The L2b real-time PCR targeting the pmpH gene of Chlamydia trachomatis used for the diagnosis of lymphogranuloma venereum is not specific to L2b strains

Slides:

Advertisements

Similar presentations

Models for the organisation of hospital infection control and prevention programmes B. Gordts Clinical Microbiology and Infection Volume 11, Pages

Advertisements

Laboratory diagnosis and biosafety issues of biological warfare agents

R. Dumke, H. von Baum, P.C. Lück, E. Jacobs

A. Bergman, D. Heimer, N. Kondori, H. Enroth

I.J. Schalk Clinical Microbiology and Infection

Approach to diagnosis of infective endocarditis

D. Khalil, M. Hultin, M.U. Rashid, B. Lund

Changes in the intestinal microbiota from adulthood through to old age

M. Cogliati, R. D'Amicis, A.M. Tortorano

Herpes zoster in non-hospitalized children

Risk factors for cryptococcal infection among patients with rheumatoid arthritis receiving different immunosuppressive medications T.-L. Liao, Y.-M.

Migrant health—a cause for concern?

Three-year retrospective analysis of the incidence of Toxoplasma gondii infection in pregnant women living in the Greater Romagna Area (northeastern Italy)

A.G. Koek, S.M. Bruisten, M. Dierdorp, A.P. van Dam, K. Templeton

C. Rodó, A. Suy, E. Sulleiro, A. Soriano-Arandes, A. Antón, I

A.K. Reddy, P. Garg, I. Kaur Clinical Microbiology and Infection

A comparison of three real-time PCR assays for the confirmation of Neisseria gonorrhoeae following detection of N. gonorrhoeae using Roche COBAS AMPLICOR

Development of a real-time PCR targeting the yidC gene for the detection of Mycoplasma hominis and comparison with quantitative culture C. Férandon,

A. F. Simonetti, C. Garcia-Vidal, D. Viasus, D. García-Somoza, J

Genital Chlamydia trachomatis infections

Clinical and epidemiological characterization of a lymphogranuloma venereum outbreak in Madrid, Spain: co-circulation of two variants M. Rodríguez-Domínguez,

Louis Pasteur, from crystals of life to vaccination

Chlamydia trachomatis infections in heterosexuals attending sexually transmitted disease clinics in Slovenia D. Kese, M. Maticic, M. Potocnik Clinical.

Dermatitis by Dermatophilus congolensis

C. Rodó, A. Suy, E. Sulleiro, A. Soriano-Arandes, A. Antón, I

Should standardized susceptibility testing for microbial biofilms be introduced in clinical practice? T. Coenye, D. Goeres, F. Van Bambeke, T. Bjarnsholt

Rapid detection of the recently emerged Bordetella pertussis strains with the ptxP3 pertussis toxin promoter allele by real-time PCR T. Kallonen, J.

H. Adibhesami, M. Douraghi, M. Rahbar, A. Abdollahi

Identification of genetic markers of resistance to echinocandins, azoles and 5- fluorocytosine in Candida glabrata by next-generation sequencing: a feasibility.

Vector control: a cornerstone in the malaria elimination campaign

Transmitted drug-resistance in human immunodeficiency virus-infected adult population in El Salvador, Central America Á. Holguín, G. Yebra, L. Martín,

A comparison of three real-time PCR assays for the confirmation of Neisseria gonorrhoeae following detection of N. gonorrhoeae using Roche COBAS AMPLICOR

Unexpectedly high prevalence of Treponema pallidum infection in the oral cavity of human immunodeficiency virus-infected patients with early syphilis.

F. Aujoulat, H. Marchandin, I. Zorgniotti, A. Masnou, E. Jumas-Bilak

Training for the infectious diseases speciality in Norway

Lymphogranuloma venereum variant L2b-specific polymerase chain reaction: insertion used to close an epidemiological gap S.P. Verweij, A. Catsburg, S.

Leucocyte esterase, glucose and C-reactive protein in the diagnosis of prosthetic joint infections: a prospective study E. De Vecchi, F. Villa, M. Bortolin,

Laboratory diagnosis and biosafety issues of biological warfare agents

Spread of a new Chlamydia trachomatis variant from men who have sex with men to the heterosexual population after replacement and recombination in ompA.

F. Magurano Clinical Microbiology and Infection

S. Imbert, J.-Y. Brossas, M. Palous, I. Joly, I. Meyer, A. Fekkar

New diagnostic tools in schistosomiasis

Metagenomics and probiotics

M. Aires-de-Sousa Clinical Microbiology and Infection

Previously unknown species of Aspergillus

Laboratory diagnosis of Clostridium difficile disease

Detection of Wolbachia genes in a patient with non-Hodgkin's lymphoma

Sexually acquired Zika virus: a systematic review

Systematic review of antibiotic consumption in acute care hospitals

Characterization of clinical strains of Aspergillus terreus complex: molecular identification and antifungal susceptibility to azoles and amphotericin.

Y. Huang, B. Yang, W. Li Clinical Microbiology and Infection

R. Slinger, I. Moldovan, N. Barrowman, F. Chan

Accurate hepatitis C virus genotyping and selection of optimal therapy: lessons from a St Petersburg strain infection E. Knops, E. Heger Clinical Microbiology.

Vaccinations against respiratory tract infections at Hajj

A.P. Underwood, J. Green Clinical Microbiology and Infection

Dynamics of hepatitis B virus quasispecies heterogeneity in association with nucleos(t)ide analogue treatment determined by MALDI-TOF MS M. Rybicka,

Increasing numbers of hospitalizations and deaths with mention of Clostridium difficile infection, north-eastern Italy, 2008–2013 U. Fedeli, E. Schievano,

Clinical Microbiology and Infection

I. Bitar, A. Piazza Clinical Microbiology and Infection

Survival of rhinoviruses on human fingers

The atypical pneumonias: clinical diagnosis and importance

Abstracts Clinical Microbiology and Infection

Virology: a scientific discipline facing new challenges

R. Khatib, G. Simeunovic, M. Sharma, M. G. Fakih, L. B. Johnson, L

J.L. Balcázar Clinical Microbiology and Infection

Three years experience of real-time PCR for the diagnosis of Q fever

Typing of Clostridium difficile

A. Vankeerberghen, S. Jonckheere, H. De Raeve, R. Caluwe, H

CMI readers' survey Clinical Microbiology and Infection

The future of diagnostic bacteriology

Presentation transcript:

The L2b real-time PCR targeting the pmpH gene of Chlamydia trachomatis used for the diagnosis of lymphogranuloma venereum is not specific to L2b strains A. Touati, O. Peuchant, N. Hénin, C. Bébéar, B. de Barbeyrac Clinical Microbiology and Infection Volume 22, Issue 6, Pages 574.e7-574.e9 (June 2016) DOI: 10.1016/j.cmi.2016.03.018 Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 1 Partial ompA gene sequences (position 400 to 500) of three L2-confirmed clinical specimens, Co5, TG93 and SL296, aligned against L2b/UCH-1 (AM884177) and L2/434/Bu (AM884176). Highlighted in grey is the A/G substitution at position 485 specific of L2b variant. Clinical Microbiology and Infection 2016 22, 574.e7-574.e9DOI: (10.1016/j.cmi.2016.03.018) Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 2 Partial pmpH gene sequence of the L2-positive clinical specimen TG93 aligned against L2b/142189 (EF612788.1), L2b/190437 (EF534758), L2/434/Bu (NC_010287.1, L1/440 (AY184167), L3/404 (AY184169), H (AY184163) and G (AY184162). Highlighted in grey are the nine nucleotides specific of L2b strains, but also found in the L2-positive clinical specimen. Clinical Microbiology and Infection 2016 22, 574.e7-574.e9DOI: (10.1016/j.cmi.2016.03.018) Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions