Blood manufacturing methods affect red blood cell product characteristics and immunomodulatory activity by Ruqayyah J. Almizraq, Philip J. Norris, Heather.

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Presentation transcript:

Blood manufacturing methods affect red blood cell product characteristics and immunomodulatory activity by Ruqayyah J. Almizraq, Philip J. Norris, Heather Inglis, Somaang Menocha, Mathijs R. Wirtz, Nicole Juffermans, Suchitra Pandey, Philip C. Spinella, Jason P. Acker, and Jennifer A. Muszynski BloodAdv Volume 2(18):2296-2306 September 25, 2018 © 2018 by The American Society of Hematology

Ruqayyah J. Almizraq et al. Blood Adv 2018;2:2296-2306 © 2018 by The American Society of Hematology

Residual WBC and platelet counts in RCC produced by different manufacturing methods as measured on day 5 of storage. Residual WBC and platelet counts in RCC produced by different manufacturing methods as measured on day 5 of storage. Data reported for (A) residual white blood cells and (B) residual platelets as scatter dot plots with mean and standard deviation.. Ruqayyah J. Almizraq et al. Blood Adv 2018;2:2296-2306 © 2018 by The American Society of Hematology

Hemolysis and supernatant K+ of differently manufactured RCC products. Hemolysis and supernatant K+of differently manufactured RCC products. Dot plots display (A) percent hemolysis and (B) level of supernatant K+ on day 5 (fresh/white) and day 42 (expired/shaded) of stored and differently manufactured RCC products. Data reported as scatter dot plots with mean and standard deviation. *Significant results (P < .05) in comparison with day 5 values. δSignificant difference (P < .05) compared with the noted blood manufacturing methods. Ruqayyah J. Almizraq et al. Blood Adv 2018;2:2296-2306 © 2018 by The American Society of Hematology

Concentration of EV and subpopulation in RCC products stored for up to 42 days analyzed by the TRPS and flow cytometry systems. Concentration of EV and subpopulation in RCC products stored for up to 42 days analyzed by the TRPS and flow cytometry systems. (A) EV < 200 nm, (B) EV ≥ 200 nm, (C) total EV, (D) RBC-EV, (E) platelet-EV, and (F) WBC-EV. Data are reported as mean ± standard deviation. *Significant results (P < .05) in comparison with day 5 values. δSignificant difference (P < .05) compared with the noted blood manufacturing methods (n = 8 per blood manufacturing method). Ruqayyah J. Almizraq et al. Blood Adv 2018;2:2296-2306 © 2018 by The American Society of Hematology

Monocyte LPS-induced cytokine production. Monocyte LPS-induced cytokine production. (A) TNF-α, (B) IL-10, (C) IL-1β, and (D) IL-8 following exposure of RCC supernatant from different RCC manufacturing methods at fresh (day 5/white) and at expiration (day 42/shaded). Significant level in comparison with control (*P < .05; **P < .01; ***P < .001). Ruqayyah J. Almizraq et al. Blood Adv 2018;2:2296-2306 © 2018 by The American Society of Hematology

Monocyte IL-8 production in the absence of LPS stimulation and following exposure to RCC supernatant from different RCC manufacturing methods at fresh (day 5/white) and at expiration (day 42/shaded). *Significant level in comparison with control (***P < .001; ****P < .0001). Monocyte IL-8 production in the absence of LPS stimulation and following exposure to RCC supernatant from different RCC manufacturing methods at fresh (day 5/white) and at expiration (day 42/shaded). *Significant level in comparison with control (***P < .001; ****P < .0001). Ruqayyah J. Almizraq et al. Blood Adv 2018;2:2296-2306 © 2018 by The American Society of Hematology