(A) N. fowleri, from a patient with PAM, growing in axenic medium in tissue culture flasks. (A) N. fowleri, from a patient with PAM, growing in axenic.

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(A) N. fowleri, from a patient with PAM, growing in axenic medium in tissue culture flasks. (A) N. fowleri, from a patient with PAM, growing in axenic medium in tissue culture flasks. Amebas contain numerous fluid-filled vacuoles and appear larger than their counterparts growing on bacteria. The cultures were photographed in situ on an inverted microscope. Magnification, ×960; original magnification, ×1,000. (Micrograph copyright T. H. Dunnebacke.) (B) N. fowleri amebas in a wet-mount microscope slide. The prominent clear, ectoplasmic pseudopod is seen (arrow). Adjacent particles are bacteria. Magnification, ×960; original magnification, ×1,000. (C) Trophic A. castellanii ameba from culture. Amebas show the characteristic projecting pseudopods (acanthopodia) over the surface. Magnification, ×960; original magnification, ×1,000. (D) Interference-contrast image of cysts of Acanthamoeba sp. isolated from a human brain biopsy specimen. The cysts are on the surface of an agar plate. Magnification, ×1440; original magnification, ×1,500. (Micrographs B to D copyright G. S. Visvesvara.)‏ Frederick L. Schuster Clin. Microbiol. Rev. 2002; doi:10.1128/CMR.15.3.342-354.2002