentation transcript:

Ex Vivo Expansion and Th1/Tc1 Maturation of Umbilical Cord Blood T Cells by CD3/CD28 Costimulation Melissa A. Mazur, Craig C. Davis, Paul Szabolcs, MD Biology of Blood and Marrow Transplantation Volume 14, Issue 10, Pages 1190-1196 (October 2008) DOI: 10.1016/j.bbmt.2008.07.016 Copyright © 2008 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 1 Phenotypic evolution, proliferation and apoptosis in the expanded T cell progeny. A 4-color FACS dotplot profile of viable T lymphocytes contrasting the starting day 0 and post-expansion day 14 progeny. Surface detection of indicated antibodies is presented except for Ki-67 and activated caspase-3, which were detected following permeabilization and intracellular staining. The relative size of the indicated T cell subsets in a quadrant is expressed as the percentage of total viable T cells. Representative experiment, n = 10. Biology of Blood and Marrow Transplantation 2008 14, 1190-1196DOI: (10.1016/j.bbmt.2008.07.016) Copyright © 2008 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 2 Th1/Tc1 cytokine secretion profile and Granzyme A expression in the expanded T cell progeny. A 4-color FACS dotplot profile of viable T lymphocytes contrasting the starting day 0 and postexpansion day 14 progeny. Surface detection of indicated antibodies is presented except for IL-2, TNFa, and Granzyme B, which were detected following permeabilization and intracellular staining. The relative size of the indicated T cell subsets in a quadrant is expressed as the percentage of total viable T cells. Representative experiment, n =10. Biology of Blood and Marrow Transplantation 2008 14, 1190-1196DOI: (10.1016/j.bbmt.2008.07.016) Copyright © 2008 American Society for Blood and Marrow Transplantation Terms and Conditions