DIA1(R1204X) induces elongated and thick microvilli in HeLa cells

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DIA1(R1204X) induces elongated and thick microvilli in HeLa cells DIA1(R1204X) induces elongated and thick microvilli in HeLa cells Various GFP‐tagged DIA1 plasmids were transfected into HeLa cells and fixed 24 h after transfection. Fixed cells were stained with Alexa568‐conjugated phalloidin and DAPI (blue) (A, B: representative of five experiments). Stress fiber formation was observed with a confocal laser microscope. Note the induction of stress fibers by GFP‐DIA1(M1190D) (asterisks). Arrow and arrowheads indicate a plasma membrane (PM) localization of GFP‐DIA1(M1190D) and a dot‐like localization of GFP‐DIA1(R1204X) adjacent to the PM, respectively. Inset in the top panel of DIA1(R1204X) shows the magnified image of the region indicated by the rectangle. Scale bars: 10 μm.Microvilli with or without GFP‐DIA1(R1204X) expression were observed under a confocal laser microscope. Note elongated and thick microvilli (arrowheads) in GFP‐DIA1(R1204X)‐transfected cells, but not in nontransfected cells (circles). 3D movies are available in Movie EV1 (R1204X), Movie EV2 (WT), and Movie EV3 (ttaa). Scale bar: 10 μm. Takehiko Ueyama et al. EMBO Mol Med. 2016;8:1310-1324 © as stated in the article, figure or figure legend