Dr Brian J. Harvey, Maria Higgins Kidney International

Slides:

Advertisements

Similar presentations

Volume 63, Issue 4, Pages (April 2003)

Advertisements

Hepatocyte growth factor in renal failure: Promise and reality

Chronic exposure of human mesangial cells to high glucose environments activates the p38 MAPK pathway William A. Wilmer, Cynthia L. Dixon, Courtney Hebert

Effect of arginine vasopressin and ANP on intracellular pH and cytosolic free [Ca2+] regulation in MDCK cells Maria Oliveira-Souza, Margarida Mello-Aires,

The Role of Bile Acids in Gallstone-Induced Pancreatitis

Neal X. Chen, Kalisha D. O'Neill, Toshimitsu Niwa, Sharon M. Moe

Early aldosterone up-regulated genes: New pathways for renal disease?

Volume 53, Issue 5, Pages (May 1998)

Volume 59, Issue 1, Pages (January 2001)

Volume 79, Issue 9, Pages (May 2011)

Volume 86, Issue 1, Pages (July 2014)

IGF-1 vs insulin: Respective roles in modulating sodium transport via the PI-3 kinase/Sgk1 pathway in a cortical collecting duct cell line E. Gonzalez-Rodriguez,

Volume 75, Issue 1, Pages (January 2009)

Making sense of the sensor: Mysteries of the macula densa

Volume 67, Issue 6, Pages (June 2005)

Kinase activation and smooth muscle contraction in the presence and absence of calcium Glenn Whitney, MD, Doug Throckmorton, MD, Carlos Isales, MD, Yo.

Paolo Ferrari, M.D., Zygmunt Krozowski Kidney International

A trail of research on potassium

Volume 61, Issue 3, Pages (March 2002)

Renal and extrarenal regulation of potassium

ANO1: an additional key player in cyst growth

Volume 59, Issue 1, Pages (January 2001)

Volume 65, Issue 6, Pages (June 2004)

Volume 71, Issue 5, Pages (March 2007)

Kamel S. Kamel, Martin Schreiber, Mitchell L. Halperin

Volume 57, Issue 6, Pages (June 2000)

Volume 56, Issue 4, Pages (October 1999)

Steroid hormones: Interactions with membrane-bound receptors

Let's look at cysts from both sides now

Volume 6, Issue 8, Pages (August 1996)

Alison M.J. Buchan, Paul E. Squires, Mark Ring, R.Mark Meloche

Chronic exposure of human mesangial cells to high glucose environments activates the p38 MAPK pathway William A. Wilmer, Cynthia L. Dixon, Courtney Hebert

Volume 68, Issue 3, Pages (September 2005)

Volume 67, Issue 4, Pages (April 2005)

Estradiol suppresses type I collagen synthesis in mesangial cells via activation of activator protein-1 Sharon Silbiger, Jun Lei, Joel Neugarten Kidney.

Volume 76, Issue 8, Pages (October 2009)

Karl Kunzelmann, Rainer Schreiber, Anissa Boucherot

Volume 83, Issue 4, Pages (April 2013)

Disorders of the epithelial sodium channel: Insights into the regulation of extracellular volume and blood pressure Principal discussant: John B. Stokes

Volume 68, Issue 5, Pages (November 2005)

Heme oxygenase: protective enzyme or portal hypertensive molecule?

Signaling path of the action of AVP on distal K+ secretion

Volume 62, Issue 2, Pages (August 2002)

Volume 79, Issue 4, Pages (February 2011)

Calcium sensing in podocytes

Volume 84, Issue 2, Pages (August 2013)

Randall R. Sakai, Ph. D. , Bruce S. Mcewen, Steve J

Volume 63, Issue 4, Pages (April 2003)

Edward J. Weinman, Deborah Steplock, Shirish Shenolikar

Volume 57, Issue 1, Pages (January 2000)

EMT and proteinuria as progression factors

Ho Jae Han, Soo Hyun Park, Hyun Ju Koh, Mary Taub Kidney International

Volume 60, Issue 2, Pages (August 2001)

Volume 61, Issue 6, Pages (June 2002)

A possible mechanism of renal cell death after ischemia/reperfusion

Hydrogen peroxide activates ion currents in rat mesangial cells

G proteins and hypertension: An alternative candidate gene approach

Volume 67, Issue 1, Pages (January 2005)

A new regulator of the vacuolar H+-ATPase in the kidney

Volume 73, Issue 8, Pages (April 2008)

Miroslaw Smogorzewski, Varuzhan Galfayan, Shaul G. Massry

Volume 71, Issue 9, Pages (May 2007)

Volume 62, Issue 4, Pages (October 2002)

Volume 57, Issue 4, Pages (April 2000)

Aldosterone and potassium secretion by the cortical collecting duct

T cells and T-cell receptors in acute renal failure

Novel aspects of complement in kidney injury

Volume 55, Issue 1, Pages (January 1999)

Marie Leclerc, Michèle G. Brunette, Denis Couchourel

Volume 55, Issue 1, Pages (January 1999)

Presentation transcript:

Nongenomic effects of aldosterone on Ca2+ in M-1 cortical collecting duct cells Dr Brian J. Harvey, Maria Higgins Kidney International Volume 57, Issue 4, Pages 1395-1403 (April 2000) DOI: 10.1046/j.1523-1755.2000.00981.x Copyright © 2000 International Society of Nephrology Terms and Conditions

Figure 1 Representative Fura-2 spectrofluorescence recording of the increase in intracellular calcium stimulated by 1 nmol/L aldosterone (Aldo) in confluent M-1 cell monolayers (•). This increase was completely inhibited by pretreatment (15 min) with the protein kinase C inhibitor chelerythrine chloride (▪). Kidney International 2000 57, 1395-1403DOI: (10.1046/j.1523-1755.2000.00981.x) Copyright © 2000 International Society of Nephrology Terms and Conditions

Figure 2 Effect of aldosterone (1 nmol/L) on Ca2+ in M-1 cells in the presence of inhibitors of the genomic pathway, actinomycin D and spironolactone, and the protein kinase C (PKC) inhibitor chelerythrine chloride. Note that the PKC activator, phorbol myristyl acetate (PMA), produced a similar Ca2+ response to aldosterone. Kidney International 2000 57, 1395-1403DOI: (10.1046/j.1523-1755.2000.00981.x) Copyright © 2000 International Society of Nephrology Terms and Conditions

Figure 3 Steroid hormone effects on intracellular calcium in confluent M-1 cells. Aldosterone (1 nmol/L) induced a rapid increase in [Ca2+]i that was significantly different from that produced by dexamethasone (10 μmol/L), 17β;-estradiol (10 nmol/L), or progesterone (10 nmol/L). Hydrocortisone failed to increase [Ca2+]i significantly even at a concentration of 10 μmol/L. Kidney International 2000 57, 1395-1403DOI: (10.1046/j.1523-1755.2000.00981.x) Copyright © 2000 International Society of Nephrology Terms and Conditions

Figure 4 Cortical collecting duct (CCD) cell model of signals involved in the nongenomic pathway for steroid hormone action. Aldosterone binds to cytosolic or membrane-associated elements that may include PKC or a spironolactone-insensitive “receptor” to induce a nongenomic rise in intracellular calcium via a PKC-sensitive luminal entry of calcium and calcium release from intracellular stores. The calcium-kinase complex activates basolateral Na/H exchange, and the resultant intracellular alkalinization activates basolateral K+ channels. The increased K+ conductance primes the cell for the later genomic up-regulation of sodium absorption. Kidney International 2000 57, 1395-1403DOI: (10.1046/j.1523-1755.2000.00981.x) Copyright © 2000 International Society of Nephrology Terms and Conditions