A. Carannante, G. Prignano, M. Cusini, A. Matteelli, I. Dal Conte, V

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Cefixime and ceftriaxone susceptibility of Neisseria gonorrhoeae in Italy from 2006 to 2010 A. Carannante, G. Prignano, M. Cusini, A. Matteelli, I. Dal Conte, V. Ghisetti, A. D'Antuono, F. Cavrini, R. Antonetti, P. Stefanelli Clinical Microbiology and Infection Volume 18, Issue 6, Pages 558-564 (June 2012) DOI: 10.1111/j.1469-0691.2011.03619.x Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Distribution of cefixime MICs among Italian gonococci from 2006 to 2010, (a) and year by year (b). The in vitro EUCAST and CLSI breakpoints for resistance are indicated as dashed lines. Clinical Microbiology and Infection 2012 18, 558-564DOI: (10.1111/j.1469-0691.2011.03619.x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Homology tree obtained with por alleles among strains with MIC ≥0.125 mg/L associated with the 110 tbpB allele identifying the ST1407 by Neisseria gonorrhoeae multi-antigen sequence typing. Clinical Microbiology and Infection 2012 18, 558-564DOI: (10.1111/j.1469-0691.2011.03619.x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 3 Dendrogram of similarity obtained with Dice coefficients and arithmetic averages (UPGMA) clustering method using BIONUMERICS software (version 3.5) showing the relatedness of pulsed field gel electrophoresis patterns for representative Neisseria gonorrhoeae strains fully susceptible or resistant to cefixime. The profiles are classified into two major clusters (A and B). Isolates that produced patterns that were <85% similar (see scale in the upper left corner) were considered different. The isolate numbers, MICs and sequence types are indicated and referred to in Table 2. Clinical Microbiology and Infection 2012 18, 558-564DOI: (10.1111/j.1469-0691.2011.03619.x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions