Biochemical characterization of motif VI mutants—R592A and H594A

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Biochemical characterization of motif VI mutants—R592A and H594A Biochemical characterization of motif VI mutants—R592A and H594A (A) Clustal W analysis of motif VI in SWI2/SNF2 proteins [41]. (B) Comparison of ATPase activity of R592A and H594A with wild-type ADAAD. (C) Comparison of the CD spectra of R592A with wild-type ADAAD in the absence of ligands. (D) Comparison of the CD spectra of R592A with wild-type ADAAD in the presence of ligands. (E) Comparison of kinetic data of H594A with the wild-type protein. (F) Comparison of the CD spectra of H594A with wild-type ADAAD in the absence of ligands. (G) Comparison of CD spectra of H594A with wild-type ADAAD in the presence of ligands. Ritu Bansal et al. Biosci. Rep. 2018;38:BSR20180568 ©2018 by Portland Press Ltd